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  • Genome-scale flux balance analysis reveals redox trade-offs in the metabolism of the thermoacidophile Methylacidiphilum fumariolicum under auto-, hetero-and methanotrophic conditions
Title: Genome-scale flux balance analysis reveals redox trade-offs in the metabolism of the thermoacidophile Methylacidiphilum fumariolicum under auto-, hetero-and methanotrophic conditions
Members of the genusMethylacidiphilumare thermoacidophile methanotrophs with optimal growth temperatures between 50°C and 60°C, and pH between 1.0 and 3.0. These microorganisms, as well as other extremophile bacteria, offer an attractive platform for environmental and industrial biotechnology because of their robust operating conditions and capacity to grow using low-cost substrates. In this study, we isolatedMethylacidiphilum fumariolicumstr. Pic from a crater lake located in the state of Chiapas, Mexico. We sequenced the genome and built a genome-scale metabolic model. The manually curated model contains 667 metabolites, 729 reactions, and 473 genes. Predicted flux distributions using flux balance analysis identified changes in redox trade-offs under methanotrophic and autotrophic conditions (H2+CO2). This was also predicted under heterotrophic conditions (acetone, isopropanol, and propane). Model validation was performed by testing the capacity of the strains to grow using four substrates: CH4, acetone, isopropanol, and LP-Gas. The results suggest that the metabolism ofM. fumariolicumstr. Pic is limited by the regeneration of redox equivalents such as NAD(P)H and reduced cytochromes.  more » « less
Award ID(s):
2313313
PAR ID:
10533102
Author(s) / Creator(s):
; ; ;
Corporate Creator(s):
Editor(s):
Rodrigue, Agnès
Publisher / Repository:
Frontiers
Date Published:
Journal Name:
Frontiers in Systems Biology
Edition / Version:
1
Volume:
4
Issue:
1
ISSN:
2674-0702
Page Range / eLocation ID:
1-18
Subject(s) / Keyword(s):
verrucomicrobia, thermoacidophile, methanotroph, genome-scale metabolic model, flux balance analysis
Format(s):
Medium: X Size: 2MB Other: xls
Size(s):
2MB
Sponsoring Org:
National Science Foundation
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