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1. Bacterial response to the 2021 Orange County, California, oil spill was episodic but subtle relative to natural fluctuations

   https://doi.org/10.1128/spectrum.02267-24  

   Brock, Melissa L; Tavares-Reager, Joana F; Dong, Jialin; Larkin, Alyse A; Lam, Toan; Pineda, Nataly; Olivares, Christopher I; Mackey, Katherine_R M; Martiny, Adam C (May 2025, Microbiology Spectrum)

   Steven, Blaire (Ed.)

   ABSTRACT An oil spill began in October 2021 off the coast of Orange County, California, releasing 24,696 gallons of crude oil into coastal environments. Although oil spills, such as this one, are recurrent accidents along the California coast, no prior studies have been performed to examine the severity of the local bacterial response. A coastal 10-year time series of short-read metagenomes located within the impacted area allowed us to quantify the magnitude and duration of the disturbance relative to natural fluctuations. We found that the largest change in bacterial beta-diversity occurred at the end of October. The change in taxonomic beta-diversity corresponded with an increase in the sulfur-oxidizing cladeCandidatusThioglobus, an increase in the total relative abundance of potential hydrocarbon-degrading bacteria, and an anomalous decline in the picocyanobacteriaSynechococcus. Similarly, changes in function were related to anomalous declines in photosynthetic pathways and anomalous increases in sulfur metabolism pathways as well as aromatic degradation pathways. There was a lagged response in taxonomy and function to peaks in total PAHs. One week after peaks in total PAH concentrations, the largest shifts in taxonomy were observed, and 1 week after the taxonomy shifts were observed, unique functional changes were seen. This response pattern was observed twice during our sampling period, corresponding with the combined effect of resuspended PAHs and increased nutrient concentrations due to physical transport events. Thus, the impact of the spill on bacterial communities was temporally extended and demonstrates the need for continued monitoring for longer than 3 months after initial oil exposure.IMPORTANCEOil spills are common occurrences in waterways, releasing contaminants into the aquatic environment that persist for long periods of time. Bacterial communities are rapid responders to environmental disturbances, such as oil spills. Within bacterial communities, some members will be susceptible to the disturbance caused by crude oil components and will decline in abundance, whereas others will be opportunistic and will be able to use crude oil components for their metabolism. In many cases, when an oil spill occurs, it is difficult to assess the oil spill’s impact because no samples were collected prior to the accident. Here, we examined the bacterial response to the 2021 Orange County oil spill using a 10-year time series that lies within the impacted area. The results presented here are significant because (i) susceptible and opportunistic taxa to oil spills within the coastal California environment are identified and (ii) the magnitude and duration of thein situbacterial response is quantified for the first time. 

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2. Metagenomics and Quantitative Stable Isotope Probing Offer Insights into Metabolism of Polycyclic Aromatic Hydrocarbon Degraders in Chronically Polluted Seawater

   https://doi.org/10.1128/mSystems.00245-21  

   Sieradzki, Ella T.; Morando, Michael; Fuhrman, Jed A. (June 2021, mSystems)

   Rappe, Michael S. (Ed.)

   ABSTRACT Bacterial biodegradation is a significant contributor to remineralization of polycyclic aromatic hydrocarbons (PAHs)—toxic and recalcitrant components of crude oil as well as by-products of partial combustion chronically introduced into seawater via atmospheric deposition. The Deepwater Horizon oil spill demonstrated the speed at which a seed PAH-degrading community maintained by chronic inputs responds to acute pollution. We investigated the diversity and functional potential of a similar seed community in the chronically polluted Port of Los Angeles (POLA), using stable isotope probing with naphthalene, deep-sequenced metagenomes, and carbon incorporation rate measurements at the port and in two sites in the San Pedro Channel. We demonstrate the ability of the community of degraders at the POLA to incorporate carbon from naphthalene, leading to a quick shift in microbial community composition to be dominated by the normally rare Colwellia and Cycloclasticus . We show that metagenome-assembled genomes (MAGs) belonged to these naphthalene degraders by matching their 16S-rRNA gene with experimental stable isotope probing data. Surprisingly, we did not find a full PAH degradation pathway in those genomes, even when combining genes from the entire microbial community, leading us to hypothesize that promiscuous dehydrogenases replace canonical naphthalene degradation enzymes in this site. We compared metabolic pathways identified in 29 genomes whose abundance increased in the presence of naphthalene to generate genomic-based recommendations for future optimization of PAH bioremediation at the POLA, e.g., ammonium as opposed to urea, heme or hemoproteins as an iron source, and polar amino acids. IMPORTANCE Oil spills in the marine environment have a devastating effect on marine life and biogeochemical cycles through bioaccumulation of toxic hydrocarbons and oxygen depletion by hydrocarbon-degrading bacteria. Oil-degrading bacteria occur naturally in the ocean, especially where they are supported by chronic inputs of oil or other organic carbon sources, and have a significant role in degradation of oil spills. Polycyclic aromatic hydrocarbons are the most persistent and toxic component of crude oil. Therefore, the bacteria that can break those molecules down are of particular importance. We identified such bacteria at the Port of Los Angeles (POLA), one of the busiest ports worldwide, and characterized their metabolic capabilities. We propose chemical targets based on those analyses to stimulate the activity of these bacteria in case of an oil spill in the Port POLA. 

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3. Persistent and substantial impacts of the Deepwater Horizon oil spill on deep-sea megafauna

   https://doi.org/10.1098/rsos.191164  

   McClain, Craig R.; Nunnally, Clifton; Benfield, Mark C. (August 2019, Royal Society Open Science)

   The Deepwater Horizon spill is one of the largest environmental disasters with extensive impacts on the economic and ecological health of the Gulf of Mexico. Surface oil and coastal impacts received considerable attention, but the far larger oil spill in the deep ocean and its effects received considerably less examination. Based on 2017 ROV surveys within 500 m of the wellhead, we provide evidence of continued impacts on diversity, abundance and health of deep-sea megafauna. At locations proximal to the wellhead, megafaunal communities are more homogeneous than in unimpacted areas, lacking many taxonomic groups, and driven by high densities of arthropods. Degraded hydrocarbons at the site may be attracting arthropods. The scope of impacts may extend beyond the impacted sites with the potential for impacts to pelagic food webs and commercially important species. Overall, deep-sea ecosystem health, 7 years post spill, is recovering slowly and lingering effects may be extreme. 

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4. Estimates of Occupational Inhalation Exposures to Six Oil-Related Compounds on the Four Rig Vessels Responding to the Deepwater Horizon Oil Spill

   https://doi.org/10.1093/annweh/wxaa072  

   Huynh, Tran B; Groth, Caroline P; Ramachandran, Gurumurthy; Banerjee, Sudipto; Stenzel, Mark; Quick, Harrison; Blair, Aaron; Engel, Lawrence S; Kwok, Richard K; Sandler, Dale P; et al (October 2020, Annals of Work Exposures and Health)

   null (Ed.)

   Abstract Background The 2010 Deepwater Horizon (DWH) oil spill involved thousands of workers and volunteers to mitigate the oil release and clean-up after the spill. Health concerns for these participants led to the initiation of a prospective epidemiological study (GuLF STUDY) to investigate potential adverse health outcomes associated with the oil spill response and clean-up (OSRC). Characterizing the chemical exposures of the OSRC workers was an essential component of the study. Workers on the four oil rig vessels mitigating the spill and located within a 1852 m (1 nautical mile) radius of the damaged wellhead [the Discoverer Enterprise (Enterprise), the Development Driller II (DDII), the Development Driller III (DDIII), and the Helix Q4000] had some of the greatest potential for chemical exposures. Objectives The aim of this paper is to characterize potential personal chemical exposures via the inhalation route for workers on those four rig vessels. Specifically, we presented our methodology and descriptive statistics of exposure estimates for total hydrocarbons (THCs), benzene, toluene, ethylbenzene, xylene, and n-hexane (BTEX-H) for various job groups to develop exposure groups for the GuLF STUDY cohort. Methods Using descriptive information associated with the measurements taken on various jobs on these rig vessels and with job titles from study participant responses to the study questionnaire, job groups [unique job/rig/time period (TP) combinations] were developed to describe groups of workers with the same or closely related job titles. A total of 500 job groups were considered for estimation using the available 8139 personal measurements. We used a univariate Bayesian model to analyze the THC measurements and a bivariate Bayesian regression framework to jointly model the measurements of THC and each of the BTEX-H chemicals separately, both models taking into account the many measurements that were below the analytic limit of detection. Results Highest THC exposures occurred in TP1a and TP1b, which was before the well was mechanically capped. The posterior medians of the arithmetic mean (AM) ranged from 0.11 ppm (‘Inside/Other’, TP1b, DDII; and ‘Driller’, TP3, DDII) to 14.67 ppm (‘Methanol Operations’, TP1b, Enterprise). There were statistical differences between the THC AMs by broad job groups, rigs, and time periods. The AMs for BTEX-H were generally about two to three orders of magnitude lower than the THC AMs, with benzene and ethylbenzene measurements being highly censored. Conclusions Our results add new insights to the limited literature on exposures associated with oil spill responses and support the current epidemiologic investigation of potential adverse health effects of the oil spill. 

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5. Lagrangian coherence and source of water of Loop Current Frontal Eddies in the Gulf of Mexico. Progress in Oceanography.

   Hiron, L. (August 2022, Progress in Oceanography)

   Elsevier Publishing Company (Ed.)

   Loop Current Frontal Eddies (LCFEs) are known to intensify and assist in the Loop Current (LC) eddy shedding. In addition to interacting with the LC, these eddies also modify the circulation in the eastern Gulf of Mexico by attracting water and passive tracers such as chlorophyll, Mississippi freshwater, and pollutants to the LC-LCFE front. During the 2010 Deepwater Horizon oil spill, part of the oil was entrained not only in the LC-LCFE front but also inside an LCFE, where it remained for weeks. This study assesses the ability of the LCFEs to transport water and passive tracers without exchange with the exterior (i.e., Lagrangian coherence) using altimetry and a high-resolution model. The following open questions are answered: (1) How long can the LCFEs remain Lagrangian coherent at and below the surface? (2) What is the source of water for the formation of LCFEs? (3) Can the formation of Lagrangian coherent LCFEs attract shelf water? Strong frontal eddies leading to LC eddy shedding are investigated using a 1-km resolution model for the Gulf of Mexico and altimetry. The results show that LCFEs are composed of waters originating from the outer band of the LC front, the region north of the LC, and the western West Florida Shelf and Mississippi/Alabama/Florida shelf, and potentially drive cross-shelf exchange of particles, water properties, and nutrients. At depth (≈180 m), most LCFE water comes from the outer band of the LC front in the form of smaller frontal eddies. Once formed, LCFEs can transport water and passive tracers in their interior without exchange with the exterior for weeks: these eddies remained Lagrangian coherent for up to 25 days in the altimetry dataset and 18 days at the surface and 29 days at depth (≈180 m) in the simulation. LCFE can remain Lagrangian coherent up to a depth of ≈ 560 m. Additional analyses show that the LCFE involved in the Deepwater Horizon oil spill formed from water near the oil rig location, in agreement with previous studies. Temperature-salinity diagrams from a high-resolution model and aircraft expendable profilers show that LCFEs are composed of Gulf of Mexico water as opposed to LC water. Therefore, LCFE formation and propagation actively modify the surrounding circulation and affect the evolution of the flow and the transport of oil and other passive tracers in the Eastern Gulf of Mexico. 

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