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1. Sequential anaerobic and aerobic bioaugmentation for commingled groundwater contamination of trichloroethene and 1,4-dioxane

   https://doi.org/10.1016/j.scitotenv.2021.145118  

   Li, Fei; Deng, Daiyong; Zeng, Lingke; Abrams, Stewart; Li, Mengyan (June 2021, Science of The Total Environment)

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2. Distinct Catalytic Behaviors between Two 1,4-Dioxane-Degrading Monooxygenases: Kinetics, Inhibition, and Substrate Range

   https://doi.org/10.1021/acs.est.9b05671  

   Li, Fei; Deng, Daiyong; Li, Mengyan (December 2019, Environmental Science & Technology)
3. Identification of the phylotypes involved in cis-dichloroethene and 1,4-dioxane biodegradation in soil microcosms.

   https://doi.org/10.1016/j.scitotenv.2021.148690  

   Dang, Hongyu and (November 2021, Science of the total environment)

   Co-contamination with chlorinated compounds and 1,4-dioxane has been reported at many sites. Recently, there has been an increased interest in bioremediation because of the potential to degrade multiple contaminants concurrently. Towards improving bioremediation efficacy, the current study examined laboratory microcosms (inoculated separately with two soils) to determine the phylotypes and functional genes associated with the biodegradation of two common co-contaminants (cis-dichloroethene [cDCE] and 1,4-dioxane). The impact of amending microcosms with lactate on cDCE and 1,4-dioxane biodegradation was also investigated. The presence of either lactate or cDCE did not impact 1,4-dioxane biodegradation one of the two soils. Lactate appeared to improve the initiation of the biological removal of cDCE in microcosms inoculated with either soil. Stable isotope probing (SIP) was then used to determine which phylotypes were actively involved in carbon uptake from cDCE and 1,4-dioxane in both soil communities. The most enriched phylotypes for 13C assimilation from 1,4-dioxane included Rhodopseudomonas and Rhodanobacter. Propane monooxygenase was predicted (by PICRUSt2) to be dominant in the 1,4-dioxane amended microbial communities and propane monooxygenase gene abundance values correlated with other enriched (but less abundant) phylotypes for 13C-1,4-dioxane assimilation. The dominant enriched phylotypes for 13C assimilation from cDCE included Bacteriovorax, Pseudomonas and Sphingomonas. In the cDCE amended soil microcosms, PICRUSt2 predicted the presence of DNA encoding glutathione S-transferase (a known cDCE upregulated enzyme). Overall, the work demonstrated concurrent removal of cDCE and 1,4-dioxane by indigenous soil microbial communities and the enhancement of cDCE removal by lactate. The data generated on the phylotypes responsible for carbon uptake (as determined by SIP) could be incorporated into diagnostic molecular methods for site characterization. The results suggest concurrent biodegradation of cDCE and 1,4-dioxane should be considered for chlorinated solvent site remediation. 

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4. Emerging investigator series: environment-specific auxiliary substrates tailored for effective cometabolic bioremediation of 1,4-dioxane

   https://doi.org/10.1039/D2EW00524G  

   Deng, Daiyong; Pham, Dung Ngoc; Li, Mengyan (January 2022, Environmental Science: Water Research & Technology)

   Cometabolic bioremediation is trending for the treatment of 1,4-dioxane (dioxane) and other emerging contaminants to meet stringent regulatory goals ( e.g. , <10 μg L −1 ) since biodegradation activities can be fueled by the supplementation of auxiliary substrates. In this study, we compared and investigated the effectiveness of two types of common auxiliary substrates, short-chain alkane gases ( e.g. , propane and butane) and primary alcohols ( e.g. , 1-propanol, 1-butanol, and ethanol), for dioxane removal in diverse environmental matrices with Azoarcus sp. DD4 as the inoculum. Physicochemical characterization at the pure culture level revealed that propane and 1-propanol are advantageous for stimulating cell growth and dioxane biodegradation by DD4. Parallel microcosm assays were conducted to assess the compatibility of DD4 bioaugmentation in diverse microbiomes recovered from five different environmental samples, including shallow and deep aquifer groundwater, contaminated river sediment, and municipal activated sludge. Propane was effective in sustaining efficient dioxane removal and the dominance of DD4 across all environmental matrices. Notably, amendment with 1-propanol promoted superior dioxane degradation in the deep aquifer groundwater, in which low pre-treatment biomass and post-treatment diversity were observed, suggesting its potential for intrinsic field applications. The combination of microbial community analysis and differential ranking identified that Ochrobactrum and several other indigenous bacteria were boosted by the inoculation of DD4, implying their commensal or mutualistic relationship. Collectively, propane and 1-propanol can be effective auxiliary substrate alternatives tailored for in situ bioaugmentation and their effectiveness is affected by the density and structure of environmental microbiomes. 

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5. Low-temperature persulfate activation by powdered activated carbon for simultaneous destruction of perfluorinated carboxylic acids and 1,4-dioxane

   https://doi.org/10.1016/J.JHAZMAT.2022.129966  

   Manz, Katherine E.; Kulaots, Indrek; Greenley, Caroline A.; Landry, Patrick J.; Lakshmi, K.V.; Woodcock, Matthew J.; Hellerich, Lucas; Bryant, J. Daniel; Apfelbaum, Mike; Pennell, Kurt D. (January 2023, Journal of Hazardous Materials)