Search for: All records

Abstract This study investigates the causes of shifts in the subsiding edge of the boreal winter Hadley cell (HC) in response to a comprehensive treatment of ocean surface albedo (OSA) in the fully coupled CESM2. The focus is on an in‐depth understanding of the atmospheric dynamical processes that influence the HC subsiding edge. Two sets of experiments were performed: one utilizing the default OSA, and the other employing the comprehensive OSA that accounts for realistic physical mechanisms. The results show that implementing the comprehensive OSA simulates an El Niño‐like warming pattern in reference to the default experiment, which leads to an HC contraction. Examination of zonal mean momentum dynamics in the upper troposphere reveals that variations in meridional winds, crucial for determining the HC extent, are primarily driven by the differences in the horizontal eddy momentum flux derivative. The findings indicate that the equatorward shift in meridional temperature gradients enhances subtropical zonal winds and baroclinicity along their equatorial flanks, amplifying equatorward‐propagating Rossby waves. This, in turn, alters the eddy momentum flux, reshaping the pattern of the derivatives of horizontal eddy momentum flux, constraining meridional winds, and resulting in the equatorward movement of the HC subsiding edge. A scaling theory further supports the results of the HC contraction, showing that the increased subtropical zonal winds and the equatorward shift of the Intertropical Convergence Zone (ITCZ) elevate the atmospheric angular momentum and eventually limit the expansion of the HC. 

ABSTRACT Recombinant adeno associated virus (rAAV) vectors have become popular delivery vehicles for in vivo gene therapies, but demand for rAAVs continues to outpace supply. Platform processes for rAAV production are being developed by many manufacturers, and transient chemical transfection of human embryonic kidney 293 (HEK293) cells is currently the most popular approach. However, the cutting edge nature of rAAV process development encourages manufacturers to keep cell culture media formulations, plasmid sequences, and other details proprietary, which creates hurdles for small companies and academic labs seeking to innovate in this space. To address this problem, we leveraged the resources of an academic‐industry consortium (Advanced Mammalian Biomanufacturing Innovation Center, AMBIC) to develop an rAAV production system based on transient transfection of suspension HEK293 cells adapted to an in‐house, chemically defined medium. We found that balancing iron and calcium levels in the medium were crucial for maintaining transfection efficiency and minimizing cell aggregation, respectively. A design of experiments approach was used to optimize the transient transfection process for batch rAAV production, and PEI:DNA ratio and cell density at transfection were the parameters with the strongest effects on vector genome (VG) titer. When the optimized transient process was transferred between two university sites, VG titers were within a twofold range. Analytical characterization showed that purified rAAV from the AMBIC process had comparable viral protein molecular weights versus vector derived from commercial processes, but differences in transducing unit (TU) titer were observed between vector preps. The developed media formulation, transient transfection process, and analytics for VG titer, capsid identity, and TU titer constitute a set of workflows that can be adopted by others to study fundamental problems that could improve product yield and quality in the nascent field of rAAV manufacturing. 

The Antarctic ozone “hole” was discovered in 1985, and man-made ozone- depleting substances (ODS) are its primary cause. Following reductions of ODSs under the Montreal Protocol, signs of ozone recovery have been reported, based largely on observations and broad yet compelling model-data comparisons. While such approaches are highly valuable, they don't provide rigorous statistical detection of the temporal and spatial structure of Antarctic ozone recovery in the presence of internal climate variability. Here, we apply pattern-based detection and attribution methods as employed in climate change studies to separate anthropogenically forced ozone responses from internal variability, relying on trend pattern information as a function of month and height. The analysis uses satellite observations together with single-model and multi-model ensemble simulations to identify and quantify the month-height Antarctic ozone recovery “fingerprint”. We demonstrate that the data and simulations show remarkable agreement in the fingerprint pattern of the ozone response to decreasing ODSs since 2005. We also show that ODS forcing has enhanced ozone internal variability during the austral spring, influencing detection of forced responses and their time of emergence. Our results provide robust statistical and physical evidence that actions taken under the Montreal Protocol to reduce ODSs are indeed resulting in the beginning of Antarctic ozone recovery, defined as increases in ozone consistent with expected month-height patterns. 

Abstract Atmospheric rivers (ARs), intrusions of warm and moist air, can effectively drive weather extremes over the Arctic and trigger subsequent impact on sea ice and climate. What controls the observed multi-decadal Arctic AR trends remains unclear. Here, using multiple sources of observations and model experiments, we find that, contrary to the uniform positive trend in climate simulations, the observed Arctic AR frequency increases by twice as much over the Atlantic sector compared to the Pacific sector in 1981-2021. This discrepancy can be reconciled by the observed positive-to-negative phase shift of Interdecadal Pacific Oscillation (IPO) and the negative-to-positive phase shift of Atlantic Multidecadal Oscillation (AMO), which increase and reduce Arctic ARs over the Atlantic and Pacific sectors, respectively. Removing the influence of the IPO and AMO can reduce the projection uncertainties in near-future Arctic AR trends by about 24%, which is important for constraining projection of Arctic warming and the timing of an ice-free Arctic. 

Abstract Water vapor and cirrus clouds in the tropical tropopause layer (TTL) are important for the climate and are largely controlled by temperature in the TTL. On interannual timescales, both stratospheric and tropospheric modes of the large‐scale variability could affect temperatures in the TTL. Here multiple linear regression (MLR) is used to investigate explained variance in the cold point tropopause temperature (CPT), cold point tropopause height (CPZ), 83 hPa water vapor (WV83), 83 hPa ozone (O₃83), and total cirrus cloud fraction with cloud base (TTLCCF) and top (ALLCF) above 14.5 km, all averaged over 15°S‐15°N. Predictors of the MLR are a set of stratospheric and tropospheric large‐scale modes of variability. The MLR explains significant variance in CPT (76%), CPZ (78%), WV83 (65%), O₃83 (62%), TTLCCF (52%), and ALLCF (36%). The interannual variability of CPT and WV83 is dominated by stratospheric processes associated with the Quasi‐Biennial Oscillation (QBO) and Brewer‐Dobson Circulation (BDC), whereas the variability of CPZ, O₃83, TTLCCF and ALLCF is also controlled by 500 hPa temperature (T500). Residual variability in CPT and CPZ not captured by the MLR are further significantly correlated to stratospheric temperature. It is shown that the portion of the BDC's shallow branch missed by the eddy heat flux based BDC index contributes significant amounts of the explained variances.