RationaleIt is imperative to understand how chemical preservation alters tissue isotopic compositions before using historical samples in ecological studies. Specifically, although compound‐specific isotope analysis of amino acids (CSIA‐AA) is becoming a widely used tool, there is little information on how preservation techniques affect amino acidδ15N values. MethodsWe evaluated the effects of chemical preservatives on bulk tissueδ13C andδ15N and amino acidδ15N values, measured by gas chromatography/isotope ratio mass spectrometry (GC/IRMS), of (a) tuna (Thunnus albacares) and squid (Dosidicus gigas) muscle tissues that were fixed in formaldehyde and stored in ethanol for 2 years and (b) two copepod species,Calanus pacificusandEucalanus californicus, which were preserved in formaldehyde for 24–25 years. ResultsTissues in formaldehyde‐ethanol had higher bulkδ15N values (+1.4,D. gigas; +1.6‰,T. albacares), higherδ13C values forD. gigas(+0.5‰), and lowerδ13C values forT. albacares(−0.8‰) than frozen samples. The bulkδ15N values from copepods were not different those from frozen samples, although theδ13C values from both species were lower (−1.0‰ forE. californicusand −2.2‰ forC. pacificus) than those from frozen samples. The mean amino acidδ15N values from chemically preserved tissues were largely within 1‰ of those of frozen tissues, but the phenylalanineδ15N values were altered to a larger extent (range: 0.5–4.5‰). ConclusionsThe effects of preservation on bulkδ13C values were variable, where the direction and magnitude of change varied among taxa. The changes in bulkδ15N values associated with chemical preservation were mostly minimal, suggesting that storage in formaldehyde or ethanol will not affect the interpretation ofδ15N values used in ecological studies. The preservation effects on amino acidδ15N values were also mostly minimal, mirroring bulkδ15N trends, which is promising for future CSIA‐AA studies of archived specimens. However, there were substantial differences in phenylalanine and valineδ15N values, which we speculate resulted from interference in the chromatographic resolution of unknown compounds rather than alteration of tissue isotopic composition due to chemical preservation. 
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                            Radiocarbon Dating of Silica Sinter and Postglacial Hydrothermal Activity in the El Tatio Geyser Field
                        
                    
    
            Abstract The El Tatio geothermal field in the Chilean Altiplano contains hydrothermal silica sinter deposits overlaying glacial and volcanic units, providing an opportunity to constrain the timing of deglaciation and volcanic activity in an area with sparse absolute chronologies. We obtained 51 new radiocarbon ages andδ13C values on the organic material trapped in these sinter deposits. Based on the δ13C values, we exclude 29 samples for possible contamination with bacterial mats that incorporate old carbon. We infer that hydrothermal activity initiated ~27 ka ago and has been nearly continuous ever since. The ages of the oldest sinter deposits coincide with ages of moraines that stabilized after the most recent deglaciation. Whereas late Pleistocene sinters are broadly distributed in the field, Holocene deposits are found around active hydrothermal features. Although recent volcanism is absent in the vicinity of El Tatio, persistent hydrothermal discharge implies a long‐lived magmatic heat source. 
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                            - Award ID(s):
- 1724986
- PAR ID:
- 10455338
- Publisher / Repository:
- DOI PREFIX: 10.1029
- Date Published:
- Journal Name:
- Geophysical Research Letters
- Volume:
- 47
- Issue:
- 11
- ISSN:
- 0094-8276
- Format(s):
- Medium: X
- Sponsoring Org:
- National Science Foundation
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