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Abstract The inverted repeat (IR) lacking clade (IRLC) is a monophyletic group within the Papilionoideae subfamily of Fabaceae where plastid genomes (plastomes) do not contain the large IR typical of land plants. Recently, an IRLC legume,Medicago minima, was found to have regrown a ~9 kb IR that contained a number of canonical IR genes, and closely relatedM. lupulinacontained an incomplete IR of ~425 bp. Complete plastomes were generated for seven additional species, putative members of theM. minimaclade. Polymerase chain reaction was employed to investigate the presence of the IR acrossM. minimaandM. lupulinaincluding individuals of nine and eight Eurasian and North African accessions and 15 and 14 Texas populations, respectively. While no sequence similar to the ~9 kb IR was detected among the seven newly sequenced plastomes, all Eurasian and North African accessions ofM. minimacontained the IR. Variation in IR extent was detected within and between the Texas populations. Expansions of 13 bp and 11 bp occurred at the boundaries of both IR/small single‐copy regions, and populations had one or the other expansion, but not both. Expansion of the IR was not detected in the accessions from Eurasia and North Africa suggesting recent mutations yielded at least two additional plastid haplotypes inM. minima.
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The complete mitochondrial genomes of two imperiled species endemic to the Southwestern United States: Peppered Chub ( Macrhybopsis tetranema ) and Gila Trout ( Oncorhynchus gilae )
Macrhybopsis tetranema and Oncorhynchus gilae are fish species endemic to the Southwestern United States. We present the complete mitochondrial genomes for these species. Each genome consisted of 13 protein-coding genes, two ribosomal (rRNA) genes, 22 transfer RNA (tRNA) genes, and the control region (D-loop). Mitogenome lengths were 16,916 base pairs (bp) for M. tetranema, and 16,976 bp for O. gilae. The GC content was 41% for M. tetranema and 46% for O. gilae. The relationships of M. tetranema and O. gilae were consistent with previous phylogenetic analyses.
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- Award ID(s):
- 2208923
- PAR ID:
- 10555938
- Publisher / Repository:
- MITOCHONDRIAL DNA PART B
- Date Published:
- Journal Name:
- Mitochondrial DNA Part B
- Volume:
- 8
- Issue:
- 8
- ISSN:
- 2380-2359
- Page Range / eLocation ID:
- 809 to 814
- Format(s):
- Medium: X
- Sponsoring Org:
- National Science Foundation
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- Free Publicly Accessible Full Text
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Accepted Manuscript1.0
- Journal Article:
- https://doi.org/10.1080/23802359.2023.2241658
