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  1. Studies of cell-extracellular matrix (ECM) interactions within fibrous systems such as collagen or fibrin are challenging, particularly if peri-cellular stiffness cannot be monitored. Here we present our light-based method for non-invasive patterning of molecular crosslinking combined with multi-axes optical tweezers active microrheology to map ECM stiffness landscapes. This method allows us to generate prescribed stiffness gradients and associated anisotropies, which model stiffness of the natural peri-cellular ECM. Patterned crosslinking induces strain hardening and measured stiffness gradients are in agreement with predicted strain fields. Migratory cells respond to these gradients as assessed by change in F-actin distribution and morphological properties.
  2. Aims. An interplanetary coronal mass ejection (ICME) event was observed by the Solar Orbiter at 0.8 AU on 2020 April 19 and by Wind at 1 AU on 2020 April 20. Futhermore, an interplanetary shock wave was driven in front of the ICME. Here, we focus on the transmission of the magnetic fluctuations across the shock and we analyze the characteristic wave modes of solar wind turbulence in the vicinity of the shock observed by both spacecraft. Methods. The observed ICME event is characterized by a magnetic helicity-based technique. The ICME-driven shock normal was determined by magnetic coplanarity method for the Solar Orbiter and using a mixed plasma and field approach for Wind. The power spectra of magnetic field fluctuations were generated by applying both a fast Fourier transform and Morlet wavelet analysis. To understand the nature of waves observed near the shock, we used the normalized magnetic helicity as a diagnostic parameter. The wavelet-reconstructed magnetic field fluctuation hodograms were used to further study the polarization properties of waves. Results. We find that the ICME-driven shock observed by Solar Orbiter and Wind is a fast, forward oblique shock with a more perpendicular shock angle at the Wind position. After themore »shock crossing, the magnetic field fluctuation power increases. Most of the magnetic field fluctuation power resides in the transverse fluctuations. In the vicinity of the shock, both spacecraft observe right-hand polarized waves in the spacecraft frame. The upstream wave signatures fall within a relatively broad and low frequency band, which might be attributed to low frequency MHD waves excited by the streaming particles. For the downstream magnetic wave activity, we find oblique kinetic Alfvén waves with frequencies near the proton cyclotron frequency in the spacecraft frame. The frequency of the downstream waves increases by a factor of ∼7–10 due to the shock compression and the Doppler effect.« less
  3. Context. Aims. We systematically search for magnetic flux rope structures in the solar wind to within the closest distance to the Sun of ~0.13 AU, using data from the third and fourth orbits of the Parker Solar Probe. Methods. We extended our previous magnetic helicity-based technique of identifying magnetic flux rope structures. The method was improved upon to incorporate the azimuthal flow, which becomes larger as the spacecraft approaches the Sun. Results. A total of 21 and 34 magnetic flux ropes are identified during the third (21-day period) and fourth (17-day period) orbits of the Parker Solar Probe, respectively. We provide a statistical analysis of the identified structures, including their relation to the streamer belt and heliospheric current sheet crossing.