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Direct numerical simulation (DNS) of turbulent flows is computationally expensive and cannot be applied to flows with large Reynolds numbers. Low-resolution large eddy simulation (LES) is a popular alternative, but it is unable to capture all of the scales of turbulent transport accurately. Reconstructing DNS from low-resolution LES is critical for large-scale simulation in many scientific and engineering disciplines, but it poses many challenges to existing super-resolution methods due to the complexity of turbulent flows and computational cost of generating frequent LES data. We propose a physics-guided neural network for reconstructing frequent DNS from sparse LES data by enhancing its spatial resolution and temporal frequency. Our proposed method consists of a partial differential equation (PDE)-based recurrent unit for capturing underlying temporal processes and a physics-guided super-resolution model that incorporates additional physical constraints. We demonstrate the effectiveness of both components in reconstructing the Taylor-Green Vortex using sparse LES data. Moreover, we show that the proposed recurrent unit can preserve the physical characteristics of turbulent flows by leveraging the physical relationships in the Navier-Stokes equation.