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1. Variable extended depth of field imaging using freeform optics

   https://doi.org/10.1117/12.2568723  

   Moein, Sara; Suleski, Thomas J. (August 2020, SPIE Optical Engineering + Applications, 2020, Online Only)

   Hahlweg, Cornelius F.; Mulley, Joseph R. (Ed.)

   Increasing depth of field in imaging systems can be beneficial, particularly for systems with high numerical apertures and short depth of field, such as microscopy. Extending depth of field has been previously demonstrated, for example, using non-rotationally symmetric (freeform) components such as cubic and logarithmic phase plates. Such fixed phase plates are generally designed for a specific optical system, so a different phase plate is required for each system. Methods that enable variable extended depth of field for multiple optical systems could provide benefits by reducing the number of required components and costs. In this paper, we explore the design of a single pair of transmissive freeform surfaces to enable extended depth of field for multiple lenses with different numerical apertures through relative translation of the freeform components. This work builds on the concept of an Alvarez lens, in which one pair of transmissive XY-polynomial freeform surfaces generates variable optical power through lateral relative shifts between the surfaces. The presented approach is based on the design of multiple fixed phase plates to optimize the through-focus Modulation Transfer Function (MTF) for imaging lenses of given numerical apertures. The freeform surface equation for the desired variable phase plate pair is then derived and the relative shift amounts between the freeform surfaces are calculated to enable extended depth of field for multiple imaging lenses with different numerical aperture values. Design approaches and simulation results will be discussed. © (2020) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only. Citation Download Citation Sara Moein and Thomas J. Suleski "Variable extended depth of field imaging using freeform optics", Proc. SPIE 11483, Novel Optical Systems, Methods, and Applications XXIII, 114830G (21 August 2020); https://doi.org/10.1117/12.2568723 

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2. Design, fabrication, and characterization of a tunable LED-based illuminator using refractive freeform arrays

   https://doi.org/10.1364/OE.474842  

   Shadalou, Shohreh; Gurganus, Dustin; Cassarly, William J.; Davies, Matthew A.; Suleski, Thomas J. (January 2022, Optics Express)

   Dynamic illumination using tunable freeform arrays can enable spatial light distributions of variable size with high uniformity from non-uniform sources through relatively small opposing lateral shifts applied to the freeform components. We present the design, manufacturing, and characterization of a tunable LED-based illuminator using custom freeform Alvarez arrays with commercially available optics to shorten the manufacturing cycle. The optomechanical design and manufacturing of the Alvarez lens arrays and mounting parts are presented in detail. The optical performance of the system is evaluated and compared with simulation results using a custom camera-based test station. Experimental results demonstrate and confirm the dynamic illumination concept with good uniformity. 

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3. Telecentric broadband objective lenses for optical coherence tomography (OCT) in the context of low uncertainty metrology of freeform optical components: from design to testing for wavefront and telecentricity

   https://doi.org/10.1364/OE.27.006184  

   Xu, D; Chaudhuri, R; Rolland, J (March 2019, Optics express)

   Freeform optical components enable significant advances for optical systems. A major challenge for freeform optics is the current lack of metrology methods with measurement uncertainty on the order of tens of nanometers or less. Towards addressing this challenge, optical coherence tomography (OCT) is a viable technique. In the context of low uncertainty metrology, the design requirements pertaining to the sample arm of an OCT metrology system are explicitly addressed in this paper. Two telecentric, broadband, diffraction limited, custom objective lens designs are presented with their design strategies. One objective lens was fabricated and experimentally tested for wavefront performance and telecentricity. This lens demonstrates near diffraction limited performance and a maximum deviation from telecentricity of 8.7 arcseconds across the full field of view, correlating to measurement uncertainty of less than 12 nm in simulation. The telecentricity test method developed completes the loop with respect to the design requirements and strategies presented and provides further intuition for telecentric lens designs in general. © 2019 Optical Society of America under the terms of the OSA Open Access Publishing Agreement 

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4. Freeform Illuminator for Computational Microscopy

   https://doi.org/10.34133/icomputing.0015  

   Song, Pengming; Wang, Tianbo; Jiang, Shaowei; Guo, Chengfei; Wang, Ruihai; Yang, Liming; Zhou, You; Zheng, Guoan (January 2023, Intelligent Computing)

   Programmable illumination control is essential for many computational microscopy techniques. Conventional light source array is often arranged on a fixed grid of a planar surface for providing programmable sample illumination. Here, we report the development of a freeform illuminator that can be arranged at arbitrary 2-dimensional or 3-dimensional (3D) surface structures for computational microscopy. The freeform illuminator can be designed in a small form factor with a dense light source arrangement in 3D. It can be placed closer to the sample for providing angle-varied illumination with higher optical flux and smaller angular increment. With the freeform illuminators, we develop a calibration process using a low-cost Raspberry-Pi image sensor coated with a monolayer of blood cells. By tracking the positional shift of the blood-cell diffraction patterns at 2 distinct regions of the coded sensor, we can infer the 3D positions of the light source elements in a way similar to the stereo vision reconstruction approach. To demonstrate the applications for computational microscopy, we validate the freeform illuminators for Fourier ptychographic microscopy, 3D tomographic imaging, and on-chip microscopy. We also present a longitudinal study by tracking the growth of live bacterial cultures over a large field of view. The reported freeform illuminators and the related calibration process offer flexibilities and extended scope for imaging innovations in computational microscopy. 

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5. All-reflective freeform microscope objective for ultra-broadband microscopy

   https://doi.org/10.1364/OE.544492  

   Bauer, Aaron; Rolland, Jannick P; Clark, Stephan; Potma, Eric; Hanninen, Adam (December 2024, Optics Express)

   Conventional refractive microscope objective lenses have limited applicability to a range of imaging modalities due to the dispersive nature of their optical elements. Designing a conventional refractive microscope objective that provides well-corrected imaging over a broad spectral range can be challenging, if not impossible. In contrast, reflective optics are inherently achromatic, so a system composed entirely of reflective elements is free from chromatic aberrations and, as a result, can image over an ultra-wide spectral range with perfect color correction. This study explores the design space of unobscured high numerical aperture, all-reflective microscope objectives. In particular, using freeform optical elements we obviate the need for a center obscuration, rendering the objective’s modulation transfer function comparable to that of refractive lens systems of similar numerical aperture. We detail the design process of the reflective objective, from determining the design specifications to the system optimization and sensitivity analysis. The outcome is an all-reflective freeform microscope objective lens with a 0.65 numerical aperture that provides diffraction-limited imaging and is compatible with the geometric constraints of commercial microscope systems. 

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