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Short-chain fatty acylations establish connections between cell metabolism and regulatory pathways. Lysine acetoacetylation (Kacac) was recently identified as a new histone mark. However, regulatory elements, substrate proteins, and epigenetic functions of Kacac are not yet fully understood, hindering further in-depth understanding of acetoacetate-modulated (patho)physiological processes. Here, we created a chemo-immunological approach for reliable detection of Kacac, and demonstrated that acetoacetate serves as the primary precursor for histone Kacac. We report the enzymatic addition of the Kacac mark by the acyltransferases GCN5, p300, and PCAF, and its removal by the deacetylase HDAC3. Furthermore, we establish acetoacetyl-CoA synthetase as a key regulator of cellular Kacac levels. A comprehensive proteomic analysis has identified 139 Kacac sites on 85 human proteins. Bioinformatics analysis of Kacac substrates and RNA sequencing data reveal the broad impacts of Kacac on multifaceted cellular processes. These findings unveil pivotal regulatory mechanisms for the acetoacetate-mediated Kacac pathway, opening a new avenue for further investigation into ketone body functions in various pathophysiological states. 

Methacrylyl-CoA is a key metabolic intermediate in the valine catabolic pathway. Its accumulation has been found to be cytotoxic and associated with pathological conditions. Nevertheless, detailed biological effects of methacrylyl-CoA and methacrylate in human physiology and pathology are poorly understood. We propose that the electrophilicity of the alkene bond in the methacrylyl group can react with the cysteine residues in proteins resulting in an unexplored protein post-translational modification (PTM), cysteine S-2-carboxypropylation (C2cp). To test and validate this mechanistic hypothesis, we experimentally detected and profiled S-2-carboxypropylated proteins from the complex cellular proteome with the design and application of a bioorthogonal chemical probe, N-propargyl methacrylamide. We tested the probe in different mammalian cell models and demonstrated its versatility and sensitivity to protein cysteine S-2-carboxypropylation. We established quantitative chemical proteomics for global and site-specific profiling of protein S-2-carboxypropylation, which successfully identified 403 S-2-carboxypropylated proteins and 120 cysteine modification sites from HEK293T cells. Through bioinformatic analysis, we found that C2cp-modified proteins were involved in a variety of critical cellular functions including translation, RNA splicing, and protein folding. Our chemoproteomic studies demonstrating the proteome-wide distribution of cysteine S-2-carboxypropylation provide a new biochemical mechanism for the functional investigation of methacrylyl-CoA and understanding valine-related metabolic disorders. 

This paper presents a new turbo decision feedback equalizer and decoder (TDFED) for the orthogonal time-frequency space (OTFS) system of underwater mobile acoustic communications where the communication channel suffers from severe multipath and Doppler effects simultaneously. The proposed TDFED employs a set of feedforward and feedback filters in the time domain instead of the common approach that employs a normalized least mean square equalizer in the delay-Doppler domain. The receiver also utilizes low-complexity improved proportionate normalized least mean square channel estimation in the delay-Doppler domain. Practical OTFS modulation schemes are designed for acoustic transmission at a center frequency of 115 kHz and a symbol rate of 11.5 ksps (kilo-symbols-per-second). Several lake experiments in mobile communication scenarios are conducted to evaluate the proposed OTFS in comparison to the single-carrier coherent modulation (SCCM) and the orthogonal frequency division modulation (OFDM) schemes. The experimental results demonstrate that the proposed OTFS receiver effectively reduces the accuracy requirements of the Doppler compensation algorithm compared to the SCCM and OFDM schemes. The proposed TDFED algorithm achieves a much better bit error rate against long-multipath fading and severe Doppler shift than the existing delay-Doppler domain equalizers. 

A low-cost testing procedure is proposed to measure the orientation and range of an acoustic array transmitter at the bottom of the lake or ocean in relation to a surface receiver. Since the GPS measurements are available only on the surface, an inertial measurement unit (IMU) and a depth sensor are used on the transmitter to track the orientation and depth when the transmitter platform is lowered to the deployment position underwater. Procedures for post-experiment data processing are detailed to combine the GPS observations and IMU measurements which yield the locations of the surface receiver relative to the bottom transmitter. Using GPS corrections and long-term stable correction of the IMU sensor, the proposed procedure achieves meter-level accuracy of the localization measurement. 

This paper investigates the impact of mobility on underwater acoustic communication networks in which the propagation delay is comparable to or larger than the packet duration. An underwater acoustic wireless network, consisting of static and mobile nodes, is studied for its link-layer channel utilization. Synchronous and asynchronous media access control (MAC) protocols are employed with ALOHA, TDMA (time-division multiple access), and artificial intelligence (AI) agent nodes. The simulation results of a multi-node network show that the asynchronous MAC protocols achieve up to 6.66× higher channel utilization than synchronous protocols by allowing time slots to be shorter than the maximum propagation delay among nodes and permitting asynchronous transmission time. The high mobility of a few mobile nodes also favors asynchronous protocols and increases the overall channel utilization. However, node mobility causes more difficulties for the AI node to learn the environment, which may be ineffective to achieve higher gains in channel utilization.