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1. Long‐term nitrogen deposition inhibits soil priming effects by enhancing phosphorus limitation in a subtropical forest

   https://doi.org/10.1111/gcb.16718  

   Wang, Xiaohong; Li, Shiyining; Zhu, Biao; Homyak, Peter M.; Chen, Guangshui; Yao, Xiaodong; Wu, Dongmei; Yang, Zhijie; Lyu, Maokui; Yang, Yusheng (April 2023, Global Change Biology)

   Abstract It is widely accepted that phosphorus (P) limits microbial metabolic processes and thus soil organic carbon (SOC) decomposition in tropical forests. Global change factors like elevated atmospheric nitrogen (N) deposition can enhance P limitation, raising concerns about the fate of SOC. However, how elevated N deposition affects the soil priming effect (PE) (i.e., fresh C inputs induced changes in SOC decomposition) in tropical forests remains unclear. We incubated soils exposed to 9 years of experimental N deposition in a subtropical evergreen broadleaved forest with two types of¹³C‐labeled substrates of contrasting bioavailability (glucose and cellulose) with and without P amendments. We found that N deposition decreased soil total P and microbial biomass P, suggesting enhanced P limitation. In P unamended soils, N deposition significantly inhibited the PE. In contrast, adding P significantly increased the PE under N deposition and by a larger extent for the PE of cellulose (PE_cellu) than the PE of glucose (PE_glu). Relative to adding glucose or cellulose solely, adding P with glucose alleviated the suppression of soil microbial biomass and C‐acquiring enzymes induced by N deposition, whereas adding P with cellulose attenuated the stimulation of acid phosphatase (AP) induced by N deposition. Across treatments, the PE_gluincreased as C‐acquiring enzyme activity increased, whereas the PE_celluincreased as AP activity decreased. This suggests that P limitation, enhanced by N deposition, inhibits the soil PE through varying mechanisms depending on substrate bioavailability; that is, P limitation regulates the PE_gluby affecting soil microbial growth and investment in C acquisition, whereas regulates the PE_celluby affecting microbial investment in P acquisition. These findings provide new insights for tropical forests impacted by N loading, suggesting that expected changes in C quality and P limitation can affect the long‐term regulation of the soil PE. 

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2. Wall-to-wall optimal transport in two dimensions

   https://doi.org/10.1017/jfm.2020.42  

   Souza, Andre N.; Tobasco, Ian; Doering, Charles R. (April 2020, Journal of Fluid Mechanics)

   Gradient ascent methods are developed to compute incompressible flows that maximize heat transport between two isothermal no-slip parallel walls. Parameterizing the magnitude of the velocity fields by a Péclet number $Pe$ proportional to their root-mean-square rate of strain, the schemes are applied to compute two-dimensional flows optimizing convective enhancement of diffusive heat transfer, i.e. the Nusselt number $Nu$ up to $$Pe\approx 10^{5}$$ . The resulting transport exhibits a change of scaling from $$Nu-1\sim Pe^{2}$$ for $Pe<10$ in the linear regime to $$Nu\sim Pe^{0.54}$$ for $$Pe>10^{3}$$ . Optimal fields are observed to be approximately separable, i.e. products of functions of the wall-parallel and wall-normal coordinates. Analysis employing a separable ansatz yields a conditional upper bound $${\lesssim}Pe^{6/11}=Pe^{0.\overline{54}}$$ as $$Pe\rightarrow \infty$$ similar to the computationally achieved scaling. Implications for heat transfer in buoyancy-driven Rayleigh–Bénard convection are discussed. 

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3. Oxidative regulation of the mechanical strength of a C–S bond

   https://doi.org/10.1039/D0SC04381H  

   Lin, Yangju; Craig, Stephen L. (October 2020, Chemical Science)

   null (Ed.)

   The mechanical strength of individual polymer chains is believed to underlie a number of performance metrics in bulk materials, including adhesion and fracture toughness. Methods by which the intrinsic molecular strength of the constituents of a given polymeric material might be switched are therefore potentially useful both for applications in which triggered property changes are desirable, and as tests of molecular theories for bulk behaviors. Here we report that the sequential oxidation of sulfide containing polyesters ( PE-S ) to the corresponding sulfoxide ( PE-SO ) and then sulfone ( PE-SO2 ) first weakens (sulfoxide), and then enhances (sulfone), the effective mechanical integrity of the polymer backbone; PE-S ∼ PE-SO2 > PE-SO . The relative mechanical strength as a function of oxidation state is revealed through the use of gem -dichlorocyclopropane nonscissile mechanophores as an internal standard, and the observed order agrees well with the reported bond dissociation energies of C–S bonds in each species and with the results of CoGEF modeling. 

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4. Dynamic changes in primexine during the tetrad stage of pollen development

   https://doi.org/10.1093/plphys/kiab426  

   Wang, Rui; Owen, Heather A; Dobritsa, Anna A (September 2021, Plant Physiology)

   Abstract Formation of pollen wall exine is preceded by the development of several transient layers of extracellular materials deposited on the surface of developing pollen grains. One such layer is primexine (PE), a thin, ephemeral structure that is present only for a short period of time and is difficult to visualize and study. Recent genetic studies suggested that PE is a key factor in the formation of exine, making it critical to understand its composition and the dynamics of its formation. In this study, we used high-pressure frozen/freeze-substituted samples of developing Arabidopsis (Arabidopsis thaliana) pollen for a detailed transmission electron microscopy analysis of the PE ultrastructure throughout the tetrad stage of pollen development. We also analyzed anthers from wild-type Arabidopsis and three mutants defective in PE formation by immunofluorescence, carefully tracing several carbohydrate epitopes in PE and nearby anther tissues during the tetrad and the early free-microspore stages. Our analyses revealed likely sites where these carbohydrates are produced and showed that the distribution of these carbohydrates in PE changes significantly during the tetrad stage. We also identified tools for staging tetrads and demonstrate that components of PE undergo changes resembling phase separation. Our results indicate that PE behaves like a much more dynamic structure than has been previously appreciated and clearly show that Arabidopsis PE creates a scaffolding pattern for formation of reticulate exine. 

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5. Liquid Crystals as Multifunctional Interfaces for Trapping and Characterizing Colloidal Microplastics

   https://doi.org/10.1002/smll.202207802  

   Mukherjee, Fiona; Shi, Anye; Wang, Xin; You, Fengqi; Abbott, Nicholas L. (March 2023, Small)

   Abstract Identifying and removing microplastics (MPs) from the environment is a global challenge. This study explores how the colloidal fraction of MPs assemble into distinct 2D patterns at aqueous interfaces of liquid crystal (LC) films with the goal of developing surface‐sensitive methods for identifying MPs. Polyethylene (PE) and polystyrene (PS) microparticles are measured to exhibit distinct aggregation patterns, with addition of anionic surfactant amplifying differences in PS/PE aggregation patterns: PS changes from a linear chain‐like morphology to a singly dispersed state with increasing surfactant concentration whereas PE forms dense clusters at all surfactant concentrations. Statistical analysis of assembly patterns using deep learning image recognition models yields accurate classification, with feature importance analysis confirming that dense, multibranched assemblies are unique features of PE relative to PS. Microscopic characterization of LC ordering at the microparticle surfaces leads to predict LC‐mediated interactions (due to elastic strain) with a dipolar symmetry, a prediction consistent with the interfacial organization of PS but not PE. Further analysis leads to conclude that PE microparticles, due to their polycrystalline nature, possess rough surfaces that lead to weak LC elastic interactions and enhanced capillary forces. Overall, the results highlight the potential utility of LC interfaces for rapid identification of colloidal MPs based on their surface properties. 

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