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Title: Fast Magic‐Angle Spinning 19 F NMR Spectroscopy of HIV‐1 Capsid Protein Assemblies
Abstract 19F NMR spectroscopy is an attractive and growing area of research with broad applications in biochemistry, chemical biology, medicinal chemistry, and materials science. We have explored fast magic angle spinning (MAS)19F solid‐state NMR spectroscopy in assemblies of HIV‐1 capsid protein. Tryptophan residues with fluorine substitution at the 5‐position of the indole ring were used as the reporters. The19F chemical shifts for the five tryptophan residues are distinct, reflecting differences in their local environment. Spin‐diffusion and radio‐frequency‐driven‐recoupling experiments were performed at MAS frequencies of 35 kHz and 40–60 kHz, respectively. Fast MAS frequencies of 40–60 kHz are essential for consistently establishing19F–19F correlations, yielding interatomic distances of the order of 20 Å. Our results demonstrate the potential of fast MAS19F NMR spectroscopy for structural analysis in large biological assemblies.  more » « less
Award ID(s):
1708773
PAR ID:
10077571
Author(s) / Creator(s):
 ;  ;  ;  ;  ;  ;  ;  ;  ;  
Publisher / Repository:
Wiley Blackwell (John Wiley & Sons)
Date Published:
Journal Name:
Angewandte Chemie International Edition
Volume:
57
Issue:
50
ISSN:
1433-7851
Page Range / eLocation ID:
p. 16375-16379
Format(s):
Medium: X
Sponsoring Org:
National Science Foundation
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