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Non-destructive measurements of internal morphological structures in plant materials such as seeds are of high interest in agricultural research. The estimation of pericarp thickness is important to understand the grain quality and storage stability of seeds and can play a crucial role in improving crop yield. In this study, we demonstrate the applicability of fiber-based Bessel beam Fourier domain (FD) optical coherence microscopy (OCM) with a nearly constant high lateral resolution maintained at over ~400 µm for direct non-invasive measurement of the pericarp thickness of two different sorghum genotypes. Whereas measurements based on axial profiles need additional knowledge of the pericarp refractive index, en-face views allow for direct distance measurements. We directly determine pericarp thickness from lateral sections with a 3 µm resolution by taking the width of the signal corresponding to the pericarp at the 1/e threshold. These measurements enable differentiation of the two genotypes with 100% accuracy. We find that trading image resolution for acquisition speed and view size reduces the classification accuracy. Average pericarp thicknesses of 74 µm (thick phenotype) and 43 µm (thin phenotype) are obtained from high-resolution lateral sections, and are in good agreement with previously reported measurements of the same genotypes. Extracting the morphological features of plant seeds using Bessel beam FD-OCM is expected to provide valuable information to the food processing industry and plant breeding programs.
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This content will become publicly available on March 3, 2026
Large depth-of-focus via programmable space-time light sheets
The diffraction-free property of space-time wave packets has led to an abundance of interest in the field of optical physics. This feature may also find utility in applications for biomedical optics. Specifically, the programmability of the space-time light sheet can yield µm-thick light sheets with widths that resist diffraction in free space over several millimeters, whereas similarly sized Airy, Bessel, or Gaussian light sheets diverge significantly when focused to reach comparable widths. Here, we experimentally and numerically demonstrate this, and confirm that a 10-µm-thick space-time light sheet, achieved without a focusing lens and synthesized by tuning the spectral tilt angle of the light cone, maintains its width over a free-space propagation distance of 2 mm. In comparison, we find that over the same propagation distance, the Airy, Bessel, and Gaussian light sheets, all with starting thicknesses of ∼10 µm, become ∼4.5× to ∼10× wider, respectively. Space-time light sheets thus offer an opportunity for significantly extended depth-of-focus for light sheet microscopy.
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- Award ID(s):
- 2426040
- PAR ID:
- 10574968
- Publisher / Repository:
- Optical Society of America
- Date Published:
- Journal Name:
- Optics Letters
- Volume:
- 50
- Issue:
- 6
- ISSN:
- 0146-9592; OPLEDP
- Format(s):
- Medium: X Size: Article No. 1795
- Size(s):
- Article No. 1795
- Sponsoring Org:
- National Science Foundation
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