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Title: Tilapia prolactin cells are thermosensitive osmoreceptors
Prolactin (PRL) cells within the rostral pars distalis (RPD) of euryhaline and eurythermal Mozambique tilapia, Oreochromis mossambicus, rapidly respond to a hyposmotic stimulus by releasing two distinct PRL isoforms, PRL 188 and PRL 177 . Here, we describe how environmentally relevant temperature changes affected mRNA levels of prl 188 and prl 177 and the release of immunoreactive prolactins from RPDs and dispersed PRL cells. When applied under isosmotic conditions (330 mosmol/kgH 2 O), a 6°C rise in temperature stimulated the release of PRL 188 and PRL 177 from both RPDs and dispersed PRL cells under perifusion. When exposed to this same change in temperature, ∼50% of dispersed PRL cells gradually increased in volume by ∼8%, a response partially inhibited by the water channel blocker, mercuric chloride. Following their response to increased temperature, PRL cells remained responsive to a hyposmotic stimulus (280 mosmol/kgH 2 O). The mRNA expression of transient potential vanilloid 4, a Ca 2+ -channel involved in hyposmotically induced PRL release, was elevated in response to a rise in temperature in dispersed PRL cells and RPDs at 6 and 24 h, respectively; prl 188 and prl 177 mRNAs were unaffected. Our findings indicate that thermosensitive PRL release is mediated, at least partially, through a cell-volume-dependent pathway similar to how osmoreceptive PRL release is achieved.  more » « less
Award ID(s):
1755016 1755131
PAR ID:
10333603
Author(s) / Creator(s):
; ; ; ; ;
Date Published:
Journal Name:
American Journal of Physiology-Regulatory, Integrative and Comparative Physiology
Volume:
322
Issue:
6
ISSN:
0363-6119
Page Range / eLocation ID:
R609 to R619
Format(s):
Medium: X
Sponsoring Org:
National Science Foundation
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