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Title: Hydrophobic Clusters Regulate Surface Hydration Dynamics of Bacillus subtilis Lipase A
The surface hydration diffusivity of Bacillus subtilis Lipase A (BSLA) has been characterized by low-field Overhauser dynamic nuclear polarization (ODNP) relaxometry using a series of spin-labeled constructs. Sites for spin-label incorporation were previously designed via an atomistic computational approach that screened for surface exposure, reflective of the surface hydration comparable to other proteins studied by this method, as well as minimal impact on protein function, dynamics, and structure of BSLA by excluding any surface site that participated in greater than 30% occupancy of a hydrogen bonding network within BSLA. Experimental ODNP relaxometry coupling factor results verify the overall surface hydration behavior for these BSLA spin-labeled sites similar to other globular proteins. Here, by plotting the ODNP parameters of relative diffusive water versus the relative bound water, we introduce an effective "phase-space" analysis, which provides a facile visual comparison of the ODNP parameters of various biomolecular systems studied to date. We find notable differences when comparing BSLA to other systems, as well as when comparing different clusters on the surface of BSLA. Specifically, we find a grouping of sites that correspond to the spin-label surface location within the two main hydrophobic core clusters of the branched aliphatic amino acids isoleucine, leucine, and valine cores observed in the BSLA crystal structure. The results imply that hydrophobic clustering may dictate local surface hydration properties, perhaps through modulation of protein conformations and samplings of the unfolded states, providing insights into how the dynamics of the hydration shell is coupled to protein motion and fluctuations.  more » « less
Award ID(s):
2003366
PAR ID:
10521515
Author(s) / Creator(s):
; ;
Corporate Creator(s):
Editor(s):
na
Publisher / Repository:
Journal of Physical Chemistry B
Date Published:
Journal Name:
The Journal of Physical Chemistry B
Edition / Version:
1
Volume:
128
Issue:
16
ISSN:
1520-6106
Page Range / eLocation ID:
3919 to 3928
Subject(s) / Keyword(s):
cluster chemistry hydration hydrophobicity peptides and proteins EPR spin-labeling
Format(s):
Medium: X Size: 4MB Other: pdf
Size(s):
4MB
Sponsoring Org:
National Science Foundation
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