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This content will become publicly available on June 9, 2026

Title: A long term time lapse microscopy technique for Arabidopsis roots
Time lapse microscopy is a transformative technique for plant cell and developmental biology. Light sheet microscopy, which manipulates the amount of light a sample is exposed to in order to minimize phototoxicity and maximize signal intensity, is an increasingly popular tool for time lapse imaging. However, many light sheet imaging systems are not designed with the unique properties of plant samples in mind. Recent advances have decreased the cost and increased the technical accessibility of light sheet microscopy, but plant samples still require special preparation to be compatible with these new systems. Here, we apply a novel light sheet microscopy system to regenerating Arabidopsis roots damaged via laser ablation. To adapt this system for Arabidopsis roots we establish a new protocol for sample mounting, as well as an automated root tip tracking system that requires no additional proprietary software. The methods presented here can be used to increase researcher access to long-term time-lapse imaging in Arabidopsis biology.  more » « less
Award ID(s):
2109634
PAR ID:
10603931
Author(s) / Creator(s):
; ;
Publisher / Repository:
Frontiers in Plant Science
Date Published:
Journal Name:
Frontiers in Plant Science
Volume:
16
ISSN:
1664-462X
Format(s):
Medium: X
Sponsoring Org:
National Science Foundation
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