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Title: ELISA Assay with PDMS Microfluidic Channels Fabricated by 3D Printed Master Mold
Following the emergence of the SARS-CoV-2 (Covid-19) pandemic, interest in understanding antibody diagnostic testing has increased. We describe a quick and inexpensive technique that enabled students to print their own microfluidic devices that can be used to house an immunoassay for detecting a Human Immunodeficiency Virus (HIV) antibody. Both qualitative diagnostic assays and quantitative binding assays were carried out to characterize the HIV interaction with a target antibody. By performing these hands-on low-cost experiments in the analytical chemistry lab course, students were exposed to 3D fabrication, microfluidic technology, surface chemistry, protein-ligand binding affinity studies, and immunoassays within the time frame of two four–hour laboratory periods.  more » « less
Award ID(s):
2004050 2305129
PAR ID:
10489401
Author(s) / Creator(s):
Publisher / Repository:
Springer
Date Published:
Journal Name:
The Chemical educator
ISSN:
1430-4171
Format(s):
Medium: X
Sponsoring Org:
National Science Foundation
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