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Title: Highly Abundant Proteins Are Highly Thermostable
Highly abundant proteins tend to evolve slowly (a trend called E-R anticorrelation), and a number of hypotheses have been proposed to explain this phenomenon. The misfolding avoidance hypothesis attributes the E-R anticorrelation to the abundance-dependent toxic effects of protein misfolding. To avoid these toxic effects, protein sequences (particularly those of highly expressed proteins) would be under selection to fold properly. One prediction of the misfolding avoidance hypothesis is that highly abundant proteins should exhibit high thermostability (i.e., a highly negative free energy of folding, ΔG). Thus far, only a handful of analyses have tested for a relationship between protein abundance and thermostability, producing contradictory results. These analyses have been limited by 1) the scarcity of ΔG data, 2) the fact that these data have been obtained by different laboratories and under different experimental conditions, 3) the problems associated with using proteins’ melting energy (Tm) as a proxy for ΔG, and 4) the difficulty of controlling for potentially confounding variables. Here, we use computational methods to compare the free energy of folding of pairs of human–mouse orthologous proteins with different expression levels. Even though the effect size is limited, the most highly expressed ortholog is often the one with a more negative ΔG of folding, indicating that highly expressed proteins are often more thermostable.  more » « less
Award ID(s):
1817413 1818288
PAR ID:
10475908
Author(s) / Creator(s):
; ; ; ; ;
Editor(s):
dos Reis, Mario
Publisher / Repository:
Genome Biology and Evolution
Date Published:
Journal Name:
Genome Biology and Evolution
Volume:
15
Issue:
7
ISSN:
1759-6653
Format(s):
Medium: X
Sponsoring Org:
National Science Foundation
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