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Collaboration is an important learning process. During collaborative learning, students engage in group activities where they converge on goals, solve problems and make joint decisions. To understand the process of collaboration, we focused on how behavior and interaction patterns contribute to the social-relational space of collaboration. We have designed a multilayered conceptual model for the collaboration process and an observation rubric that identifies behaviors and interactions during collaboration that serves as the foundation for machine learning models that can provide behavioral insight into the process of collaboration. This study reports results on several validation studies performed to establish a validation argument for our collaboration conceptual model and collaboration rubric. Through disconfirming evidence, interrater reliability testing, expert reviews, and focus group interviews, we found that our stratified architecture of collaboration and rubric provide valid accounts and descriptions of human behavior and interactions that can be used to substantiate the collaboration process.