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Spectroscopic single-molecule localization microscopy (sSMLM) simultaneously provides spatial localization and spectral information of individual single-molecules emission, offering multicolor super-resolution imaging of multiple molecules in a single sample with the nanoscopic resolution. However, this technique is limited by the requirements of acquiring a large number of frames to reconstruct a super-resolution image. In addition, multicolor sSMLM imaging suffers from spectral cross-talk while using multiple dyes with relatively broad spectral bands that produce cross-color contamination. Here, we present a computational strategy to accelerate multicolor sSMLM imaging. Our method uses deep convolution neural networks to reconstruct high-density multicolor super-resolution images from low-density, contaminated multicolor images rendered using sSMLM datasets with much fewer frames, without compromising spatial resolution. High-quality, super-resolution images are reconstructed using up to 8-fold fewer frames than usually needed. Thus, our technique generates multicolor super-resolution images within a much shorter time, without any changes in the existing sSMLM hardware system. Two-color and three-color sSMLM experimental results demonstrate superior reconstructions of tubulin/mitochondria, peroxisome/mitochondria, and tubulin/mitochondria/peroxisome in fixed COS-7 and U2-OS cells with a significant reduction in acquisition time.
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STSRNet: Deep Joint Space-Time Super-Resolution for Vector Field Visualization
We propose STSRNet, a joint space-time super-resolution deep learning based model for time-varying vector field data. Our method is designed to reconstruct high temporal resolution (HTR) and high spatial resolution (HSR) vector fields sequence from the corresponding low-resolution key frames. For large scale simulations, only data from a subset of time steps with reduced spatial resolution can be stored for post-hoc analysis. In this paper, we leverage a deep learning model to capture the non-linear complex changes of vector field data with a two-stage architecture: the first stage deforms a pair of low spatial resolution (LSR) key frames forward and backward to generate the intermediate LSR frames, and the second stage performs spatial super-resolution to output the high-resolution sequence. Our method is scalable and can handle different data sets. We demonstrate the effectiveness of our framework with several data sets through quantitative and qualitative evaluations.
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- Award ID(s):
- 1955764
- PAR ID:
- 10328006
- Date Published:
- Journal Name:
- IEEE computer graphics and applications
- Volume:
- 41
- ISSN:
- 0272-1716
- Format(s):
- Medium: X
- Sponsoring Org:
- National Science Foundation
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