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Title: Critical Assessment of Metagenome Interpretation: the second round of challenges
Abstract Evaluating metagenomic software is key for optimizing metagenome interpretation and focus of the Initiative for the Critical Assessment of Metagenome Interpretation (CAMI). The CAMI II challenge engaged the community to assess methods on realistic and complex datasets with long- and short-read sequences, created computationally from around 1,700 new and known genomes, as well as 600 new plasmids and viruses. Here we analyze 5,002 results by 76 program versions. Substantial improvements were seen in assembly, some due to long-read data. Related strains still were challenging for assembly and genome recovery through binning, as was assembly quality for the latter. Profilers markedly matured, with taxon profilers and binners excelling at higher bacterial ranks, but underperforming for viruses and Archaea. Clinical pathogen detection results revealed a need to improve reproducibility. Runtime and memory usage analyses identified efficient programs, including top performers with other metrics. The results identify challenges and guide researchers in selecting methods for analyses.  more » « less
Award ID(s):
1845890 1919691 1936791 2107108
NSF-PAR ID:
10334129
Author(s) / Creator(s):
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Date Published:
Journal Name:
Nature Methods
Volume:
19
Issue:
4
ISSN:
1548-7091
Page Range / eLocation ID:
429 to 440
Format(s):
Medium: X
Sponsoring Org:
National Science Foundation
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