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Title: Fresnel biprism common-path low-coherence digital holography for dynamic light scattering spectroscopy of biological materials
Doppler frequency shifts associated with the motions in cells range from mHz to Hz, requiring ultra-stable interferometry to capture frequency offsets at several parts in 10^18. Common-path interferometers minimize the influence of mechanical disturbances when the signal and reference share common optical elements. In this paper, multi-mode speckle self-referencing via a Fresnel biprism demonstrates frequency stability down to 1 mHz. A low-coherence NIR source creates an OCT-like pseudo-coherence-gate in Fourier-domain holography without phase stepping, and the Fourier reconstruction of the self-referencing speckle fields produces an image-domain autocorrelation of the target. Fluctuation spectroscopy of dynamic speckle is performed on a semi-solid lipid emulsion that captures Brownian thermal signatures and on feline tissue culture that measures active intracellular transport. The extension of biodynamic imaging to lower frequencies opens the opportunity for studies of cell crawling in macroscopic living tissues.  more » « less
Award ID(s):
2200186
PAR ID:
10624925
Author(s) / Creator(s):
; ; ;
Publisher / Repository:
Optica
Date Published:
Journal Name:
Biomedical Optics Express
Volume:
16
Issue:
2
ISSN:
2156-7085
Page Range / eLocation ID:
806
Format(s):
Medium: X
Sponsoring Org:
National Science Foundation
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