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Abstract Understanding and forecasting the geoeffectiveness of a coronal mass ejection (CME) is crucial for protecting infrastructure in the near-Earth space environment and on Earth. In this study, we present a novel fusion model to forecast the geoeffectiveness of CME events. Our model combines convolutional neural networks for feature learning and a prediction network for feature fusion and event classification. The model is trained by observations from instruments including the Large Angle Spectroscopic Coronagraph (LASCO) on board the Solar and Heliospheric Observatory (SOHO) and the Atmospheric Imaging Assembly (AIA) and Helioseismic and Magnetic Imager (HMI) on board the Solar Dynamics Observatory (SDO). The trained model is then used to predict whether an Earth-reaching CME will cause a geomagnetic storm and/or the probability that the CME will cause such a storm. Experimental results based on a five-fold cross validation scheme demonstrate the good performance of our fusion model, achieving a mean true skill statistic (TSS) score of 0.703 when the model is used as a deterministic prediction tool, and a mean Brier score of 0.095 when the model is used as a probabilistic forecasting tool, where a TSS score of 1 or a Brier score of 0 indicates perfect performance. This work contributes to forecasting the causal relationship between Earth-directed CMEs and geomagnetic storms in solar-terrestrial interactions. 

Context.High-resolution magnetograms are crucial for studying solar flare dynamics because they enable the precise tracking of magnetic structures and rapid field changes. The Helioseismic and Magnetic Imager on board the Solar Dynamics Observatory (SDO/HMI) has been an essential provider of vector magnetograms. However, the spatial resolution of the HMI magnetograms is limited and hence is not able to capture the fine structures that are essential for understanding flare precursors. The Near InfraRed Imaging Spectropolarimeter on the 1.6 m Goode Solar Telescope (GST/NIRIS) at Big Bear Solar Observatory (BBSO) provides a better spatial resolution and is therefore more suitable to track the fine magnetic features and their connection to flare precursors. Aims.We propose DeepHMI, a machine-learning method for solar image super-resolution, to enhance the transverse and line-of-sight magnetograms of solar active regions (ARs) collected by SDO/HMI to better capture the fine-scale magnetic structures that are crucial for understanding solar flare dynamics. The enhanced HMI magnetograms can also be used to study spicules, sunspot light bridges and magnetic outbreaks, for which high-resolution data are essential. Methods.DeepHMI employs a conditional diffusion model that is trained using ground-truth images obtained by an inversion analysis of Stokes measurements collected by GST/NIRIS. Results.Our experiments show that DeepHMI performs better than the commonly used bicubic interpolation method in terms of four evaluation metrics. In addition, we demonstrate the ability of DeepHMI through a case study of the enhancement of SDO/HMI transverse and line-of-sight magnetograms of AR 12371 to GST/NIRIS data. 

Abstract We present a novel deep generative model, named GenMDI, to improve the temporal resolution of line-of-sight (LOS) magnetograms of solar active regions (ARs) collected by the Michelson Doppler Imager (MDI) on board the Solar and Heliospheric Observatory. Unlike previous studies that focus primarily on spatial super-resolution of MDI magnetograms, our approach can perform temporal super-resolution, which generates and inserts synthetic data between observed MDI magnetograms, thus providing finer temporal structure and enhanced details in the LOS data. The GenMDI model employs a conditional diffusion process, which synthesizes images by considering both preceding and subsequent magnetograms, ensuring that the generated images are not only of high quality but also temporally coherent with the surrounding data. Experimental results show that the GenMDI model performs better than the traditional linear interpolation method, especially in ARs with dynamic evolution in magnetic fields. 

Koley et al. indicate that fatty acid oxidation occurs concomitantly with fatty acid biosynthesis in multiple plant tissues, including seeds that are thought to stably house storage reserves. This study suggests that some lipid breakdown and fatty acid oxidation is the rule and not the exception in plant metabolism. 

In eukaryotic organisms, protein kinases regulate diverse protein activities and signaling pathways through phosphorylation of specific protein substrates. Isolating and characterizing kinase substrates is vital for defining downstream signaling pathways. The Kinase Client (KiC) assay is an in vitro synthetic peptide LC-MS/MS phosphorylation assay that has enabled identification of protein substrates (i.e., clients) for various protein kinases. For example, previous use of a 2,100-member (2k) peptide library identified substrates for the extracellular ATP receptor-like kinase, P2K1. Many P2K1 clients were confirmed by additional in vitro and in planta studies, including Integrin-Linked Kinase 4 (ILK4), for which we provide the evidence herein. In addition, we developed a new KiC peptide library containing 8,000 (8k) peptides based on phosphorylation sites primarily from Arabidopsis thaliana datasets. The 8k peptides are enriched for sites with conservation in other angiosperm plants, with the paired goals of representing functionally conserved sites and usefulness for screening kinases from diverse plants. Screening the 8k library with the active P2K1 kinase domain identified 177 phosphopeptides, including calcineurin B-like protein (CBL9) and G protein alpha subunit 1 (GPA1), which functions in cellular calcium signaling. We confirmed that P2K1 directly phosphorylates CBL9 and GPA1 through in vitro kinase assays. This expanded 8k KiC assay will be a useful tool for identifying novel substrates across diverse plant protein kinases, ultimately facilitating the exploration of previously undiscovered signaling pathways. 

Plants are remarkable in their ability to adapt to changing environments, with receptor-like kinases (RLKs) playing a pivotal role in perceiving and transmitting environmental cues into cellular responses. Despite extensive research on RLKs from the plant kingdom, the function and activity of many kinases, i.e., their substrates or “clients”, remain uncharted. To validate a novel client prediction workflow and learn more about an important RLK, this study focuses on P2K1 (DORN1), which acts as a receptor for extracellular ATP (eATP), playing a crucial role in plant stress resistance and immunity. We designed a Kinase-Client (KiC) assay library of 225 synthetic peptides, incorporating previously identified P2K phosphorylated peptides and novel predictions from a deep-learning phosphorylation site prediction model (MUsite) and a trained hidden Markov model (HMM) based tool, HMMER. Screening the library against purified P2K1 cytosolic domain (CD), we identified 46 putative substrates, including 34 novel clients, 27 of which may be novel peptides, not previously identified experimentally. Gene Ontology (GO) analysis among phosphopeptide candidates revealed proteins associated with important biological processes in metabolism, structure development, and response to stress, as well as molecular functions of kinase activity, catalytic activity, and transferase activity. We offer selection criteria for efficient furtherin vivoexperiments to confirm these discoveries. This approach not only expands our knowledge of P2K1’s substrates and functions but also highlights effective prediction algorithms for identifying additional potential substrates. Overall, the results support use of the KiC assay as a valuable tool in unraveling the complexities of plant phosphorylation and provide a foundation for predicting the phosphorylation landscape of plant species based on peptide library results.