Microbially Induced Calcite Precipitation (MICP), or bio-cementation, is a promising bio-mediated technology that can improve the engineering properties of soils through the precipitation of calcium carbonate. Despite significant advances in the technology, concerns regarding the fate of produced NH4+by-products have remained largely unaddressed. In this study, five 3.7-meter long soil columns each containing one of three different soils were improved using ureolytic bio-cementation, and post-treatment NH4+by-product removal was investigated during the application of 525 L of a high pH and high ionic strength rinse solution. During rinsing, reductions in aqueous NH4+were observed in all columns from initial concentrations between ≈100 mM to 500 mM to final values between ≈0.3 mM and 20 mM with higher NH4+concentrations observed at distances furthest from the injection well. In addition, soil Vsmeasurements completed during rinse injections suggested that no significant changes in cementation integrity occurred during NH4+removal. After rinsing and a 12 hour stop flow period, all column solutions achieved cumulative NH4+removals exceeding 97.9%. Soil samples collected following rinsing, however, contained significant sorbed NH4+masses that appeared to have a near linear relationship with surrounding aqueous NH4+concentrations. While these results suggest that NH4+can be successfully removed from bio-cemented soils, acceptable limits for NH4+aqueous concentrations and sorbed NH4+masses will likely bemore »
Specimens of silica sand treated via enzyme induced carbonate precipitation (EICP) showed surprisingly high strength at a relatively low carbonate content when non-fat powdered milk was included in the treatment solution. EICP is a biologically-based soil improvement technique that uses free urease enzyme to catalyze the hydrolysis of urea in an aqueous solution, producing carbonate ions and alkalinity that in the presence of calcium cations leads to precipitation of calcium carbonate. The strength achieved at less than 1.4% carbonate content via a single cycle of treatment was unprecedented compared to results reported in the literature from both EICP and microbially induced carbonate precipitation (MICP). Scanning electron microscope images show that in the specimens treated with the solution containing powdered milk the carbonate precipitate was concentrated at interparticle contacts. The impact of these results include reductions in the concentration of substrate and enzyme required to achieve a target compressive strength, reduction in the undesirable ammonium chloride by-product, and, depending on the desired strength, reduction in the number of cycles of EICP treatment. These advantages enhance the potential for development of a sustainable method of soil improvement via hydrolysis of urea.
- Publication Date:
- NSF-PAR ID:
- 10153351
- Journal Name:
- Scientific Reports
- Volume:
- 9
- Issue:
- 1
- ISSN:
- 2045-2322
- Publisher:
- Nature Publishing Group
- Sponsoring Org:
- National Science Foundation
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Abstract -
Numerous laboratory studies in the past decade have demonstrated the ability of microbially induced calcite precipitation (MICP), a bio-mediated soil improvement method, to favorably transform a soil’s engineering properties including increased shear strength and stiffness with reductions in hydraulic conductivity and porosity. Despite significant advances in treatment application techniques and characterization of post-treatment engineering properties, relationships between biogeochemical conditions during precipitation and post-treatment material properties have remained poorly understood. Bacterial augmentation, stimulation, and cementation treatments can vary dramatically in their chemical constituents, concentrations, and ratios between researchers, with specific formulas oftentimes perpetuating despite limited understanding of their engineering implications. In this study, small-scale batch experiments were used to systematically investigate how biogeochemical conditions during precipitate synthesis may influence resulting bio-cementation and related material engineering behaviors. Aqueous solution chemistry was monitored in time to better understand the relationship between the kinetics of ureolysis and calcium carbonate precipitation, and resulting precipitates. Following all experiments, precipitates were evaluated using x-ray diffraction and scanning electron microscopy to characterize mineralogy and morphology. Results obtained from these investigations are expected to help identify the primary chemical and biological factors during synthesis that may control bio-cementation material properties and
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The end goal of this research is assessing the feasibility of using enzyme induced carbonate precipitation (EICP) to create a cemented top layer to control runoff erosion in sloping sandy soil. The paper presents the results of an experimental study of bench-scale tests on EICP-treated sands to determine a treatment method feasible for field placement for this application. The soils tested were two natural sands and Ottawa 20-30 sand used as control. The EICP application methods were percolation by gravity, one-step mix-compact, and two-step mix-compact. Other conditions considered were pre-rinsing the sand prior to treatment, adjusting soil pH prior to treatment, and changing the EICP solution concentration. Promising results for this field application were obtained using the two-step mix-compact when the soil was first mixed with the urease enzyme solution before compaction. Considering that the EICP reaction starts once all components are added, this method would ensure that the reaction does not take place before the protective layer of treated soil has been installed. The effect of pre-rinsing the natural sand was not consistent throughout the testing conditions and its role in improving soil cementation in natural sand needs further study.
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Microbially induced desaturation and precipitation (MIDP) via denitrification has the potential to reduce earthquake-induced liquefaction potential by two mechanisms: calcium carbonate precipitation to mechanically strengthen soil and biogenic gas production to desaturate and dampen pore pressure changes in soil. Lab-scale tests have demonstrated effective desaturation and improved mechanical strength by MIDP. However, in laboratory tests, gas pockets and lenses form causing upheaval as a result of low overburden pressures. The characteristics of biogenic gas formation, distribution, and retention need to be evaluated to gain comprehensive understanding of the effectiveness of this treatment at depth before and after an earthquake event. MIDP treatment during centrifuge loading conditions is being performed to simulate field stress conditions, prior to complete process scale-up for field application. A simplified numerical model was developed to evaluate the scaling effects on biogenic gas generation between the centrifuge model and prototype scale. The results indicate that diffusion of soluble N2 is negligible at both the model and prototype scales for the simulated reaction rate. However, the simplified model did not consider other pore-scale influences and mixing from liquid-gas transfer and transport. Future modeling work will need to add these features.
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Abstract Microbially-induced calcium carbonate precipitation (MICP) is a bio-cementation process that can improve the engineering properties of granular soils through the precipitation of calcium carbonate (CaCO3) minerals on soil particle surfaces and contacts. The technology has advanced rapidly as an environmentally conscious soil improvement method, however, our understanding of the effect of changes in field-representative environmental conditions on the physical and chemical properties of resulting precipitates has remained limited. An improved understanding of the effect of subsurface geochemical and soil conditions on process reaction kinetics and the morphology and mineralogy of bio-cementation may be critical towards enabling successful field-scale deployment of the technology and improving our understanding of the long-term chemical permanence of bio-cemented soils in different environments. In this study, thirty-five batch experiments were performed to specifically investigate the influence of seawater ions and varying soil materials on the mineralogy, morphology, and reaction kinetics of ureolytic bio-cementation. During experiments, differences in reaction kinetics were quantified to identify conditions inhibiting CaCO3precipitation and ureolysis. Following experiments, scanning electron microscopy, x-ray diffraction, and chemical composition analyses were employed to quantify differences in mineralogical compositions and material morphology. Ions present in seawater and variations in soil materials were shown to significantly influence ureolyticmore »
