Search for: All records

This study presents an advanced multi-view drone swarm imaging system for the three-dimensional characterization of smoke plume dispersion dynamics. The system comprises a manager drone and four worker drones, each equipped with high-resolution cameras and precise GPS modules. The manager drone uses image feedback to autonomously detect and position itself above the plume, then commands the worker drones to orbit the area in a synchronized circular flight pattern, capturing multi-angle images. The camera poses of these images are first estimated, then the images are grouped in batches and processed using Neural Radiance Fields (NeRF) to generate high-resolution 3D reconstructions of plume dynamics over time. Field tests demonstrated the system's ability to capture critical plume characteristics including volume dynamics, wind-driven directional shifts, and lofting behavior at a temporal resolution of about 1 s. The 3D reconstructions generated by this system provide unique field data for enhancing the predictive models of smoke plume dispersion and fire spread. Broadly, the drone swarm system offer a versatile platform for high resolution measurements of pollutant emissions and transport in wildfires, volcanic eruptions, prescribed burns, and industrial processes, ultimately supporting more effective fire control decisions and mitigating wildfire risks. 

Bacterial cells alter gene expression in response to changes in population density in a process called quorum sensing (QS). In Vibrio harveyi, LuxO, a low cell density activator of sigma factor-54 (RpoN), is required for transcription of five non-coding regulatory sRNAs, Qrr1-Qrr5, which each repress translation of the master QS regulator LuxR. Vibrio parahaemolyticus, the leading cause of bacterial seafood-borne gastroenteritis, also contains five Qrr sRNAs that control OpaR (the LuxR homolog), controlling capsule polysaccharide (CPS), motility, and metabolism. We show that in a Δ luxO deletion mutant, opaR was de-repressed and CPS and biofilm were produced. However, in a Δ rpoN mutant, opaR was repressed, no CPS was produced, and less biofilm production was observed compared to wild type. To determine why opaR was repressed, expression analysis in Δ luxO showed all five qrr genes were repressed, while in Δ rpoN the qrr2 gene was significantly de-repressed. Reporter assays and mutant analysis showed Qrr2 sRNA can act alone to control OpaR. Bioinformatics analysis identified a sigma-70 (RpoD) -35 -10 promoter overlapping the canonical sigma-54 (RpoN) -24 -12 promoter in the qrr2 regulatory region. The qrr2 sigma-70 promoter element was also present in additional Vibrio species indicating it is widespread. Mutagenesis of the sigma-70 -10 promoter site in the Δ rpoN mutant background, resulted in repression of qrr2. Analysis of qrr quadruple deletion mutants, in which only a single qrr gene is present, showed that only Qrr2 sRNA can act independently to regulate opaR . Mutant and expression data also demonstrated that RpoN and the global regulator, Fis, act additively to repress qrr2 . Our data has uncovered a new mechanism of qrr expression and shows that Qrr2 sRNA is sufficient for OpaR regulation. Importance The quorum sensing non-coding sRNAs are present in all Vibrio species but vary in number and regulatory roles among species. In the Harveyi clade, all species contain five qrr genes, and in V. harveyi these are transcribed by sigma-54 and are additive in function. In the Cholerae clade, four qrr genes are present, and in V. cholerae the qrr genes are redundant in function. In V. parahaemolyticus , qrr2 is controlled by two overlapping promoters. In an rpoN mutant, qrr2 is transcribed from a sigma-70 promoter that is present in all V. parahaemolyticus strains and in other species of the Harveyi clade suggesting a conserved mechanism of regulation. Qrr2 sRNA can function as the sole Qrr sRNA to control OpaR. 

A multiline ring anchor (MRA) system has been developed as a cost-effective alternative for securing arrays of floating offshore wind turbines (FOWTs) to the seabed. Multiline attachments can improve the economically competitiveness of FOWTs by reducing the capital cost of the support system for the floating structures. FOWTs can be subjected to severe wind and wave conditions resulting in extreme loads to the anchor system. Thus, the reliable design of the anchor system requires proper determination of the extreme mooring line loads acting on the anchor needed to secure FOWTs to the seabed. Previous studies showed the MRA in soft clay has clear advantages over existing anchors under the extreme horizontal loading conditions imposed by catenary moorings; however, its performance relative to conventional anchors under extreme vertical loading imposed by taut mooring systems requires further investigation. This study presents predictions of extreme loads on floating structures secured by taut mooring systems and evaluates the potential for developing an economical anchor for resisting these extreme loads. 

Photosynthetic O 2 evolution is catalyzed by the Mn 4 CaO 5 cluster of the water oxidation complex of the photosystem II (PSII) complex. The photooxidative self-assembly of the Mn 4 CaO 5 cluster, termed photoactivation, utilizes the same highly oxidizing species that drive the water oxidation in order to drive the incorporation of Mn 2+ into the high-valence Mn 4 CaO 5 cluster. This multistep process proceeds with low quantum efficiency, involves a molecular rearrangement between light-activated steps, and is prone to photoinactivation and misassembly. A sensitive polarographic technique was used to track the assembly process under flash illumination as a function of the constituent Mn 2+ and Ca 2+ ions in genetically engineered membranes of the cyanobacterium Synechocystis sp. PCC6803 to elucidate the action of Ca 2+ and peripheral proteins. We show that the protein scaffolding organizing this process is allosterically modulated by the assembly protein Psb27, which together with Ca 2+ stabilizes the intermediates of photoactivation, a feature especially evident at long intervals between photoactivating flashes. The results indicate three critical metal-binding sites: two Mn and one Ca, with occupation of the Ca site by Ca 2+ critical for the suppression of photoinactivation. The long-observed competition between Mn 2+ and Ca 2+ occurs at the second Mn site, and its occupation by competing Ca 2+ slows the rearrangement. The relatively low overall quantum efficiency of photoactivation is explained by the requirement of correct occupancy of these metal-binding sites coupled to a slow restructuring of the protein ligation environment, which are jointly necessary for the photooxidative trapping of the first stable assembly intermediate. 

The trend of offshore wind energy in deeper water that is expected to shift from fixed to floating platforms requires a cost-effective anchor solution for floating offshore wind turbines (FOWTs). Multiline ring anchor (MRA) has been developed as a cost-effective solution for FOWTs due to its capability of anchoring multiple mooring lines, its high efficiency, and its availability to a wide range of soils and loading conditions. While previous preliminary studies on the anchor performance provide useful insights on how the potential advantages of the MRA can improve load capacity, these studies are limited to focusing on optimizing the anchor design in certain soil and loading conditions. By contrast, the MRA will be installed in seabeds under more complex conditions that depend on geological location, water depth of at-place, and environmental conditions, of which wind, current, and wave are major components. These may result in additional substantial extra capital costs, delays in the projects, and safety issues, when the complex conditions are not properly considered. Specifically, the installation time and expenses of the offshore anchor are very susceptible to anchor types, installation methods, and environmental conditions. For this reason, this paper compares two existing offshore anchor installation methods and different anchor types on the basis of their performance under the same severe environmental condition. In evaluating the installability of the MRA, this paper conducts a comparative scenario study. The results show that the anchor installations and anchor handling vessel (AHV) operations are sensitive to weather conditions and AHV sizes. In view of total weather standby, the results show that anchor types or installation methods have little effect on it due to their relatively shorter duration than other work sequences. However, the MRA can benefit in substantially reducing transport time and costs due to its compact size. The MRA can be more efficient and cost-effective than other alternatives under complex and severe weather conditions. 

A<sc>bstract</sc> A search for beyond-the-standard-model neutral Higgs bosons decaying to a pair of bottom quarks, and produced in association with at least one additional bottom quark, is performed with the CMS detector. The data were recorded in proton-proton collisions at a centre-of-mass energy of 13 TeV at the CERN LHC and correspond to an integrated luminosity of 36.7–126.9 fb⁻¹, depending on the probed mass range. No signal above the standard model background expectation is observed. Upper limits on the production cross section times branching fraction are set for Higgs bosons in the mass range of 125–1800 GeV. The results are interpreted in benchmark scenarios of the minimal supersymmetric standard model, as well as suitable classes of two-Higgs-doublet models. 

This report presents a comprehensive collection of searches for new physics performed by the ATLAS Collaboration during the Run~2 period of data taking at the Large Hadron Collider, from 2015 to 2018, corresponding to about 140~$$^{-1}$$ of $$\sqrt{s}=13$$~TeV proton--proton collision data. These searches cover a variety of beyond-the-standard model topics such as dark matter candidates, new vector bosons, hidden-sector particles, leptoquarks, or vector-like quarks, among others. Searches for supersymmetric particles or extended Higgs sectors are explicitly excluded as these are the subject of separate reports by the Collaboration. For each topic, the most relevant searches are described, focusing on their importance and sensitivity and, when appropriate, highlighting the experimental techniques employed. In addition to the description of each analysis, complementary searches are compared, and the overall sensitivity of the ATLAS experiment to each type of new physics is discussed. Summary plots and statistical combinations of multiple searches are included whenever possible.