skip to main content
US FlagAn official website of the United States government
dot gov icon
Official websites use .gov
A .gov website belongs to an official government organization in the United States.
https lock icon
Secure .gov websites use HTTPS
A lock ( lock ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.


Title: ADMM approach for efficient iterative tomographic deconvolution reconstruction of 3D quantitative phase images
Tomographic deconvolution phase microscopy (TDPM) is a promising approach for 3D quantitative imaging of phase objects such as biological cells and optical fibers. In the present work, the alternating direction method of multipliers (ADMM) is applied to TDPM to shorten its image acquisition and processing times while simultaneously improving its accuracy. ADMM-TDPM is used to optimize the image fidelity by minimizing Gaussian noise and by using total variation regularization with the constraints of nonnegativity and known zeros. ADMM-TDPM can reconstruct phase objects that are shift variant in three spatial dimensions. ADMM-TDPM achieves speedups of 5x in image acquisition time and greater than 10x in image processing time with accompanying higher accuracy compared to TDPM.  more » « less
Award ID(s):
1915971
PAR ID:
10304744
Author(s) / Creator(s):
; ;
Publisher / Repository:
Optical Society of America
Date Published:
Journal Name:
Applied Optics
Volume:
60
Issue:
27
ISSN:
1559-128X; APOPAI
Format(s):
Medium: X Size: Article No. 8485
Size(s):
Article No. 8485
Sponsoring Org:
National Science Foundation
More Like this
  1. Abstract Conventional imaging and recognition systems require an extensive amount of data storage, pre-processing, and chip-to-chip communications as well as aberration-proof light focusing with multiple lenses for recognizing an object from massive optical inputs. This is because separate chips (i.e., flat image sensor array, memory device, and CPU) in conjunction with complicated optics should capture, store, and process massive image information independently. In contrast, human vision employs a highly efficient imaging and recognition process. Here, inspired by the human visual recognition system, we present a novel imaging device for efficient image acquisition and data pre-processing by conferring the neuromorphic data processing function on a curved image sensor array. The curved neuromorphic image sensor array is based on a heterostructure of MoS2and poly(1,3,5-trimethyl-1,3,5-trivinyl cyclotrisiloxane). The curved neuromorphic image sensor array features photon-triggered synaptic plasticity owing to its quasi-linear time-dependent photocurrent generation and prolonged photocurrent decay, originated from charge trapping in the MoS2-organic vertical stack. The curved neuromorphic image sensor array integrated with a plano-convex lens derives a pre-processed image from a set of noisy optical inputs without redundant data storage, processing, and communications as well as without complex optics. The proposed imaging device can substantially improve efficiency of the image acquisition and recognition process, a step forward to the next generation machine vision. 
    more » « less
  2. Quantum ghost imaging (QGI) leverages correlations between entangled photon pairs to reconstruct an image using light that has never physically interacted with an object. Despite extensive research interest, this technique has long been hindered by slow acquisition speeds, due to the use of raster-scanned detectors or the slow response of intensified cameras. Here, we utilize a single-photon-sensitive time-stamping camera to perform QGI at ultra-low-light levels with rapid data acquisition and processing times, achieving high-resolution and high-contrast images in under 1 min. Our work addresses the trade-off between image quality, optical power, data acquisition time, and data processing time in QGI, paving the way for practical applications in biomedical and quantum-secured imaging. 
    more » « less
  3. We propose a simple, cost-effective method for synchronized phase contrast and fluorescence video acquisition in live samples. Counter-phased pulses of phase contrast illumination and fluorescence excitation light are synchronized with the exposure of the two fields of an interlaced camera sensor. This results in a video sequence in which each frame contains both exposure modes, each in half of its pixels. The method allows real-time acquisition and display of synchronized and spatially aligned phase contrast and fluorescence image sequences that can be separated by de-interlacing in two independent videos. The method can be implemented on any fluorescence microscope with a camera port without needing to modify the optical path. 
    more » « less
  4. The task of automatically assessing and adjusting image quality, when capturing face images using any band-specific camera sensor, can be achieved by eliminating a variety of acquisition parameters such as illumination. One such parameter related to image quality is sharpness. If it is not accurately estimated during data collection, it may affect the quality of the overall face image dataset and thus face recognition accuracy. While manually focusing each camera on the target (human face) can result in sharp looking face images, the process can be cumbersome for the operators and the subjects and, thus, it increases data collection acquisition time. In this work, we developed an electromechanical based system that automatically assesses face image sharpness, prior to capture rather than necessitating post-processing schemes. Various blur quality factors and constraints have been empirically evaluated, before determining the algorithmic steps of our proposed system. This paper discusses the implementation of this system in a live operating system. 
    more » « less
  5. The applications of conventional ptychography are limited by its relatively low resolution and throughput in the visible light regime. The new development of coded ptychography (CP) has addressed these issues and achieved the highest numerical aperture for large-area optical imaging in a lensless configuration. A high-quality reconstruction of CP relies on precise tracking of the coded sensor’s positional shifts. The coded layer on the sensor, however, prevents the use of cross correlation analysis for motion tracking. Here we derive and analyze the motion tracking model of CP. A novel, to the best of our knowledge, remote referencing scheme and its subsequent refinement pipeline are developed for blind image acquisition. By using this approach, we can suppress the correlation peak caused by the coded surface and recover the positional shifts with deep sub-pixel accuracy. In contrast with common positional refinement methods, the reported approach can be disentangled from the iterative phase retrieval process and is computationally efficient. It allows blind image acquisition without motion feedback from the scanning process. It also provides a robust and reliable solution for implementing ptychography with high imaging throughput. We validate this approach by performing high-resolution whole slide imaging of bio-specimens. 
    more » « less