skip to main content
US FlagAn official website of the United States government
dot gov icon
Official websites use .gov
A .gov website belongs to an official government organization in the United States.
https lock icon
Secure .gov websites use HTTPS
A lock ( lock ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

Attention:

The NSF Public Access Repository (PAR) system and access will be unavailable from 10:00 PM ET on Thursday, February 12 until 1:00 AM ET on Friday, February 13 due to maintenance. We apologize for the inconvenience.


Title: Projection method as a probe for multiplexing/demultiplexing of magnetically enriched biological tissues
The unmet demand for cheap, accurate, and fast multiplexing of biomarkers has urged nanobiotechnology to prioritize the invention of new biomarkers that make feasible the remote detection, identification, and quantification of biological units, such as regenerative tissues. Here, we introduce a novel approach that highlights magnetic nanowires (MNWs) with such capabilities. This method employs the stable magnetization states of MNWs as a unique characteristic that can be realized by projecting the MNWs' switching field on the backward field ( P Hb ), also known as the irreversible switching field. Experimentally, several types of MNWs were directly synthesized inside polycarbonate tissues and their P Hb characteristics were measured and analyzed. Our results show that the P Hb gives an excellent identification and quantification characteristic for demultiplexing MNWs embedded in these tissues. Furthermore, this method significantly improves the characterization speed by a factor of 50×–100× that makes it superior to the current state of the art that ceased the progression of magnetic nanoparticles in multiplexing/demultiplexing applications.  more » « less
Award ID(s):
1642268
PAR ID:
10209704
Author(s) / Creator(s):
;
Date Published:
Journal Name:
RSC Advances
Volume:
10
Issue:
22
ISSN:
2046-2069
Page Range / eLocation ID:
13286 to 13292
Format(s):
Medium: X
Sponsoring Org:
National Science Foundation
More Like this
  1. Magnetic properties such as coercivity, remanence and saturation magnetization will determine the area enclosed by the hysteresis loop of a magnetic material, which also represents magnetic heating. Nanowarming of cryopreserved organs is a new application for magnetic heating using nanoparticles. In this paper, isolated Ni MNW of different sizes and shapes are studied via micromagnetic simulation to explore the optimization of heating using individual MNW. Ellipsoidal MNWs with small (30nm) diameters turn out to be most promising in heating ability due to their large hysteresis area and their potential to distribute uniformly in an organ that is being heated. In addition to optimized heating, a special switching pattern of magnetic moment was also observed for cylindrical large (200nm) MNW. This special switching pattern can trigger applications such as quantum computing. 
    more » « less
  2. null (Ed.)
    Nuclear Magnetic Resonance (NMR) spectroscopy is a quantitative analytical tool commonly utilized for metabolomics analysis. Quantitative NMR (qNMR) is a field of NMR spectroscopy dedicated to the measurement of analytes through signal intensity and its linear relationship with analyte concentration. Metabolomics-based NMR exploits this quantitative relationship to identify and measure biomarkers within complex biological samples such as serum, plasma, and urine. In this review of quantitative NMR-based metabolomics, the advancements and limitations of current techniques for metabolite quantification will be evaluated as well as the applications of qNMR in biomedical metabolomics. While qNMR is limited by sensitivity and dynamic range, the simple method development, minimal sample derivatization, and the simultaneous qualitative and quantitative information provide a unique landscape for biomedical metabolomics, which is not available to other techniques. Furthermore, the non-destructive nature of NMR-based metabolomics allows for multidimensional analysis of biomarkers that facilitates unambiguous assignment and quantification of metabolites in complex biofluids. 
    more » « less
  3. Abstract Terahertz (THz) communication is an up‐and‐coming technology for the sixth‐generation wireless network. The realization of ultra‐high‐speed THz communication requires the combination of multi‐dimensional multiplexing schemes, including polarization division multiplexing (PDM), mode division multiplexing (MDM), and wavelength division multiplexing, to increase channel capacity. However, most existing devices for MDM in the THz regime are single‐purpose and incapable of multi‐dimensional modulation. Here, all‐dielectric metasurfaces are designed for 2D multiplexing/demultiplexing, which takes the lead in combining orbital angular momentum (OAM) MDM and PDM in the THz regime. The multi‐functional wavefront phase modulations and interleaved meta‐atom arrangements are used to realize polarization‐selective multichannel OAM mode (de)multiplexing, in which the linear‐polarized 4‐channel and circular‐polarized 6‐channel demultiplexing are experimentally demonstrated. Between different linear‐polarized channels, the measured maximum crosstalk is −16.88 dB, and the isolation of each channel can be greater than 10 dB in a range wider than 0.1 THz. This study paves the way for multi‐dimensional multiplexing in the THz regime, which may benefit extremely high‐capacity and integrated THz communication systems. The proposed design strategy is readily applied to multi‐functional metasurfaces for microwaves and far infrared light, facilitating the development of multiplexing technology and OAM‐related applications. 
    more » « less
  4. Roux, Simon (Ed.)
    ABSTRACT Oxford Nanopore Technologies provides multiplexing options for DNA and cDNA sequencing, but not for direct RNA sequencing. Here we describe a duplexing approach and validate it by simultaneously sequencing theSaccharomyces cerevisiaerRNA from wild type and knockout that have differential rRNA modifications, successfully demultiplexing the data using bioinformatics approaches. 
    more » « less
  5. null (Ed.)
    Isolating and analyzing tumor-derived exosomes (TEX) can provide important information about the state of a tumor, facilitating early diagnosis and prognosis. Since current isolation methods are mostly laborious and expensive, we propose herein a fast and cost-effective method based on a magnetic nanoplatform to isolate TEX. In this work, we have tested our method using three magnetic nanostructures: (i) Ni magnetic nanowires (MNWs) (1500 × 40 nm), (ii) Fe3O4 nanorods (NRs) (41 × 7 nm), and (iii) Fe3O4 cube-octahedral magnetosomes (MGs) (45 nm) obtained from magnetotactic bacteria. The magnetic response of these nanostructures has been characterized, and we have followed their internalization inside canine osteosarcoma OSCA-8 cells. An overall depiction has been obtained using a combination of Fluorescence and Scanning Electron Microscopies. In addition, Transmission Electron Microscopy images have shown that the nanostructures, with different signs of degradation, ended up being incorporated in endosomal compartments inside the cells. Small intra-endosomal vesicles that could be precursors for TEX have also been identified. Finally, TEX have been isolated using our magnetic isolation method and analyzed with a Nanoparticle tracking analyzer (NanoSight). We observed that the amount and purity of TEX isolated magnetically with MNWs was higher than with NRs and MGs, and they were close to the results obtained using conventional non-magnetic isolation methods. 
    more » « less