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A sensitive, noninvasive method to detect localized prostate cancer, particularly for early detection and repetitive study in patients undergoing active surveillance, remains an unmet need. Here, we propose a molecular photoacoustic (PA) imaging approach by targeting the prostate‐specific membrane antigen (PSMA), which is over‐expressed in the vast majority of prostate cancers. We performed spectroscopic PA imaging in an experimental model of prostate cancer, namely, in immunocompromised mice bearing PSMA+ (PC3 PIP) and PSMA− (PC3 flu) tumors through administration of the known PSMA‐targeted fluorescence agent, YC‐27. Differences in contrast between PSMA+ and isogenic control tumors were observed upon PA imaging, with PSMA+ tumors showing higher contrast in average of 66.07‐fold with 5 mice at the 24‐hour postinjection time points. These results were corroborated using standard near‐infrared fluorescence imaging with YC‐27, and the squared correlation between PA and fluorescence intensities was 0.89. Spectroscopic PA imaging is a new molecular imaging modality with sufficient sensitivity for targeting PSMAin vivo, demonstrating the potential applications for other saturable targets relevant to cancer and other disorders.
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A nitroreductase-sensitive near-IR fluorescent biosensor for detecting tumor hypoxia in vivo
The near-IR fluorescent biosensor is highly selective and sensitive in responding to the hypoxic microenvironment of tumors, demonstrating high efficiency in detecting tumor hypoxia and the capability of distinguishing tumors of different sizes.
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- PAR ID:
- 10552864
- Publisher / Repository:
- RSC
- Date Published:
- Journal Name:
- Sensors & Diagnostics
- Volume:
- 3
- Issue:
- 9
- ISSN:
- 2635-0998
- Page Range / eLocation ID:
- 1505 to 1512
- Format(s):
- Medium: X
- Sponsoring Org:
- National Science Foundation
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- Free Publicly Accessible Full Text
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Accepted Manuscript1.0
- Journal Article:
- https://doi.org/10.1039/D4SD00146J
