Title: Draft genome of Bacillus subtilis strain YB955, prophage-cured derivative of strain 168
ABSTRACT We report the genome sequence ofBacillus subtilisstrain YB955, a prophage-cured strain used as a model in DNA repair, bacterial physiology, and mutagenesis studies. The assembled and annotated draft genome contains 4,031 coding genes, 5 rRNAs, and 73 tRNAs. Compared to 168, YB955 has a 134,402 bp deletion. more »« less
Chase, Emily E; Truchon, Alexander R; Schepens, William W; Wilhelm, Steven W
(, Microbiology Resource Announcements)
Hudson, André O
(Ed.)
ABSTRACT Here, we report the draft genome ofAureococcus anophagefferensstrain CCMP1851, which is susceptible to the virusKratosvirus quantuckense. CCMP1851 complements an available genome for a virus-resistant strain (CCMP1850) isolated from the same bloom. Future studies can now use this genome to examine genetic hints of virus resistance and susceptibility.
Farrell, Morgan V; Airkin, Mina Y; Ali, Tatyana N; Altoblani, Zainalabdin S; Bowman, Chynna R; Diaz, Abigail_Anne B; Faurot, Paul F; Frausto, Joshua E; Haji, Sazan F; Hamad, Basma A; et al
(, Microbiology Resource Announcements)
Stewart, Frank J
(Ed.)
ABSTRACT Here, we report the draft genome sequence ofExiguobacteriumsp. strain MMG028, isolated from Rose Creek, San Diego, CA, USA, assembled and analyzed by undergraduate students participating in a marine microbial genomics course. A genomic comparison suggests that MMG028 is a novel species, providing a resource for future microbiology and biotechnology investigations.
Elkassas, Sabrina M; Serres, Margrethe H; Richardson, Derrick; Zhilina, Tatyana N; Huber, Julie A
(, Microbiology Resource Announcements)
Roux, Simon
(Ed.)
ABSTRACT A methanogenic archaeon was isolated from bottom sediments in the vicinity of Lake Tanatar II (Altai, Russia), an alkaline soda lake. Here we present the draft genome sequence ofMethanocalculus natronophilussp. strain Z-7105T.
Stajich, Jason E; Garvey, Sean M; Gil-Serna, Jéssica
(, Microbiology Resource Announcements)
Pritchard, Leighton
(Ed.)
ABSTRACT The fungusAspergillus melleusis an important biosynthesis host for varied commercial applications. Gene annotation of a previously published genome produced 12,841 protein-coding genes and identified 102 biosynthetic gene clusters.
ABSTRACT Bacillus subtilisis an important industrial and environmental microorganism known to occupy many niches and produce many compounds of interest. Although it is one of the best-studied organisms, much of this focus including the reconstruction of genome-scale metabolic models has been placed on a few key laboratory strains. Here, we substantially expand these prior models to pan-genome-scale, representing 481 genomes ofB. subtiliswith 2,315 orthologous gene clusters, 1,874 metabolites, and 2,239 reactions. Furthermore, we incorporate data from carbon utilization experiments for eight strains to refine and validate its metabolic predictions. This comprehensive pan-genome model enables the assessment of strain-to-strain differences related to nutrient utilization, fermentation outputs, robustness, and other metabolic aspects. Using the model and phenotypic predictions, we divideB. subtilisstrains into five groups with distinct patterns of behavior that correlate across these features. The pan-genome model offers deep insights intoB. subtilis’metabolism as it varies across environments and provides an understanding as to how different strains have adapted to dynamic habitats. IMPORTANCEAs the volume of genomic data and computational power have increased, so has the number of genome-scale metabolic models. These models encapsulate the totality of metabolic functions for a given organism.Bacillus subtilisstrain 168 is one of the first bacteria for which a metabolic network was reconstructed. Since then, several updated reconstructions have been generated for this model microorganism. Here, we expand the metabolic model for a single strain into a pan-genome-scale model, which consists of individual models for 481B. subtilisstrains. By evaluating differences between these strains, we identified five distinct groups of strains, allowing for the rapid classification of any particular strain. Furthermore, this classification into five groups aids the rapid identification of suitable strains for any application.
Perez, Ryan King, Chavez_Rios, Jocelyn Sofia, Grifaldo, Jessica, Regner, Kurt, Pedraza-Reyes, Mario, and Robleto, Eduardo A.
"Draft genome of Bacillus subtilis strain YB955, prophage-cured derivative of strain 168". Microbiology Resource Announcements 13 (8). Country unknown/Code not available: American Society for Microbiology. https://doi.org/10.1128/mra.00263-24.https://par.nsf.gov/biblio/10540789.
@article{osti_10540789,
place = {Country unknown/Code not available},
title = {Draft genome of Bacillus subtilis strain YB955, prophage-cured derivative of strain 168},
url = {https://par.nsf.gov/biblio/10540789},
DOI = {10.1128/mra.00263-24},
abstractNote = {ABSTRACT We report the genome sequence ofBacillus subtilisstrain YB955, a prophage-cured strain used as a model in DNA repair, bacterial physiology, and mutagenesis studies. The assembled and annotated draft genome contains 4,031 coding genes, 5 rRNAs, and 73 tRNAs. Compared to 168, YB955 has a 134,402 bp deletion.},
journal = {Microbiology Resource Announcements},
volume = {13},
number = {8},
publisher = {American Society for Microbiology},
author = {Perez, Ryan King and Chavez_Rios, Jocelyn Sofia and Grifaldo, Jessica and Regner, Kurt and Pedraza-Reyes, Mario and Robleto, Eduardo A},
editor = {Roux, Simon}
}
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