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1. Drone‐based imaging sensors, techniques, and applications in plant phenotyping for crop breeding: A comprehensive review

   https://doi.org/10.1002/ppj2.20100  

   Gano, Boubacar; Bhadra, Sourav; Vilbig, Justin M; Ahmed, Nurzaman; Sagan, Vasit; Shakoor, Nadia (December 2024, The Plant Phenome Journal)

   Over the last decade, the use of unmanned aerial vehicles (UAVs) for plant phenotyping and field crop monitoring has significantly evolved and expanded. These technologies have been particularly valuable for monitoring crop growth and health and for managing abiotic and biotic stresses such as drought, fertilization deficiencies, disease, and bioaggressors. This paper provides a comprehensive review of the progress in UAV‐based plant phenotyping, with a focus on the current use and application of drone technology to gain information on plant growth, development, adaptation, and yield. We reviewed over 200 research articles and discuss the best tools and methodologies for different research purposes, the challenges that need to be overcome, and the major research gaps that remain. First, the review offers a critical focus on elucidating the distinct characteristics of UAV platforms, highlighting the diverse sensor technologies employed and shedding light on the nuances of UAV data acquisition and processing methodologies. Second, it presents a comprehensive analysis of the multiple applications of UAVs in field phenotyping, underscoring the transformative potential of integrating machine learning techniques for plant analysis. Third, it delves into the realm of machine learning applications for plant phenotyping, emphasizing its role in enhancing data analysis and interpretation. Furthermore, the paper extensively examines the open issues and research challenges within the domain, addressing the complexities and limitations faced during data acquisition, processing, and interpretation. Finally, it outlines the future trends and emerging technologies in the field of UAV‐based plant phenotyping, paving the way for innovative advancements and methodologies. 

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2. Integrating Automated Labeling Framework for Enhancing Deep Learning Models to Count Corn Plants Using UAS Imagery

   https://doi.org/10.3390/s24196467  

   Katari, Sushma; Venkatesh, Sandeep; Stewart, Christopher; Khanal, Sami (October 2024, Sensors)

   Plant counting is a critical aspect of crop management, providing farmers with valuable insights into seed germination success and within-field variation in crop population density, both of which are key indicators of crop yield and quality. Recent advancements in Unmanned Aerial System (UAS) technology, coupled with deep learning techniques, have facilitated the development of automated plant counting methods. Various computer vision models based on UAS images are available for detecting and classifying crop plants. However, their accuracy relies largely on the availability of substantial manually labeled training datasets. The objective of this study was to develop a robust corn counting model by developing and integrating an automatic image annotation framework. This study used high-spatial-resolution images collected with a DJI Mavic Pro 2 at the V2–V4 growth stage of corn plants from a field in Wooster, Ohio. The automated image annotation process involved extracting corn rows and applying image enhancement techniques to automatically annotate images as either corn or non-corn, resulting in 80% accuracy in identifying corn plants. The accuracy of corn stand identification was further improved by training four deep learning (DL) models, including InceptionV3, VGG16, VGG19, and Vision Transformer (ViT), with annotated images across various datasets. Notably, VGG16 outperformed the other three models, achieving an F1 score of 0.955. When the corn counts were compared to ground truth data across five test regions, VGG achieved an R2 of 0.94 and an RMSE of 9.95. The integration of an automated image annotation process into the training of the DL models provided notable benefits in terms of model scaling and consistency. The developed framework can efficiently manage large-scale data generation, streamlining the process for the rapid development and deployment of corn counting DL models. 

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3. Retrieval of Crop Canopy Chlorophyll: Machine Learning vs. Radiative Transfer Model

   https://doi.org/10.3390/rs16122058  

   Alam, Mir_Md Tasnim; Simic_Milas, Anita; Gašparović, Mateo; Osei, Henry Poku (June 2024, Remote Sensing)

   In recent years, the utilization of machine learning algorithms and advancements in unmanned aerial vehicle (UAV) technology have caused significant shifts in remote sensing practices. In particular, the integration of machine learning with physical models and their application in UAV–satellite data fusion have emerged as two prominent approaches for the estimation of vegetation biochemistry. This study evaluates the performance of five machine learning regression algorithms (MLRAs) for the mapping of crop canopy chlorophyll at the Kellogg Biological Station (KBS) in Michigan, USA, across three scenarios: (1) application to Landsat 7, RapidEye, and PlanetScope satellite images; (2) application to UAV–satellite data fusion; and (3) integration with the PROSAIL radiative transfer model (hybrid methods PROSAIL + MLRAs). The results indicate that the majority of the five MLRAs utilized in UAV–satellite data fusion perform better than the five PROSAIL + MLRAs. The general trend suggests that the integration of satellite data with UAV-derived information, including the normalized difference red-edge index (NDRE), canopy height model, and leaf area index (LAI), significantly enhances the performance of MLRAs. The UAV–RapidEye dataset exhibits the highest coefficient of determination (R2) and the lowest root mean square errors (RMSE) when employing kernel ridge regression (KRR) and Gaussian process regression (GPR) (R2 = 0.89 and 0.89 and RMSE = 8.99 µg/cm2 and 9.65 µg/cm2, respectively). Similar performance is observed for the UAV–Landsat and UAV–PlanetScope datasets (R2 = 0.86 and 0.87 for KRR, respectively). For the hybrid models, the maximum performance is attained with the Landsat data using KRR and GPR (R2 = 0.77 and 0.51 and RMSE = 33.10 µg/cm2 and 42.91 µg/cm2, respectively), followed by R2 = 0.75 and RMSE = 39.78 µg/cm2 for the PlanetScope data upon integrating partial least squares regression (PLSR) into the hybrid model. Across all hybrid models, the RapidEye data yield the most stable performance, with the R2 ranging from 0.45 to 0.71 and RMSE ranging from 19.16 µg/cm2 to 33.07 µg/cm2. The study highlights the importance of synergizing UAV and satellite data, which enables the effective monitoring of canopy chlorophyll in small agricultural lands. 

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4. Influence of soil heterogeneity on soybean plant development and crop yield evaluated using time-series of UAV and ground-based geophysical imagery

   https://doi.org/10.1038/s41598-021-86480-z  

   Falco, Nicola; Wainwright, Haruko M.; Dafflon, Baptiste; Ulrich, Craig; Soom, Florian; Peterson, John E.; Brown, James Bentley; Schaettle, Karl B.; Williamson, Malcolm; Cothren, Jackson D.; et al (December 2021, Scientific Reports)

   Abstract Understanding the interactions among agricultural processes, soil, and plants is necessary for optimizing crop yield and productivity. This study focuses on developing effective monitoring and analysis methodologies that estimate key soil and plant properties. These methodologies include data acquisition and processing approaches that use unmanned aerial vehicles (UAVs) and surface geophysical techniques. In particular, we applied these approaches to a soybean farm in Arkansas to characterize the soil–plant coupled spatial and temporal heterogeneity, as well as to identify key environmental factors that influence plant growth and yield. UAV-based multitemporal acquisition of high-resolution RGB (red–green–blue) imagery and direct measurements were used to monitor plant height and photosynthetic activity. We present an algorithm that efficiently exploits the high-resolution UAV images to estimate plant spatial abundance and plant vigor throughout the growing season. Such plant characterization is extremely important for the identification of anomalous areas, providing easily interpretable information that can be used to guide near-real-time farming decisions. Additionally, high-resolution multitemporal surface geophysical measurements of apparent soil electrical conductivity were used to estimate the spatial heterogeneity of soil texture. By integrating the multiscale multitype soil and plant datasets, we identified the spatiotemporal co-variance between soil properties and plant development and yield. Our novel approach for early season monitoring of plant spatial abundance identified areas of low productivity controlled by soil clay content, while temporal analysis of geophysical data showed the impact of soil moisture and irrigation practice (controlled by topography) on plant dynamics. Our study demonstrates the effective coupling of UAV data products with geophysical data to extract critical information for farm management. 

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5. Predicting Table Beet Root Yield with Multispectral UAS Imagery

   https://doi.org/10.3390/rs13112180  

   Chancia, Robert; van Aardt, Jan; Pethybridge, Sarah; Cross, Daniel; Henderson, John (June 2021, Remote Sensing)

   null (Ed.)

   Timely and accurate monitoring has the potential to streamline crop management, harvest planning, and processing in the growing table beet industry of New York state. We used unmanned aerial system (UAS) combined with a multispectral imager to monitor table beet (Beta vulgaris ssp. vulgaris) canopies in New York during the 2018 and 2019 growing seasons. We assessed the optimal pairing of a reflectance band or vegetation index with canopy area to predict table beet yield components of small sample plots using leave-one-out cross-validation. The most promising models were for table beet root count and mass using imagery taken during emergence and canopy closure, respectively. We created augmented plots, composed of random combinations of the study plots, to further exploit the importance of early canopy growth area. We achieved a R2 = 0.70 and root mean squared error (RMSE) of 84 roots (~24%) for root count, using 2018 emergence imagery. The same model resulted in a RMSE of 127 roots (~35%) when tested on the unseen 2019 data. Harvested root mass was best modeled with canopy closing imagery, with a R2 = 0.89 and RMSE = 6700 kg/ha using 2018 data. We applied the model to the 2019 full-field imagery and found an average yield of 41,000 kg/ha (~40,000 kg/ha average for upstate New York). This study demonstrates the potential for table beet yield models using a combination of radiometric and canopy structure data obtained at early growth stages. Additional imagery of these early growth stages is vital to develop a robust and generalized model of table beet root yield that can handle imagery captured at slightly different growth stages between seasons. 

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