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Abstract IntroductionIn order to be positioned to address the increasing strain of burnout and worsening nurse shortage, a better understanding of factors that contribute to nursing workload is required. This study aims to examine the difference between order‐based and clinically perceived nursing workloads and to quantify factors that contribute to a higher clinically perceived workload. DesignA retrospective cohort study was used on an observational dataset. MethodsWe combined patient flow, nurse staffing and assignment, and workload intensity data and used multivariate linear regression to analyze how various shift, patient, and nurse‐level factors, beyond order‐based workload, affect nurses' clinically perceived workload. ResultsAmong 53% of our samples, the clinically perceived workload is higher than the order‐based workload. Factors associated with a higher clinically perceived workload include weekend or night shifts, shifts with a higher census, patients within the first 24 h of admission, and male patients. ConclusionsThe order‐based workload measures tended to underestimate nurses' clinically perceived workload. We identified and quantified factors that contribute to a higher clinically perceived workload, discussed the potential mechanisms as to how these factors affect the clinically perceived workload, and proposed targeted interventions to better manage nursing workload. Clinical RelevanceBy identifying factors associated with a high clinically perceived workload, the nurse manager can provide appropriate interventions to lighten nursing workload, which may further reduce the risk of nurse burnout and shortage. 

Abstract Cell plate formation during cytokinesis entails multiple stages occurring concurrently and requiring orchestrated vesicle delivery, membrane remodelling, and timely deposition of polysaccharides, such as callose. Understanding such a dynamic process requires dissection in time and space; this has been a major hurdle in studying cytokinesis. Using lattice light sheet microscopy (LLSM), we studied cell plate development in four dimensions, through the behavior of yellow fluorescent protein (YFP)-tagged cytokinesis-specific GTPase RABA2a vesicles. We monitored the entire duration of cell plate development, from its first emergence, with the aid of YFP–RABA2a, in both the presence and absence of cytokinetic callose. By developing a robust cytokinetic vesicle volume analysis pipeline, we identified distinct behavioral patterns, allowing the identification of three easily trackable cell plate developmental phases. Notably, the phase transition between phase I and phase II is striking, indicating a switch from membrane accumulation to the recycling of excess membrane material. We interrogated the role of callose using pharmacological inhibition with LLSM and electron microscopy. Loss of callose inhibited the phase transitions, establishing the critical role and timing of the polysaccharide deposition in cell plate expansion and maturation. This study exemplifies the power of combining LLSM with quantitative analysis to decode and untangle such a complex process. 

Compact laser plasma accelerators generate high-energy electron beams with increasing quality. When used in inverse Compton backscattering, however, the relatively large electron energy spread jeopardizes potential applications requiring small bandwidths. We present here a novel interaction scheme that allows us to compensate for the negative effects of the electron energy spread on the spectrum, by introducing a transverse spatial frequency modulation in the laser pulse. Such a laser chirp, together with a properly dispersed electron beam, can substantially reduce the broadening of the Compton bandwidth due to the electron energy spread. We show theoretical analysis and numerical simulations for hard X-ray Thomson sources based on laser plasma accelerators.