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SUMMARY Floral nectar is a sugary solution produced by nectaries to attract and reward pollinators. Nectar metabolites, such as sugars, are synthesized within the nectary during secretion from both pre‐stored and direct phloem‐derived precursors. In addition to sugars, nectars contain nitrogenous compounds such as amino acids; however, little is known about the role(s) of nitrogen (N) compounds in nectary function. In this study, we investigated N metabolism inCucurbita pepo(squash) floral nectaries in order to understand how various N‐containing compounds are produced and determine the role of N metabolism in nectar secretion. The expression and activity of key enzymes involved in primary N assimilation, including nitrate reductase (NR) and alanine aminotransferase (AlaAT), were induced during secretion inC. peponectaries. Alanine (Ala) accumulated to about 35% of total amino acids in nectaries and nectar during peak secretion; however, alteration of vascular nitrate supply had no impact on Ala accumulation during secretion, suggesting that nectar(y) amino acids are produced by precursors other than nitrate. In addition, nitric oxide (NO) is produced from nitrate and nitrite, at least partially by NR, in nectaries and nectar. Hypoxia‐related processes are induced in nectaries during secretion, including lactic acid and ethanolic fermentation. Finally, treatments that alter nitrate supply affect levels of hypoxic metabolites, nectar volume and nectar sugar composition. The induction of N metabolism inC. peponectaries thus plays an important role in the synthesis and secretion of nectar sugar.
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The developmental basis of floral nectary diversity and evolution
Summary Nectar is a central bridge between angiosperms and animal mutualists. It is produced by specialized structures termed nectaries, which can be found on different plant organs. Consumption of floral nectar by pollinators and the subsequent transfer of pollen contribute to the reproductive success of both angiosperms and their pollinators. Floral nectaries have evolved many times independently, feature diverse structural organizations, and produce nectars with various compositions, which cater to a wide range of pollinators. While the nectary and its nectar have been documented for two millennia, many aspects of nectary biology are still unknown. Recent advances in genetics, genomics, and comparative analyses across diverse species have accelerated our understanding of floral nectary structures and the genetic circuits behind their formation and evolution. In this review, we summarize the recent breakthroughs in nectary research and provide a macroevolutionary framework of floral nectary evolution, focusing on the genetic mechanisms that drive nectary development and shape nectary diversity.
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- Award ID(s):
- 2305493
- PAR ID:
- 10587384
- Publisher / Repository:
- Wiley-Blackwell
- Date Published:
- Journal Name:
- New Phytologist
- Volume:
- 246
- Issue:
- 6
- ISSN:
- 0028-646X
- Format(s):
- Medium: X Size: p. 2462-2477
- Size(s):
- p. 2462-2477
- Sponsoring Org:
- National Science Foundation
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