skip to main content


Title: Aquatic Eddy Covariance: The Method and Its Contributions to Defining Oxygen and Carbon Fluxes in Marine Environments
Aquatic eddy covariance (AEC) is increasingly being used to study benthic oxygen (O 2 ) flux dynamics, organic carbon cycling, and ecosystem health in marine and freshwater environments. Because it is a noninvasive technique, has a high temporal resolution (∼15 min), and integrates over a large area of the seafloor (typically 10–100 m 2 ), it has provided new insights on the functioning of aquatic ecosystems under naturally varying in situ conditions and has given us more accurate assessments of their metabolism. In this review, we summarize biogeochemical, ecological, and biological insightsgained from AEC studies of marine ecosystems. A general finding for all substrates is that benthic O 2 exchange is far more dynamic than earlier recognized, and thus accurate mean values can only be obtained from measurements that integrate over all timescales that affect the local O 2 exchange. Finally, we highlight new developments of the technique, including measurements of air–water gas exchange and long-term deployments.  more » « less
Award ID(s):
1851424 1832221 2049177 1824144
NSF-PAR ID:
10321509
Author(s) / Creator(s):
; ; ; ;
Date Published:
Journal Name:
Annual Review of Marine Science
Volume:
14
Issue:
1
ISSN:
1941-1405
Format(s):
Medium: X
Sponsoring Org:
National Science Foundation
More Like this
  1. Abstract

    The aquatic eddy covariance technique is increasingly used to determine oxygen (O2) fluxes over benthic ecosystems. The technique uses O2measuring systems that have a high temporal and numerical resolution. In this study, we performed a series of lab and field tests to assess a new optical submersible O2meter designed for aquatic eddy covariance measurements and equipped with an existing ultra‐high speed optical fiber sensor. The meter has a 16‐bit digital‐to‐analog‐signal conversion that produces a 0–5 V output at a rate up to 40 Hz. The device was paired with an acoustic Doppler velocimeter. The combined meter and fiber‐optic O2sensor's response time was significantly faster in O2‐undersaturated water compared to in O2‐supersaturated water (0.087 vs. 0.12 s), but still sufficiently fast for aquatic eddy covariance measurements. The O2optode signal was not sensitive to variations in water flow or light exposure. However, the response time was affected by the direction of the flow. When the sensor tip was exposed to a flow from the back rather than the front, the response time increased by 37%. The meter's internal signal processing time was determined to be ~ 0.05 s, a delay that can be corrected for during postprocessing. In order for the built‐in temperature correction to be accurate, the meter should always be submerged with the fiber‐optic sensor. In multiple 21–47 h field tests, the system recorded consistently high‐quality, low‐noise O2flux data. Overall, the new meter is a powerful option for collecting robust aquatic eddy covariance data.

     
    more » « less
  2. Organic carbon mineralization and nutrient cycling in benthic environments are critically important for their biogeochemical functioning, but are poorly understood in coastal upwelling systems. The main objective of this study was to determine benthic oxygen fluxes in a muddy sediment in the Ria de Vigo (NW Iberian coastal upwelling), by applying the aquatic eddy covariance (AEC) technique during 2 campaigns in different seasons (June and October 2017). The main drivers of benthic fluxes were studied and compared among days in each season and between seasons. The 2 campaigns were characterized by an upwelling-relaxation period followed by a downwelling event, the last of which was due to the extratropical cyclone Ophelia in October. The mean (±SD) seasonal benthic oxygen fluxes were not significantly different for the 2 campaigns despite differences in hydrodynamic and biogeochemical conditions (June: -20.9 ± 7.1 mmol m -2 d -1 vs. October: -26.5 ± 3.1 mmol m -2 d -1 ). Benthic fluxes were controlled by different drivers depending on the season. June was characterized by sinking labile organic material, which enhanced benthic fluxes in the downwelling event, whereas October had a significantly higher bottom velocity that stimulated the benthic fluxes. Finally, a comparison with a large benthic chamber (0.50 m 2 ) was made during October. Despite methodological differences between AEC and chamber measurements, concurrent fluxes agreed within an acceptable margin (AEC:benthic chamber ratio = 0.78 ± 0.13; mean ± SD). Bottle incubations of water sampled from the chamber interior indicated that mineralization could explain this difference. These results show the importance of using non-invasive techniques such as AEC to resolve benthic flux dynamics. 
    more » « less
  3. Abstract

    Seagrass meadows play an important role in “blue carbon” sequestration and storage, but their dynamic metabolism is not fully understood. In a denseZostera marinameadow, we measured benthic O2fluxes by aquatic eddy covariance, water column concentrations of O2, and partial pressures of CO2(pCO2) over 21 full days during peak growing season in April and June. Seagrass metabolism, derived from the O2flux, varied markedly between the 2 months as biomass accumulated and water temperature increased from 16°C to 28°C, triggering a twofold increase in respiration and a trophic shift of the seagrass meadow from being a carbon sink to a carbon source. Seagrass metabolism was the major driver of diurnal fluctuations in water column O2concentration and pCO2, ranging from 173 to 377 μmol L−1and 193 to 859 ppmv, respectively. This 4.5‐fold variation in pCO2was observed despite buffering by the carbonate system. Hysteresis in diurnal water column pCO2vs. O2concentration was attributed to storage of O2and CO2in seagrass tissue, air–water exchange of O2and CO2, and CO2storage in surface sediment. There was a ~ 1:1 mol‐to‐mol stoichiometric relationship between diurnal fluctuations in concentrations of O2and dissolved inorganic carbon. Our measurements showed no stimulation of photosynthesis at high CO2and low O2concentrations, even though CO2reached levels used in IPCC ocean acidification scenarios. This field study does not support the notion that seagrass meadows may be “winners” in future oceans with elevated CO2concentrations and more frequent temperature extremes.

     
    more » « less
  4. Abstract. The aquatic eddy covariance technique stands out as a powerful method for benthic O2 flux measurements in shelf environments because itintegrates effects of naturally varying drivers of the flux such as current flow and light. In conventional eddy covariance instruments, the timeshift caused by spatial separation of the measuring locations of flow and O2 concentration can produce substantial flux errors that aredifficult to correct. We here introduce a triple O2 sensor eddy covariance instrument (3OEC) that by instrument design eliminates theseerrors. This is achieved by positioning three O2 sensors around the flow measuring volume, which allows the O2concentration to be calculated at the point of the current flow measurements. The new instrument was tested in an energetic coastal environment with highly permeablecoral reef sands colonised by microphytobenthos. Parallel deployments of the 3OEC and a conventional eddy covariance system (2OEC) demonstrate thatthe new instrument produces more consistent fluxes with lower error margin. 3OEC fluxes in general were lower than 2OEC fluxes, and the nighttimefluxes recorded by the two instruments were statistically different. We attribute this to the elimination of uncertainties associated with the timeshift correction. The deployments at ∼ 10 m water depth revealed high day- and nighttime O2 fluxes despite the relatively loworganic content of the coarse sediment and overlying water. High light utilisation efficiency of the microphytobenthos and bottom currents increasingpore water exchange facilitated the high benthic production and coupled respiration. 3OEC measurements after sunset documented a gradual transfer ofnegative flux signals from the small turbulence generated at the sediment–water interface to the larger wave-dominated eddies of the overlying watercolumn that still carried a positive flux signal, suggesting concurrent fluxes in opposite directions depending on eddy size and a memory effect oflarge eddies. The results demonstrate that the 3OEC can improve the precision of benthic flux measurements, including measurements in environmentsconsidered challenging for the eddy covariance technique, and thereby produce novel insights into the mechanisms that control flux. We consider thefluxes produced by this instrument for the permeable reef sands the most realistic achievable with present-day technology. 
    more » « less
  5. Scientific Significance Statement

    Metabolic stoichiometry predicts that dissolved oxygen (O2) and carbon dioxide (CO2) in aquatic ecosystems should covary inversely; however, field observations often diverge from theoretical expectations. Here, we propose a suite of metrics describing this O2and CO2decoupling and introduce a conceptual framework for interpreting these metrics within aquatic ecosystems. Within this framework, we interpret cross‐system patterns of high‐frequency O2and CO2measurements in 11 northern lakes and extract emergent insights into the metabolic behavior and the simultaneous roles of chemical and physical forcing in shaping ecosystem processes. This approach leverages the power of high‐frequency paired O2–CO2measurements, and yields a novel, integrative aquatic system typology which can also be applicable more broadly to streams and rivers, wetlands and marine systems.

     
    more » « less