Title: Cannabis pollen dispersal across the United States
For the recently legalized US hemp industry (Cannabis sativa), cross-pollination between neighboring fields has become a significant challenge, leading to contaminated seeds, reduced oil yields, and in some cases, mandated crop destruction. As a step towards assessing hemp cross-pollination risk, this study characterizes the seasonal and spatial patterns in windborne hemp pollen dispersal spanning the conterminous United States (CONUS). By leveraging meteorological data obtained through mesoscale model simulations, we have driven Lagrangian Stochastic models to simulate wind-borne hemp pollen dispersion across CONUS on a county-by-county basis for five months from July to November, encompassing the potential flowering season for industrial hemp. Our findings reveal that pollen deposition rates escalate from summer to autumn due to the reduction in convective activity during daytime and the increase in wind shear at night as the season progresses. We find diurnal variations in pollen dispersion: nighttime conditions favor deposition in proximity to the source, while daytime conditions facilitate broader dispersal albeit with reduced deposition rates. These shifting weather patterns give rise to specific regions of CONUS more vulnerable to hemp cross-pollination.  more » « less
Award ID(s):
1922516
PAR ID:
10571303
Author(s) / Creator(s):
; ;
Publisher / Repository:
Nature Portfolio
Date Published:
Journal Name:
Scientific Reports
Volume:
14
Issue:
1
ISSN:
2045-2322
Subject(s) / Keyword(s):
Cannabis pollen dispersal Hemp cross-pollination pollen cross-pollination Windborne pollen transport Lagrangian Stochastic models LS models Mesoscale meteorological modeling Nonlinear dispersion modeling Convective and shear-driven turbulence Diurnal and seasonal pollen transport Environmental impact of hemp cultivation Agricultural risk assessment cross-pollination vulnerability risk assessment
Format(s):
Medium: X
Sponsoring Org:
National Science Foundation
More Like this
  1. Abstract Premise Many tropical plants are bat‐pollinated, but these mammals often carry copious, multispecific pollen loads making bat‐pollinated plants susceptible to heterospecific pollen deposition and reproductive interference. We investigated pollen transfer between sympatric bat‐pollinated Burmeistera species and their response to heterospecific pollen deposition from each other. Methods We quantified conspecific and heterospecific pollen deposition for two populations of B. ceratocarpa , a recipient species in heterospecific pollen transfer interactions, that co‐occur with different donor relatives ( B. borjensis and B. glabrata ). We then used a cross‐pollination scheme using pollen mixtures to assess the species' responses to heterospecific pollen deposition in terms of fruit abortion and seed production. Results Burmeistera ceratocarpa received significantly more heterospecific pollen from its relatives at both sites than its own pollen was deposited on its relatives. However, heterospecific pollen deposition only affected seed production by B. borjensis and B. glabrata , but not by B. ceratocarpa , suggesting that early acting post‐pollination barriers buffer the latter against reproductive interference. Crosses between sympatric and allopatric populations suggest that the study species are fully isolated in sympatry, while isolation between allopatric populations is strong but incomplete. Conclusions We did not observe evidence of reproductive interference among our study species, because either heterospecific pollen deposition did not affect their seed production ( B. ceratocarpa ) or they receive heterospecific pollen only rarely ( B. borjensis and B. glabrata ). Frequent heterospecific pollen deposition might favor the evolution of barriers against foreign pollen (as in B. ceratocarpa ) that alleviate the competitive costs of sharing low fidelity pollinators with co‐occurring species. 
    more » « less
  2. Flowering time in Alaskan boreal forest is advancing, and this may affect pollination rates of early-flowering species. Viburnum edule is one of the first understory plants to flower, when pollinator diversity and abundance are likely lower than later in the season.  We evaluated the impact of flowering time on pollen deposition and composition of the pollinator community over two years (one in which plants flowered slightly earlier than average and one in which flowering time was close to average) using experimental arrays with branches that flowered either at the start or the peak of flowering for each year. Pollinator exclusion reduced fruit set by > 90%, but even plants freely pollinated by insects had fruit set rates of < 10%. Both within and across years, plants that flowered later had more insect visitors and higher proportions of stigmas visited (> 5 pollen grains per stigma); in the advanced year, plants that flowered later also had more pollen grains per stigma. Syrphid flies, solitary bees, and muscid flies constituted ~ 99% of visitors, with a higher proportion of syrphid flies for later-flowering plants within and across years. Despite evidence for potential pollen limitation, pollen loads for the earliest flowering plants were high (mean > 25 pollen grains per stigma). Fruit production in V. edule is likely limited by inefficient pollen transfer between genets, by resource availability, or both. Given the prevalence of syrphid and muscid flies as pollinators, we need a better understanding of what triggers emergence in these taxa to evaluate the potential for trophic mismatches in boreal forest. 
    more » « less
  3. Dioecious plants are obligate outcrossers with separate male and female individuals, which can result in decreased seed set with increasing distance between the sexes. Wind pollination is a common correlate of dioecy, yet combined wind and insect pollination (ambophily) could be advantageous in compensating for decreased pollen flow to isolated females. Dioecious, ambophilous gymnospermsEphedra(Gnetales) secrete pollination drops (PDs) in female cones that capture airborne pollen and attract ants that feed on them. Plant sugary secretions commonly reward ants in exchange for indirect plant defense against herbivores, and more rarely for pollination. We conducted field experiments to investigate whether ants are pollinators and/or plant defenders of South AmericanEphedra triandra, and whether their contribution to seed set and seed cone protection varies with distance between female and male plants. We quantified pollen flow in the wind and assessed the effectiveness of ants as pollinators by investigating their relative contribution to seed set, and their visitation rate in female plants at increasing distance from the nearest male. Ants accounted for most insect visits to female cones ofE. triandra, where they consumed PDs, and pollen load was larger on bigger ants without reduction in pollen viability. While wind pollination was the main contributor to seed set overall, the relative contribution of ants was distance dependent. Ant contribution to seed set was not significant at shorter distances, yet at the farthest distance from the nearest male (23 m), where 20 times less pollen reached females, ants enhanced seed set by 30% compared to plants depending solely on wind pollination. We found no evidence that ants contribute to plant defense by preventing seed cone damage. Our results suggest that, despite their short-range movements, ants can offset pollen limitation in isolated females of wind-pollinated plants with separate sexes. We propose that ants enhance plant reproductive success via targeted delivery of airborne pollen, through frequent contact with ovule tips while consuming PDs. Our study constitutes the first experimental quantification of distance-dependent contribution of ants to pollination and provides a working hypothesis for ambophily in other dioecious plants lacking pollinator reward in male plants. 
    more » « less
  4. Abstract Background Siring success plays a key role in plant evolution and reproductive ecology, and variation among individuals creates an opportunity for selection to act. Differences in male reproductive success can be caused by processes that occur during two stages, the pollination and post-pollination phases of reproduction. In the pollination phase, heritable variation in floral traits and floral display affect pollinator visitation patterns, which in turn affect variation among plants in the amount of pollen exported and deposited on recipient stigmas. In the post-pollination phase, differences among individuals in pollen grain germination success and pollen tube growth may cause realized paternity to differ from patterns of pollen receipt. The maternal plant can also preferentially provision some developing seeds or fruits to further alter variation in siring success. Scope In this review, we describe studies that advance our understanding of the dynamics of the pollination and post-pollination phases, focusing on how variation in male fitness changes in response to pollen limitation. We then explore the interplay between pollination and post-pollination success, and how these processes respond to ecological factors such as pollination intensity. We also identify pressing questions at the intersection of pollination and paternity and describe novel experimental approaches to elucidate the relative importance of pollination and post-pollination factors in determining male reproductive success. Conclusions The relative contribution of pollination and post-pollination processes to variation in male reproductive success may not be constant, but rather may vary with pollination intensity. Studies that quantify the effects of pollination and post-pollination phases in concert will be especially valuable as they will enable researchers to more fully understand the ecological conditions influencing male reproductive success. 
    more » « less
  5. The Great Plains (GP) low-level jet (GPLLJ) contributes to GP warm season water resources (precipitation), wind resources, and severe weather outbreaks. Past research has shown that synoptic and local mesoscale physical mechanisms (Holton and Blackadar mechanisms) are required to explain GPLLJ variability. Although soil moisture–PBL interactions are central to local mechanistic theories, the diurnal effect of regional soil moisture anomalies on GPLLJ speed, northward penetration, and propensity for severe weather is not well known. In this study, two 31-member WRF-ARW stochastic kinetic energy backscatter scheme ensembles simulate a typical warm season GPLLJ case under CONUS-wide wet and dry soil moisture scenarios. In the GP (24°–48°N, 103°–90°W), ensemble mean differences in sensible heating and PBL height of 25–150 W m −2 and 100–700 m, respectively, at 2100 UTC (afternoon) culminate in GPLLJ 850-hPa wind speed differences of 1–4 m s −1 12 hours later (0900 UTC; early morning). Greater heat accumulation in the daytime PBL over dry soil impacts the east–west geopotential height gradient in the GP (synoptic conditions and Holton mechanism) resulting in a deeper thermal low in the northern GP, causing increases in the geostrophic wind. Enhanced daytime turbulent mixing over dry soil impacts the PBL structure (Blackadar mechanism), leading to increased ageostrophic wind. Overnight geostrophic and ageostrophic winds constructively interact, leading to a faster nocturnal GPLLJ over dry soil. Ensemble differences in CIN (~50–150 J kg −1 ) and CAPE (~500–1000 J kg −1 ) have implications for severe weather predictability. 
    more » « less