An official website of the United States government
Abstract A novel budding yeast species was isolated from a soil sample collected in the United States of America. Phylogenetic analyses of multiple loci and phylogenomic analyses conclusively placed the species within the genusPichia. Strain yHMH446 falls within a clade that includesPichia norvegensis,Pichia pseudocactophila,Candida inconspicua, andPichia cactophila. Whole genome sequence data were analyzed for the presence of genes known to be important for carbon and nitrogen metabolism, and the phenotypic data from the novel species were compared to allPichiaspecies with publicly available genomes. Across the genus, including the novel species candidate, we found that the inability to use many carbon and nitrogen sources correlated with the absence of metabolic genes. Based on these results,Pichia galeolatasp. nov. is proposed to accommodate yHMH446T(=NRRL Y‐64187 = CBS 16864). This study shows how integrated taxogenomic analysis can add mechanistic insight to species descriptions.
more »
« less
This content will become publicly available on November 27, 2025
Draft genome sequences for Neonectria magnoliae and Neonectria punicea, canker pathogens of Liriodendron tulipifera and Acer saccharum in West Virginia
ABSTRACT The fungal genusNeonectriacontains many phytopathogenic species currently impacting forests and fruit trees worldwide. Despite their importance, a majority ofNeonectriaspp. lack sufficient genomic resources to resolve suspected cryptic species. Here, we report draft genomes and assemblies forNeonectria magnoliaeNRRL 64651 andNeonectria puniceaNRRL 64653.
more »
« less
- Award ID(s):
- 2125066
- PAR ID:
- 10563523
- Editor(s):
- Hudson, André O
- Publisher / Repository:
- ASM Press
- Date Published:
- Journal Name:
- Microbiology Resource Announcements
- ISSN:
- 2576-098X
- Page Range / eLocation ID:
- e0104224
- Format(s):
- Medium: X
- Sponsoring Org:
- National Science Foundation
More Like this
-
-
Abstract AimWe studied the niche evolution and diversification modes in transisthmianAlpheusshrimps by examining the interplay between environmental niche divergence and conservatism in allopatric sister species. In a broader perspective, the current study analysed the evolution of climatic niche and the role of the environment in species diversification ofAlpheustransisthmian shrimp. LocationAtlantic and Eastern‐Pacific oceans. TaxonAlpheusshrimps (Caridea: Alpheidae). MethodsWe assembled georeferenced occurrences for 33 species ofAlpheus(with 24 sister species) from a time‐calibrated molecular phylogeny. We modelled their ecological niches and assessed niche overlap through pairwise comparisons. Additionally, we performed phylogenetic reconstructions of the ancestral environmental niche, for each niche axis. ResultsOur results demonstrate that thermal tolerances, food availability and hydrodynamic forces were relevant environmental axes in evolutionary processes in transisthmian species ofAlpheus. Among the 528 paired comparisons, we found that most niches were divergent, including in 12 clades formed by pairs of sister species (in only two of these clades were the niches fully equivalent). Phylogenetic reconstructions of ancestral niches showed an initial niche conservatism in all axes, with divergences intensifying in the last 12 million years. Main ConclusionsWe found evidence that confirms the relevance of the environmental changes that occurred in the West Atlantic and East Pacific for niche evolution in transisthmianAlpheusspecies, as well as for the emergence of some lineages. Our findings provide evidence for different modes ofAlpheusspecies speciation in a period consistent with the closure of the Isthmus of Panama.more » « less
-
PremiseThe genusAntennariahas a complex evolutionary history due to dioecism, excessive polyploidy, and the evolution of polyploid agamic complexes. We developed microsatellite markers fromA. corymbosato investigate genetic diversity and population genetic structure inAntennariaspecies. Methods and ResultsTwenty‐four novel microsatellite markers (16 nuclear and eight chloroplast) were developed fromA. corymbosausing an enriched genomic library. Ten polymorphic nuclear markers were used to characterize genetic variation in five populations ofA. corymbosa. One to four alleles were found per locus, and the expected heterozygosity and fixation index ranged from 0.00 to 0.675 and −0.033 to 0.610, respectively. We were also able to successfully amplify these markers in five additionalAntennariaspecies. ConclusionsThese markers are promising tools to study the population genetics of sexualAntennariaspecies and to investigate interspecific gene flow, clonal diversity, and parentage ofAntennariapolyploid agamic complexes.more » « less
-
PremiseTheSphagnum recurvumcomplex comprises a group of closely related peat mosses that are dominant components of many northern wetland ecosystems. Taxonomic hypotheses for the group range from interpreting the whole complex as one polymorphic species to distinguishing 6–10 species. The complex occurs throughout the Northern Hemisphere, and some of the putative species have intercontinental ranges. Our goals were to delimit the complex and assess its phylogenetic structure in relation to morphologically defined species and intercontinental geography. MethodsRADseq analyses were applied to a sample of 384 collections from Europe, North America, and Asia. The data were subjected to maximum likelihood phylogenetic analyses and analyses of genetic structure using the software STRUCTURE and multivariate ordination approaches. ResultsTheS. recurvumcomplex includesS. angustifolium,S. fallax,S. flexuosum,S. pacificum, andS. recurvumas clades with little evidence of admixture. We also resolved an unnamed clade that is referred to here asS. “pseudopacificum.” We confirm thatS. balticumandS. obtusumare nested within the complex. Species with bluntly acute to obtuse stem leaf apices are sister to those with acute to apiculate leaves. Most of the species exhibit some differentiation between intraspecific population systems disjunct on different continents. ConclusionsWe recognize seven species in the amendedS. recurvumcomplex, includingS. balticumandS. obtusum, in addition to the informal cladeS. “pseudopacificum.” Although we detected some geographically correlated phylogenetic structure within widespread morphospecies, our RADseq data support the interpretation that these species have intercontinental geographic ranges.more » « less
-
Abstract PremiseSphagnum magellanicum(Sphagnaceae, Bryophyta) has been considered to be a single semi‐cosmopolitan species, but recent molecular analyses have shown that it comprises a complex of at least seven reciprocally monophyletic groups, that are difficult or impossible to distinguish morphologically. MethodsNewly developed barcode markers and RADseq analyses were used to identify species among 808 samples from 119 sites. Molecular approaches were used to assess the geographic ranges of four North American species, the frequency at which they occur sympatrically, and ecological differentiation among them. Microhabitats were classified with regard to hydrology and shade. Hierarchical modelling of species communities was used to assess climate variation among the species. Climate niches were projected back to 22,000 years BP to assess the likelihood that the North American species had sympatric ranges during the late Pleistocene. ResultsThe species exhibited parallel morphological variation, making them extremely difficult to distinguish phenotypically. Two to three species frequently co‐occurred within peatlands. They had broadly overlapping microhabitat and climate niches. Barcode‐ versus RADseq‐based identifications were in conflict for 6% of the samples and always involvedS. diabolicumvs.S. magniae. ConclusionsThese species co‐occur within peatlands at scales that could permit interbreeding, yet they remain largely distinct genetically and phylogenetically. The four cryptic species exhibited distinct geographic and ecological patterns. Conflicting identifications from barcode vs. RADseq analyses forS. diabolicumversusS. magniaecould reflect incomplete speciation or hybridization. They comprise a valuable study system for additional work on climate adaptation.more » « less
