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This content will become publicly available on March 1, 2026

Title: Tau and MAP6 establish labile and stable domains on microtubules
We previously documented that individual microtubules in the axons of cultured juvenile rodent neurons consist of a labile domain and a stable domain and that experimental depletion of tau results in selective shortening and partial stabilization of the labile domain. After first confirming these findings in adult axons, we sought to understand the mechanism that accounts for the formation and maintenance of these microtubule domains. We found that fluorescent tau and MAP6 ectopically expressed in RFL-6 fibroblasts predominantly segregate on different microtubules or different domains on the same microtubule, with the tau-rich ones becoming more labile than in control cells and the MAP6-rich ones being more stable than in control cells. These and other experimental findings, which we studied further using computational modeling with tunable parameters, indicate that these two MAPs do not merely bind to pre-existing stable and labile domains but actually create stable and labile domains on microtubules.  more » « less
Award ID(s):
1915477
PAR ID:
10656083
Author(s) / Creator(s):
; ; ; ; ; ; ;
Publisher / Repository:
iScience
Date Published:
Journal Name:
iScience
Volume:
28
Issue:
3
ISSN:
2589-0042
Page Range / eLocation ID:
111785
Format(s):
Medium: X
Sponsoring Org:
National Science Foundation
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