Rapidly changing economics, customer preferences, and policy to address climate change and local environmental pollutants have driven increased deployment of a wide range of distributed energy resources in the U.S. electricity system. Distributed energy resources have enabled an expanded role for energy consumers and non-utility third parties to reshape system costs, drawing renewed attention to the potential of reforming electricity rate design based on the further application of cost-causal principals to improve overall system fairness and efficiency. One mechanism to move toward greater application of cost-causal rate design is dynamic pricing, which varies electricity prices across time and location to reflect costs of providing electricity to consumers under specific market conditions and grid operation conditions. While dynamic electricity pricing has penetrated some markets, and it has not been widely implemented, particularly for residential consumers. In this review article, we provide a brief summary of electricity rate design, including the possibility of introducing dynamic prices, and explain why dynamic prices are more reflective of the short-run marginal costs of electricity supply than volumetric rates. We then explore the barriers to the widespread adoption of residential dynamic pricing, emphasizing technical, economic, and political challenges. Our assessment reflects the ability of dynamicmore »
Designing a Compact, Low-Cost FRET Microscopy Platform for the Undergraduate Classroom
ABSTRACT Advances in fluorescent biosensors allow researchers to spatiotemporally monitor a diversity of biochemical reactions and secondary messengers. However, commercial microscopes for the specific application of Förster Resonance Energy Transfer (FRET) are prohibitively expensive to implement in the undergraduate classroom, owing primarily to the dynamic range required and need for ratiometric emission imaging. The purpose of this article is to provide a workflow to design a low-cost, FRET-enabled microscope and to equip the reader with sufficient knowledge to compare commercial light sources, optics, and cameras to modify the device for a specific application. We used this approach to construct a microscope that was assembled by undergraduate students with no prior microscopy experience that is suitable for most single-cell cyan and yellow fluorescent protein FRET applications. The utility of this design was demonstrated by measuring small metabolic oscillations by using a lactate FRET sensor expressed in primary mouse pancreatic islets, highlighting the biologically suitable signal-to-noise ratio and dynamic range of our compact microscope. The instructions in this article provide an effective teaching tool for undergraduate educators and students interested in implementing FRET in a cost-effective manner.
- Award ID(s):
- 1845801
- Publication Date:
- NSF-PAR ID:
- 10217198
- Journal Name:
- The Biophysicist
- Volume:
- 1
- Issue:
- 2
- ISSN:
- 2578-6970
- Sponsoring Org:
- National Science Foundation
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