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The nucleus is at the nexus of mechanotransduction and the final barrier for most first line chemotherapeutics. Here, we study the intersection between nuclear-cytoskeletal coupling and chemotherapy nuclear internalization. We find that chronic and acute modulation of intracellular filaments changes nuclear influx of doxorubicin (DOX). Rapid changes in cell strain by disruption of cytoskeletal and nuclear filaments sensitize nuclei to DOX, whereas chronic reduction of cell strain desensitize nuclei to DOX. Extracted nuclei from invasive cancer cells lines from different tissues have distinct nuclear permeability to DOX. Last, we show that mechano-priming of cells by paclitaxel markedly improves DOX nuclear internalization, rationalizing the observed drug synergies. Our findings reveal that nuclear uptake is a critical, previously unquantified aspect of drug resistance. With nuclear permeability to chemotherapy being tunable via modulation of nuclear mechanotransduction, mechano-priming may be useful to help overcome drug resistance in the future. 

The Center for High Angular Resolution Astronomy (CHARA) Array is a six-element interferometer with baselines ranging from 34 to 331 m. Three new beam combiners are entering operation: MYSTIC is a 6-telescope combiner for K-band; SPICA is a 6-telescope combiner for the visible R-band; and SILMARIL is a 3-telescope combiner for high sensitivity in H and K-bands. A seventh, portable telescope will use fiber optics for beam transport and will increase the baselines to 1 km. Observing time is available through a program funded by NSF. The programs are solicited and peer-reviewed by NSF’s National Optical Infrared Astronomy Research Laboratory. The open community access has significantly expanded the range of astronomical investigations of stars and their environments. Here we summarize the scientific work and the on-going technical advances of the CHARA Array. 

ABSTRACT The kinematic morphology–density relation of galaxies is normally attributed to a changing distribution of galaxy stellar masses with the local environment. However, earlier studies were largely focused on slow rotators; the dynamical properties of the overall population in relation to environment have received less attention. We use the SAMI Galaxy Survey to investigate the dynamical properties of ∼1800 early and late-type galaxies with log (M⋆/M⊙) > 9.5 as a function of mean environmental overdensity (Σ5) and their rank within a group or cluster. By classifying galaxies into fast and slow rotators, at fixed stellar mass above log (M⋆/M⊙) > 10.5, we detect a higher fraction (∼3.4σ) of slow rotators for group and cluster centrals and satellites as compared to isolated-central galaxies. We find similar results when using Σ5 as a tracer for environment. Focusing on the fast-rotator population, we also detect a significant correlation between galaxy kinematics and their stellar mass as well as the environment they are in. Specifically, by using inclination-corrected or intrinsic $$\lambda _{R_{\rm {e}}}$$ values, we find that, at fixed mass, satellite galaxies on average have the lowest $$\lambda _{\, R_{\rm {e}},\rm {intr}}$$, isolated-central galaxies have the highest $$\lambda _{\, R_{\rm {e}},\rm {intr}}$$, and group and cluster centrals lie in between. Similarly, galaxies in high-density environments have lower mean $$\lambda _{\, R_{\rm {e}},\rm {intr}}$$ values as compared to galaxies at low environmental density. However, at fixed Σ5, the mean $$\lambda _{\, R_{\rm {e}},\rm {intr}}$$ differences for low and high-mass galaxies are of similar magnitude as when varying Σ5 ($$\Delta \lambda _{\, R_{\rm {e}},\rm {intr}} \sim 0.05$$, with σrandom = 0.025, and σsyst < 0.03). Our results demonstrate that after stellar mass, environment plays a significant role in the creation of slow rotators, while for fast rotators we also detect an independent, albeit smaller, impact of mass and environment on their kinematic properties. 

ABSTRACT We measure the gas-phase metallicity gradients of 248 galaxies selected from Data Release 2 of the SAMI Galaxy Survey. We demonstrate that there are large systematic discrepancies between the metallicity gradients derived using common strong emission line metallicity diagnostics. We determine which pairs of diagnostics have Spearman’s rank coefficients greater than 0.6 and provide linear conversions to allow the accurate comparison of metallicity gradients derived using different strong emission line diagnostics. For galaxies within the mass range 8.5 < log (M/M⊙) < 11.0, we find discrepancies of up to 0.11 dex/Re between seven popular diagnostics in the metallicity gradient–mass relation. We find a suggestion of a break in the metallicity gradient–mass relation, where the slope shifts from negative to positive, occurs between 9.5 < log (M/M⊙) < 10.5 for the seven chosen diagnostics. Applying our conversions to the metallicity gradient–mass relation, we reduce the maximum dispersion from 0.11 dex/Re to 0.02 dex/Re. These conversions provide the most accurate method of converting metallicity gradients when key emission lines are unavailable. We find that diagnostics that share common sets of emission line ratios agree best, and that diagnostics calibrated through the electron temperature provide more consistent results compared to those calibrated through photoionization models. 

Abstract We presentH-band interferometric observations of the red supergiant (RSG) AZ Cyg that were made with the Michigan Infra-Red Combiner (MIRC) at the six-telescope Center for High Angular Resolution Astronomy (CHARA) Array. The observations span 5 yr (2011–2016), which offers insight into the short and long-term evolution of surface features on RSGs. Using a spectrum of AZ Cyg obtained with SpeX on the NASA InfraRed Telescope Facility (IRTF) and synthetic spectra calculated from spherical MARCS, spherical PHOENIX, and SAtlas model atmospheres, we deriveT_effis between 3972 K and 4000 K and $\log g$between −0.50 and 0.00, depending on the stellar model used. Using fits to the squared visibility and GAIA parallaxes, we measure its average radius $R={911}_{-50}^{+57}{R}_{\odot }$. Reconstructions of the stellar surface using our model-independent imaging codes SQUEEZE and OITOOLS.jl show a complex surface with small bright features that appear to vary on a timescale of less than one year and larger features that persist for more than one year. The 1D power spectra of these images suggest a characteristic size of 0.52–0.69R_⋆for the larger, long lived features. This is close to the values of 0.51–0.53R_⋆that are derived from 3D RHD models of stellar surfaces. We conclude that interferometric imaging of this star is in line with predictions of 3D RHD models but that short-term imaging is needed to more stringently test predictions of convection in RSGs. 

ABSTRACT We have entered a new era where integral-field spectroscopic surveys of galaxies are sufficiently large to adequately sample large-scale structure over a cosmologically significant volume. This was the primary design goal of the SAMI Galaxy Survey. Here, in Data Release 3, we release data for the full sample of 3068 unique galaxies observed. This includes the SAMI cluster sample of 888 unique galaxies for the first time. For each galaxy, there are two primary spectral cubes covering the blue (370–570 nm) and red (630–740 nm) optical wavelength ranges at spectral resolving power of R = 1808 and 4304, respectively. For each primary cube, we also provide three spatially binned spectral cubes and a set of standardized aperture spectra. For each galaxy, we include complete 2D maps from parametrized fitting to the emission-line and absorption-line spectral data. These maps provide information on the gas ionization and kinematics, stellar kinematics and populations, and more. All data are available online through Australian Astronomical Optics Data Central.