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Blooms of planktonic cyanobacteria have long been of concern in lakes, but more recently, harmful impacts of riverine benthic cyanobacterial mats been recognized. As yet, we know little about how various benthic cyanobacteria are distributed in river networks, or how environmental conditions or other associated microbes in their consortia affect their biosynthetic capacities. We performed metagenomic sequencing for 22 Oscillatoriales-dominated (Cyanobacteria) microbial mats collected across the Eel River network in Northern California and investigated factors associated with anatoxin-a producing cyanobacteria. All microbial communities were dominated by one or two cyanobacterial species, so the key mat metabolisms involve oxygenic photosynthesis and carbon oxidation. Only a few metabolisms fueled the growth of the mat communities, with little evidence for anaerobic metabolic pathways. We genomically defined four cyanobacterial species, all which shared <96% average nucleotide identity with reference Oscillatoriales genomes and are potentially novel species in the genus Microcoleus. One of the Microcoleus species contained the anatoxin-a biosynthesis genes, and we describe the first anatoxin-a gene cluster from the Microcoleus clade within Oscillatoriales. Occurrence of these four Microcoleus species in the watershed was correlated with total dissolved nitrogen and phosphorus concentrations, and the species that contains the anatoxin-a gene cluster was found in sites with higher nitrogen concentrations. Microbial assemblages in mat samples with the anatoxin-a gene cluster consistently had a lower abundance of Burkholderiales (Betaproteobacteria) species than did mats without the anatoxin-producing genes. The associations of water nutrient concentrations and certain co-occurring microbes with anatoxin-a producing Microcoleus motivate further exploration for their roles as potential controls on the distributions of toxigenic benthic cyanobacteria in river networks.
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Anatoxin concentrations, algal assemblages, and water quality data for the South Fork Eel, Salmon, and Russian Rivers in northern California, 2022-2023
We collected this data to better understand the timing of peak benthic cyanobacterial mat occurrence (specifically taxa associated with anatoxin production, Microcoleus and Anabaena) and mat anatoxin concentrations in rivers. We sampled in northern California on the South Fork Eel, Salmon, and Russian Rivers biweekly in 2022, and the Salmon River biweekly and South Fork Eel weekly in 2023. During each sampling event, we conducted benthic cover surveys, measured in-situ water quality parameters (temperature, pH, dissolved oxygen, conductivity), and collected surface water samples and targeted cyanobacteria samples. In 2022 on all rivers and in 2023 at the Salmon River, we also collected distributed non-targeted periphyton samples to characterize full-reach community compositions. All sampling was completed in 150-m reaches upstream of sensors recording continuous dissolved oxygen, conductivity, and temperature data. We analyzed surface water samples for nitrate, ammonium, soluble reactive phosphate, total dissolved carbon, and dissolved organic carbon. We also analyzed surface water samples from 2022 for major anions (Cl, SO4, Br) and cations (Na, K, Mg, Ca). Targeted-cyanobacteria and non-target periphyton samples were analyzed for anatoxins, relative abundance of algal taxa (via microscopy), ash-free dry mass, and chlorophyll-a. To estimate mean river depth within the dissolved oxygen footprint upstream of sensors, we kayaked portions of the river and collected river depth measurements. We also measured discharge at each river excluding the Salmon River (due to high discharge) and completed pebble counts at the South Fork Eel River to obtain sediment grain size distributions.
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- PAR ID:
- 10588921
- Publisher / Repository:
- Environmental Data Initiative
- Date Published:
- Format(s):
- Medium: X
- Location:
- South Fork Eel, Salmon, and Russian Rivers in northern California
- Institution:
- University of Nevada, Reno
- Sponsoring Org:
- National Science Foundation
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Abstract Blooms of planktonic cyanobacteria have long been of concern in lakes, but more recently, harmful impacts of riverine benthic cyanobacterial mats been recognized. As yet, we know little about how various benthic cyanobacteria are distributed in river networks, or how environmental conditions or other associated microbes in their consortia affect their biosynthetic capacities. We performed metagenomic sequencing for 22 Oscillatoriales-dominated (Cyanobacteria) microbial mats collected across the Eel River network in Northern California and investigated factors associated with anatoxin-a producing cyanobacteria. All microbial communities were dominated by one or two cyanobacterial species, so the key mat metabolisms involve oxygenic photosynthesis and carbon oxidation. Only a few metabolisms fueled the growth of the mat communities, with little evidence for anaerobic metabolic pathways. We genomically defined four cyanobacterial species, all which shared <96% average nucleotide identity with reference Oscillatoriales genomes and are potentially novel species in the genus Microcoleus. One of the Microcoleus species contained the anatoxin-a biosynthesis genes, and we describe the first anatoxin-a gene cluster from the Microcoleus clade within Oscillatoriales. Occurrence of these four Microcoleus species in the watershed was correlated with total dissolved nitrogen and phosphorus concentrations, and the species that contains the anatoxin-a gene cluster was found in sites with higher nitrogen concentrations. Microbial assemblages in mat samples with the anatoxin-a gene cluster consistently had a lower abundance of Burkholderiales (Betaproteobacteria) species than did mats without the anatoxin-producing genes. The associations of water nutrient concentrations and certain co-occurring microbes with anatoxin-a producing Microcoleus motivate further exploration for their roles as potential controls on the distributions of toxigenic benthic cyanobacteria in river networks.more » « less
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