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Deep Brain Stimulation (DBS) surgeries are not new, although they were only granted approval in the U.S. by the U.S. Food and Drug Administration (FDA) in 2002 for advanced Parkinson’s Disease (PD). In 2016, DBS surgery was approved for earlier stages of PD. This does not mean that DBS surgery, generally considered minimally invasive, does not come without commensurate risks. The Mayo Clinic identifies DBS as a serious and potential risky procedure, whereby those eligible must carefully weigh pros and cons. The aim of this paper is to provide a general overview of deep brain stimulation surgery and to present the findings of available informational resources on 14 hospital and medical center web sites that were reviewed, pertaining to surgical procedures and policies: pre-operative to post-operative. The article focuses on critiquing available educational DBS materials and their adequacy in addressing potential risks of DBS surgery. The findings indicate that hospital informational resources on the DBS surgical technique reaffirm each other’s educational materials and that they positively inform patient decision-making. These factors can be linked to better post-operative recovery. However, the materials provided by the hospitals overemphasize the positive aspects of DBS with relatively little detail about potential side effects. This article also outlines the potential short-term and long-term side effects of DBS surgery as identified by the DBS educational literature found on the hospital web sites reviewed.
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Minimally invasive brain injections for viral-mediated transgenesis: New tools for behavioral genetics in sticklebacks
Behavioral genetics in non-model organisms is currently gated by technological limitations. However, with the growing availability of genome editing and functional genomic tools, complex behavioral traits such as social behavior can now be explored in diverse organisms. Here we present a minimally invasive neurosurgical procedure for a classic behavioral, ecological and evolutionary system: threespine stickleback ( Gasterosteus aculeatus ). Direct brain injection enables viral-mediated transgenesis and pharmaceutical delivery which bypasses the blood-brain barrier. This method is flexible, fast, and amenable to statistically powerful within-subject experimental designs, making it well-suited for use in genetically diverse animals such as those collected from natural populations. Developing this minimally invasive neurosurgical protocol required 1) refining the anesthesia process, 2) building a custom surgical rig, and 3) determining the normal recovery pattern allowing us to clearly identify warning signs of failure to thrive. Our custom-built surgical rig (publicly available) and optimized anesthetization methods resulted in high (90%) survival rates and quick behavioral recovery. Using this method, we detected changes in aggression from the overexpression of either of two different genes, arginine vasopressin ( AVP ) and monoamine oxidase ( MAOA ), in outbred animals in less than one month. We successfully used multiple promoters to drive expression, allowing for tailored expression profiles through time. In addition, we demonstrate that widely available mammalian plasmids work with this method, lowering the barrier of entry to the technique. By using repeated measures of behavior on the same fish before and after transfection, we were able to drastically reduce the necessary sample size needed to detect significant changes in behavior, making this a viable approach for examining genetic mechanisms underlying complex social behaviors.
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- Award ID(s):
- 1915134
- PAR ID:
- 10251016
- Editor(s):
- Fugmann, Sebastian D.
- Date Published:
- Journal Name:
- PLOS ONE
- Volume:
- 16
- Issue:
- 5
- ISSN:
- 1932-6203
- Page Range / eLocation ID:
- e0251653
- Format(s):
- Medium: X
- Sponsoring Org:
- National Science Foundation
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- Free Publicly Accessible Full Text
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Accepted Manuscript1.0
- Journal Article:
- https://doi.org/10.1371/journal.pone.0251653
