An official website of the United States government
The genome inside the eukaryotic cells is guarded by a unique shell structure, called the nuclear envelope (NE), made of lipid membranes. This structure has an ultra torus topology with thousands of torus-shaped holes that imparts the structure a high flexural stiffness. Inspired from this biological design, here we present a novel “torene” architecture to design lightweight shell structures with ultra-stiffness for engineering applications. We perform finite element analyses on classic benchmark problems to investigate the mechanics of torene shells. This study reveals that the torene shells can achieve one order of magnitude or higher flexural stiffness than traditional shells with the same amount of material. This novel geometric strategy opens new avenues to exploit additive manufacturing to design lightweight shell structures for extreme mechanical environments.
more »
« less
Geometry of the nuclear envelope determines its flexural stiffness
During closed mitosis in fission yeast, growing microtubules push onto the nuclear envelope to deform it, which results in fission into two daughter nuclei. The resistance of the envelope to bending, quantified by the flexural stiffness, helps determine the microtubule-dependent nuclear shape transformations. Computational models of envelope mechanics have assumed values of the flexural stiffness of the envelope based on simple scaling arguments. The validity of these estimates is in doubt, however, owing to the complex structure of the nuclear envelope. Here, we performed computational analysis of the bending of the nuclear envelope under applied force using a model that accounts for envelope geometry. Our calculations show that the effective bending modulus of the nuclear envelope is an order of magnitude larger than a single membrane and approximately five times greater than the nuclear lamina. This large bending modulus is in part due to the 45 nm separation between the two membranes, which supports larger bending moments in the structure. Further, the effective bending modulus is highly sensitive to the geometry of the nuclear envelope, ranging from twofold to an order magnitude larger than the corresponding single membrane. These results suggest that spatial variations in geometry and mechanical environment of the envelope may cause a spatial distribution of flexural stiffness in the same nucleus. Overall, our calculations support the possibility that the nuclear envelope may balance significant mechanical stresses in yeast and in cells from higher organisms.
more »
« less
- PAR ID:
- 10188445
- Date Published:
- Journal Name:
- Molecular Biology of the Cell
- Volume:
- 31
- Issue:
- 16
- ISSN:
- 1059-1524
- Page Range / eLocation ID:
- 1815 to 1821
- Format(s):
- Medium: X
- Sponsoring Org:
- National Science Foundation
More Like this
-
-
ABSTRACT The fission yeastSchizosaccharomyces pombedivides via closed mitosis, meaning that spindle elongation and chromosome segregation transpire entirely within the closed nuclear envelope. Both the spindle and nuclear envelope must undergo shape changes and exert varying forces on each other during this process. Previous work has demonstrated that nuclear envelope expansion (Yam, He, Zhang, Chiam, & Oliferenko, 2011; Mori & Oliferenko, 2020) and spindle pole body (SPB) embedding in the nuclear envelope are required for normalS. pombemitosis, and mechanical modeling has described potential contributions of the spindle to nuclear morphology (Fang et al., 2020; Zhu et al., 2016). However, it is not yet fully clear how and to what extent the nuclear envelope and mitotic spindle each directly shape each other during closed mitosis. Here, we investigate this relationship by observing the behaviors of spindles and nuclei in live mitotic fission yeast following laser ablation. First, we characterize these dynamics in mitoticS. pombenuclei with increased envelope tension, finding that nuclear envelope tension can both bend the spindle and slow elongation. Next, we directly probe the mechanical connection between spindles and nuclear envelopes by ablating each structure. We demonstrate that envelope tension can be relieved by severing spindles and that spindle compression can be relieved by rupturing the envelope. We interpret our experimental data via two quantitative models that demonstrate that fission yeast spindles and nuclear envelopes are a mechanical pair that can each shape the other's morphology.more » « less
-
null (Ed.)Flexural and thermomechanical properties of the epoxy-based carbon fiber composites (CFCs) on addition of single and binary nanoparticles (nanoclay and graphene) have been investigated. It was found that nanoclay acts more effectively in increasing the stiffness of the CFCs, whereas graphene is more effective in achieving higher strength. Nanoclay-added samples exhibited highest flexural (64.5 GPa) and storage (25.3 GPa) modulus among all types. Graphene-added samples showed highest improvement (by 21%) in flexural strength and exhibited most stable thermomechanical properties with highest energy dissipation capability (3.1 GPa loss modulus) in flexural test and dynamic mechanical analysis (DMA), respectively. By contrast, addition of binary nanoparticles reduced the stiffness and significantly increased the strain to failure (42%) of the composites. Optical microscopy and scanning electron microscopy indicated that addition of nanoparticles significantly reduced delamination and matrix cracking of the CFCs because of strong interfacial bonding and toughened matrix, respectively.more » « less
-
Abstract Objective. The force that an electrocorticography (ECoG) array exerts on the brain manifests when it bends to match the curvature of the skull and cerebral cortex. This force can negatively impact both short-term and long-term patient outcomes. Here we provide a mechanical characterization of a novel liquid crystal polymer (LCP) ECoG array prototype to demonstrate that its thinner geometry reduces the force potentially applied to the cortex of the brain. Approach. We built a low-force flexural testing machine to measure ECoG array bending forces, calculate their effective flexural moduli, and approximate the maximum force they could exerted on the human brain. Main results. The LCP ECoG prototype was found to have a maximal force less than 20% that of any commercially available ECoG arrays that were tested. However, as a material, LCP was measured to be as much as 24× more rigid than silicone, which is traditionally used in ECoG arrays. This suggests that the lower maximal force resulted from the prototype’s thinner profile (2.9×–3.25×). Significance. While decreasing material stiffness can lower the force an ECoG array exhibits, our LCP ECoG array prototype demonstrated that flexible circuit manufacturing techniques can also lower these forces by decreasing ECoG array thickness. Flexural tests of ECoG arrays are necessary to accurately assess these forces, as material properties for polymers and laminates are often scale dependent. As the polymers used are anisotropic, elastic modulus cannot be used to predict ECoG flexural behavior. Accounting for these factors, we used our four-point flexure testing procedure to quantify the forces exerted on the brain by ECoG array bending. With this experimental method, ECoG arrays can be designed to minimize force exerted on the brain, potentially improving both acute and chronic clinical utility.more » « less
-
A biphasic trajectory for maize stalk mechanics shaped by genetic, environmental, and biotic factorsSUMMARY Stalk mechanical properties impact plant stability and interactions with pathogenic microorganisms. The evaluation of stalk mechanics has focused primarily on the end‐of‐season outcomes and defined differences among inbred and hybrid maize genotypes. However, there is a gap in understanding how these different end‐of‐season outcomes are achieved. This study measured stalk flexural stiffness in maize inbred genotypes across multiple environments and in maize commercial hybrid genotypes under different disease states. Under all conditions, stalk flexural stiffness followed a biphasic trajectory, characterized by a linear increase phase and a sustained phase. Within a genotype, the environment or disease state altered the rate of increase in the linear phase but did not impact the timing of transition to the sustained phase. Whereas between genotypes, the timing of transition between phases varied. Destructive 3‐point bend tests of inbred stalks showed that the trajectory of stalk mechanics is defined by the bending modulus, not the geometry. Together, these results define a biphasic trajectory of maize stalk mechanics that can be modulated by internal and external factors. This work provides a foundation for breeding programs to make informed decisions when selecting for optimized stalk mechanical trajectories, which are necessary for enhancing resilience to environmental stresses.more » « less
- Free Publicly Accessible Full Text
-
Accepted Manuscript1.0
- Journal Article:
- https://doi.org/10.1091/mbc.E20-02-0163
