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Autotrophic and heterotrophic microbes in stream biofilms dominate biogeochemical cycling and rely on nutrient and energy resources for growth and productivity. In the boreal forest, variation in these resources can originate from permafrost distribution and controls competition for nutrients between stream autotrophs and heterotrophs. We investigated which resources control nutrient uptake and metabolism in headwater stream biofilms of subarctic Alaska, USA, and how resource availability affects competition for inorganic nutrients. We hypothesized that the competitive outcome between autotrophs and heterotrophs for inorganic nutrients would be dependent on availability of organic C, or inorganic nutrients (N and P). To test our hypotheses, we measured resource limitation at the patch and reach scales along a permafrost gradient in interior Alaska. At the patch scale, nutrient diffusing substrata revealed that, secondary to light, N and P were colimiting to autotrophic growth, whereas C was primarily limiting to heterotrophic respiration. In the presence of labile C, heterotrophs exhibited a larger response to nutrient enrichment and outcompeted autotrophs for inorganic nutrients. At the reach scale, light availability had the largest influence on nutrient uptake, but inorganic nutrients were also important. The positive response to increased nutrient and C availability at the patch scale suggests that the predicted increase in exports into fluvial networks with permafrost degradation will alter biofilm structure and function. Ultimately, biofilm communities will shift to more heterotroph-dominated patches if heterotrophs outcompete autotrophs for inorganic nutrients. As permafrost thaws and nutrients and organic C mobilize into streams, nutrient uptake dynamics and competition within biofilms will be altered, affecting nutrient use and export.
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Nutrient Limitation Induces a Productivity Decline From Light‐Controlled Maximum
Abstract Nutrient impacts on productivity in stream ecosystems can be obscured by light limitation imposed by canopy cover and water turbidity, thereby creating uncertainties in linking nutrient and productivity regimes. Evaluations of nutrient limitations are often based on a response ratio (RR) quantifying productivity stimulation above ambient levels given augmented nutrient supply. This metric neglects the primacy of light effects on productivity. We propose an alternative approach to quantify nutrient limitations using a “decline ratio” (DR), which quantifies the productivity decline from the maximum established by light availability. The DR treats light as the first‐order control and nutrient depletion as a disturbance causing productivity decline, allowing separation of nutrient and light influences. We used DR to assess nutrient diffusing substrate (NDS) experiments with three nutrients (nitrogen [N], phosphorus [P], iron [Fe]) from five Greenland streams during summer, where light is not limited due to the lack of canopy and low turbidity. We tested two hypotheses: (a) productivity maximum (i.e., highest chlorophyll‐aamong NDS treatments) is controlled by light and (b) DR depends on both light and nutrients. The productivity maximum was strongly predicted by light (R2 = 0.60). The productivity decline induced by N limitation (i.e., DRN) was best explained by light availability when parameterized with either dissolved inorganic nitrogen concentration (R2 = 0.79) or N:Fe ratio (R2 = 0.87). These predictions outperformed predictions of RR for which light was not a significant factor. Reversing the perspective on nutrient limitation from “stimulation above ambient” to “decline below maximum” provides insights into both light and nutrient impacts on stream productivity.
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- Award ID(s):
- 2000649
- PAR ID:
- 10616840
- Publisher / Repository:
- American Geophysical Union
- Date Published:
- Journal Name:
- Journal of Geophysical Research: Biogeosciences
- Volume:
- 130
- Issue:
- 4
- ISSN:
- 2169-8953
- Format(s):
- Medium: X
- Sponsoring Org:
- National Science Foundation
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- Free Publicly Accessible Full Text
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Accepted Manuscript
- Journal Article:
- https://doi.org/10.1029/2024JG008597
