Abstract Three-dimensional bioprinting is a rapidly growing field attempting to recreate functional tissues for medical and pharmaceutical purposes. Development of functional tissue requires deposition of multiple biomaterials encapsulating multiple cell types i.e. bio-ink necessitating switching ability between bio-inks. Existing systems use more than one print head to achieve this complex interchangeable deposition, which decreases efficiency, structural integrity, and accuracy. In this research, we developed a nozzle system capable of switching between multiple bio-inks with continuous deposition ensuring the minimum transition distance so that precise deposition transitioning can be achieved. Finally, the effect of rheological properties of different bio-material compositions on the transition distance is investigated by fabricating the sample scaffolds.
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Designing an Interchangeable Multi-Material Nozzle System for 3D Bioprinting Process
Three-dimensional bioprinting is a rapidly growing field attempting to recreate functional tissues for medical and pharmaceutical purposes. Development of functional tissue requires deposition of multiple biomaterials encapsulating multiple cell types i.e. bio-ink necessitating switching ability between bio-inks. Existing systems use more than one print head to achieve this complex interchangeable deposition, which decreases efficiency, structural integrity, and accuracy. In this research, we developed a nozzle system capable of switching between multiple bio-inks with continuous deposition ensuring the minimum transition distance so that precise deposition transitioning can be achieved. Finally, the effect of rheological properties of different bio-material compositions on the transition distance is investigated by fabricating the sample scaffolds.
more » « less- Award ID(s):
- 1757371
- NSF-PAR ID:
- 10294606
- Date Published:
- Journal Name:
- Proceedings of the ASME 2021 16th International Manufacturing Science and Engineering Conference
- Format(s):
- Medium: X
- Sponsoring Org:
- National Science Foundation
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Abstract Three-dimensional (3D) bio-printing is a rapidly growing field attempting to recreate functional tissues for medical and pharmaceutical purposes. The printability of multiple materials encapsulating various living cells can take this emerging effort closer to tissue regeneration. In our earlier research, we designed a Y-like nozzle connector system capable of switching materials between more than one filament with continuous deposition. The device had a fixed switching angle, was made from plastic, and was suitable for one-time use. This paper presents the extension of our previously proposed nozzle system. We considered 30°, 45°, 60°, and 90° angles (vertical and tilted) between the two materials and chose stainless steel as a material to fabricate those nozzle connectors. The overall material switching time was recorded and compared to analyze the effects of those various angles. Our previously developed hybrid hydrogel (4% Alginate and 4% Carboxymethyl Cellulose, CMC) was used as a test material to flow through the nozzle system. These in-house fabricated nozzle connectors are reusable, easy to clean, and sterile, allowing smooth material transition and flow.
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Eds: Babski-Reeves, K ; Eksioglu, B ; Hampton, D. (Ed.)The emerging field of three-dimensional bio-printing seeks to recreate functional tissues for medical and pharmaceutical purposes. With the ability to print diverse materials containing different living cells, this growing area may bring us closer to achieving tissue regeneration. In previous research, we developed a Y-shaped nozzle connection device that facilitated the continuous deposition of materials across multiple filaments. This plastic device had a fixed switching angle and was intended for single use. In this study, we present an extension of our previous nozzle system. To fabricate the nozzle connectors, we chose stainless steel and considered angles of 300, 450, and 900 (both vertical and tilted) between the two materials. The total material switching time was recorded and compared to analyze the effects of these angles. We used our previously developed hybrid hydrogel (4% Alginate and 4% Carboxymethyl Cellulose, CMC) as a test material to flow through the nozzle system. These in-house fabricated nozzle connectors are reusable, and sterile and enable smooth material transition and flow.more » « less
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Abstract 3D bioprinting additively assembles bio‐inks to manufacture tissue‐mimicking biological constructs, but with the typical building blocks limited to 1D filaments. Here, it is developed a voxelated bioprinting technique for the digital assembly of spherical particles (DASP), which are effectively 0D voxels—the basic unit of 3D structures. It is shown that DASP enables on‐demand generation, deposition, and assembly of viscoelastic bio‐ink droplets. A two‐parameter diagram is developed to outline the viscoelasticity of bio‐inks required for printing spherical particles of good fidelity. Moreover, a strategy is developed for engineering bio‐inks with independently controllable viscoelasticity and mesh size, two of the most important yet intrinsically coupled physical properties of biomaterials. Using DASP, mechanically robust, multiscale porous scaffolds composed of interconnected yet distinguishable hydrogel particles are created. Finally, it is demonstrated the application of the scaffolds in encapsulating human pancreatic islets for sustained responsive insulin release. Together with the knowledge of bio‐ink design, DASP might be used to engineer highly heterogeneous, yet tightly organized tissue constructs for therapeutic applications.
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Abstract Direct ink writing is a facile method that enables biological, structural, and functional materials to be printed in three dimensions (3D). To date, this extrusion‐based method has primarily been used to soft materials in a layer‐wise manner on planar substrates. However, many emerging applications would benefit from the ability to conformally print materials of varying composition on substrates with arbitrary topography. Here, a high throughput platform based on multimaterial multinozzle adaptive 3D printing (MMA‐3DP) that provides independent control of nozzle height and seamless switching between inks is reported. To demonstrate the MMA‐3DP platform, conformally pattern viscoelastic inks composed of triblock copolymer, gelatin, and photopolymerizable polyacrylate materials onto complex substrates of varying topography, including those with surface defects that mimic skin abrasions or deep gouges. This platform opens new avenues for rapidly patterning soft materials for structural, functional, and biomedical applications.
- Free Publicly Accessible Full Text
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Accepted Manuscript1.0
- Conference Paper:
- https://doi.org/10.1115/msec2021-63471
