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Title: Correlation of Atomic Force Microscopy Tapping Forces to Mechanical Properties of Lipid Membranes
There is considerable interest in measuring, with nanoscale spatial resolution, the physical properties of lipid membranes because of their role in the physiology of living systems. Due to its ability to nondestructively image surfaces in solution, tapping mode atomic force microscopy (TMAFM) has proven to be a useful technique for imaging lipid membranes. However, further information concerning the mechanical properties of surfaces is contained within the time-resolved tip/sample force interactions. The tapping forces can be recovered by taking the second derivative of the cantilever deflection signal and scaling by the effective mass of the cantilever; this technique is referred to as scanning probe acceleration microscopy. Herein, we describe how the maximum and minimum tapping forces change with surface mechanical properties. Furthermore, we demonstrate how these changes can be used to measure mechanical changes in lipid membranes containing cholesterol.  more » « less
Award ID(s):
1054211
PAR ID:
10018319
Author(s) / Creator(s):
; ; ; ;
Date Published:
Journal Name:
ASME 2012 International Design Engineering Technical Conferences and Computers and Information in Engineering Conference
Volume:
5
Page Range / eLocation ID:
303-310
Format(s):
Medium: X
Sponsoring Org:
National Science Foundation
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