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1. Peak trabecular bone microstructure predicts rate of estrogen-deficiency-induced bone loss in rats

   https://doi.org/10.1016/j.bone.2021.115862  

   Li, Yihan; Tseng, Wei-Ju; de Bakker, Chantal M.J.; Zhao, Hongbo; Chung, Rebecca; Liu, X. Sherry (April 2021, Bone)

   null (Ed.)
2. Patient-Derived Breast Cancer Bone Metastasis In Vitro Model Using Bone-Mimetic Nanoclay Scaffolds

   https://doi.org/10.1155/2023/5753666  

   Jasuja, Haneesh; Solaymani Mohammadi, Farid; Kim, Jiha; Gaba, Anu; Katti, Dinesh R.; Katti, Kalpana S. (March 2023, Journal of Tissue Engineering and Regenerative Medicine)

   Stabler, Cherie L. (Ed.)

   The unavailability of reliable models for studying breast cancer bone metastasis is the major challenge associated with poor prognosis in advanced-stage breast cancer patients. Breast cancer cells tend to preferentially disseminate to bone and colonize within the remodeling bone to cause bone metastasis. To improve the outcome of patients with breast cancer bone metastasis, we have previously developed a 3D in vitro breast cancer bone metastasis model using human mesenchymal stem cells (hMSCs) and primary breast cancer cell lines (MCF-7 and MDAMB231), recapitulating late-stage of breast cancer metastasis to bone. In the present study, we have tested our model using hMSCs and patient-derived breast cancer cell lines (NT013 and NT023) exhibiting different characteristics. We investigated the effect of breast cancer metastasis on bone growth using this 3D in vitro model and compared our results with previous studies. The results showed that NT013 and NT023 cells exhibiting hormone-positive and triple-negative characteristics underwent mesenchymal to epithelial transition (MET) and formed tumors in the presence of bone microenvironment, in line with our previous results with MCF-7 and MDAMB231 cell lines. In addition, the results showed upregulation of Wnt-related genes in hMSCs, cultured in the presence of excessive ET-1 cytokine released by NT013 cells, while downregulation of Wnt-related genes in the presence of excessive DKK-1, released by NT023 cells, leading to stimulation and abrogation of the osteogenic pathway, respectively, ultimately mimicking different types of bone lesions in breast cancer patients. 

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3. Bone toughness changes during skeletal growth in brittle osteogenesis imperfecta bone. How and why?

   Docaj, A.; Jeong, M.S.; Zimmermann, E.A.; Ritchie, R.O.; Carriero, A. (January 2021, IntOI 2020+1: Virtual International OI Meeting Early-Stage Investigator Symposium)
4. Piezoelectric Signals in Vascularized Bone Regeneration

   https://doi.org/10.3390/biom11111731  

   D’Alessandro, Delfo; Ricci, Claudio; Milazzo, Mario; Strangis, Giovanna; Forli, Francesca; Buda, Gabriele; Petrini, Mario; Berrettini, Stefano; Uddin, Mohammed Jasim; Danti, Serena; et al (November 2021, Biomolecules)

   The demand for bone substitutes is increasing in Western countries. Bone graft substitutes aim to provide reconstructive surgeons with off-the-shelf alternatives to the natural bone taken from humans or animal species. Under the tissue engineering paradigm, biomaterial scaffolds can be designed by incorporating bone stem cells to decrease the disadvantages of traditional tissue grafts. However, the effective clinical application of tissue-engineered bone is limited by insufficient neovascularization. As bone is a highly vascularized tissue, new strategies to promote both osteogenesis and vasculogenesis within the scaffolds need to be considered for a successful regeneration. It has been demonstrated that bone and blood vases are piezoelectric, namely, electric signals are locally produced upon mechanical stimulation of these tissues. The specific effects of electric charge generation on different cells are not fully understood, but a substantial amount of evidence has suggested their functional and physiological roles. This review summarizes the special contribution of piezoelectricity as a stimulatory signal for bone and vascular tissue regeneration, including osteogenesis, angiogenesis, vascular repair, and tissue engineering, by considering different stem cell sources entailed with osteogenic and angiogenic potential, aimed at collecting the key findings that may enable the development of successful vascularized bone replacements useful in orthopedic and otologic surgery. 

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5. Increased deformations are dispensable for encapsulated cell mechanoresponse in engineered bone analogs mimicking aging bone marrow

   https://doi.org/10.1016/j.mbm.2024.100097  

   Regner, Alexander M; DeLeon, Maximilien; Gibbons, Kalin D; Howard, Sean; Nesbitt, Derek Q; Darghiasi, Seyedeh F; Zavala, Anamaria G; Lujan, Trevor J; Fitzpatrick, Clare K; Farach-Carson, Mary C; et al (March 2025, Mechanobiology in Medicine)