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Title: Suppressor tRNAs at the interface of genetic code expansion and medicine
Suppressor transfer RNAs (sup-tRNAs) are receiving renewed attention for their promising therapeutic properties in treating genetic diseases caused by nonsense mutations. Traditionally, sup-tRNAs have been created by replacing the anticodon sequence of native tRNAs with a suppressor sequence. However, due to their complex interactome, considering other structural and functional tRNA features for design and engineering can yield more effective sup-tRNA therapies. For over 2 decades, the field of genetic code expansion (GCE) has created a wealth of knowledge, resources, and tools to engineer sup-tRNAs. In this Mini Review, we aim to shed light on how existing knowledge and strategies to develop sup-tRNAs for GCE can be adopted to accelerate the discovery of efficient and specific sup-tRNAs for medical treatment options. We highlight methods and milestones and discuss how these approaches may enlighten the research and development of tRNA medicines.  more » « less
Award ID(s):
2304710
PAR ID:
10516945
Author(s) / Creator(s):
; ;
Publisher / Repository:
Frontiers
Date Published:
Journal Name:
Frontiers in Genetics
Volume:
15
ISSN:
1664-8021
Format(s):
Medium: X
Sponsoring Org:
National Science Foundation
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