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  1. Abstract

    Multidimensional photography can capture optical fields beyond the capability of conventional image sensors that measure only two-dimensional (2D) spatial distribution of light. By mapping a high-dimensional datacube of incident light onto a 2D image sensor, multidimensional photography resolves the scene along with other information dimensions, such as wavelength and time. However, the application of current multidimensional imagers is fundamentally restricted by their static optical architectures and measurement schemes—the mapping relation between the light datacube voxels and image sensor pixels is fixed. To overcome this limitation, we propose tunable multidimensional photography through active optical mapping. A high-resolution spatial light modulator, referred to as an active optical mapper, permutes and maps the light datacube voxels onto sensor pixels in an arbitrary and programmed manner. The resultant system can readily adapt the acquisition scheme to the scene, thereby maximising the measurement flexibility. Through active optical mapping, we demonstrate our approach in two niche implementations: hyperspectral imaging and ultrafast imaging.

  2. We present high-resolution, high-speed fluorescence lifetime imaging microscopy (FLIM) of live cells based on a compressed sensing scheme. By leveraging the compressibility of biological scenes in a specific domain, we simultaneously record the time-lapse fluorescence decay upon pulsed laser excitation within a large field of view. The resultant system, referred to as compressed FLIM, can acquire a widefield fluorescence lifetime image within a single camera exposure, eliminating the motion artifact and minimizing the photobleaching and phototoxicity. The imaging speed, limited only by the readout speed of the camera, is up to 100 Hz. We demonstrated the utility of compressed FLIM in imaging various transient dynamics at the microscopic scale.

  3. Compressed ultrafast photography (CUP) is a computational optical imaging technique that can capture transient dynamics at an unprecedented speed. Currently, the image reconstruction of CUP relies on iterative algorithms, which are time-consuming and often yield nonoptimal image quality. To solve this problem, we develop a deep-learning-based method for CUP reconstruction that substantially improves the image quality and reconstruction speed. A key innovation toward efficient deep learning reconstruction of a large three-dimensional (3D) event datacube (x,y,t) (x,y, spatial coordinate;t, time) is that we decompose the original datacube into massively parallel two-dimensional (2D) imaging subproblems, which are much simpler to solve by a deep neural network. We validated our approach on simulated and experimental data.