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1. High-speed 3D integral imaging for sensing and visualization of dynamic underwater events

   https://doi.org/10.1364/OPTCON.530264  

   Joshi, Rakesh; Lee, Jiheon; Javidi, Bahram (January 2024, Optics Continuum)

   The study of high-speed phenomena in underwater environments is pivotal across diverse scientific and engineering domains. This paper introduces a high-speed (3D) integral imaging (InIm) based system to 1) visualize high-speed dynamic underwater events, and 2) detect modulated signals for potential optical communication applications. The proposed system is composed of a high-speed camera with a lenslet array-based integral imaging setup to capture and reconstruct 3D images of underwater scenes and detect temporally modulated optical signals. For 3D visualization, we present experiments to capture the elemental images of high-speed underwater events with passive integral imaging, which were then computationally reconstructed to visualize 3D dynamic underwater scenes. We present experiments for 3D imaging and reconstruct the depth map of high-speed underwater dynamic jets of air bubbles, offering depth information and visualizing the 3D movement of these jets. To detect temporally modulated optical signals, we present experiments to demonstrate the ability to capture and reconstruct high-speed underwater modulated optical signals in turbidity. To the best of our knowledge, this is the first report on high-speed underwater 3D integral imaging for 3D visualization and optical signal communication. The findings illustrate the potential of high-speed integral imaging in the visualization and detection of underwater dynamic events, which can be useful in underwater exploration and monitoring. 

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2. Time-Multiplexed Miniaturized Two-Photon Microscopy

   https://doi.org/10.1364/BRAIN.2024.BM3C.2  

   Liu, Shing-Jiuan; Zhang, Zixiao; Mattison, Ben; Yang, Weijian (July 2024, Optica Publishing Group)

   We propose a time-multiplexed miniaturized two-photon microscope (TM-MINI2P), enabling a two-fold increase in imaging speed while maintaining a high spatial resolution. Using TM-MINI2P, we conducted high-speed in-vivo calcium imaging in mouse cortex. 

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3. High-speed two-photon microscopy with adaptive line-excitation

   https://doi.org/10.1101/2024.01.24.577149  

   Li, Y; Guo, S; Mattison, B; Yang, W (January 2024, bioRxiv)

   Abstract We present a two-photon fluorescence microscope designed for high-speed imaging of neural activity in cellular resolution. Our microscope uses a new adaptive sampling scheme with line illumination. Instead of building images pixel by pixel via scanning a diffraction-limited spot across the sample, our scheme only illuminates the regions of interest (i.e., neuronal cell bodies), and samples a large area of them in a single measurement. Such a scheme significantly increases the imaging speed and reduces the overall laser power on the brain tissue. Using this approach, we performed high-speed imaging of the neural activity of mouse cortexin vivo. Our method provides a new sampling strategy in laser-scanning two-photon microscopy, and will be powerful for high-throughput imaging of neural activity. 

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4. High-speed two-photon microscopy with adaptive line-excitation

   https://doi.org/10.1364/OPTICA.529930  

   Li, Yunyang; Guo, Shu; Mattison, Ben; Hu, Junjie; Man, Kwun_Nok Mimi; Yang, Weijian (August 2024, Optica)

   We present a two-photon fluorescence microscope designed for high-speed imaging of neural activity at cellular resolution. Our microscope uses an adaptive sampling scheme with line illumination. Instead of building images pixel by pixel via scanning a diffraction-limited spot across the sample, our scheme only illuminates the regions of interest (i.e., neuronal cell bodies) and samples a large area of them in a single measurement. Such a scheme significantly increases the imaging speed and reduces the overall laser power on the brain tissue. Using this approach, we performed high-speed imaging of the neuronal activity in mouse cortexin vivo. Our method provides a sampling strategy in laser-scanning two-photon microscopy and will be powerful for high-throughput imaging of neural activity. 

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5. High-Speed Neural Imaging with Multiplexed Miniature Two-Photon Microscopy (M-MINI2P)

   https://doi.org/10.1101/2025.03.04.641573  

   Zhang, Zixiao; Liu, Shing-Jiuan; Mattison, Ben; Muir, Jessie; Kim, Christina K; Yang, Weijian (March 2025, bioRxiv)

   SUMMARY Head-mounted miniaturized two-photon microscopes are powerful tools to record neural activity with cellular resolution deep in the mouse brain during unrestrained, free-moving behavior. Two-photon microscopy, however, is traditionally limited in imaging frame rate due to the necessity of raster scanning the laser excitation spot over a large field-of-view (FOV). Here, we present two multiplexed miniature two-photon microscopes (M-MINI2Ps) to increase the imaging frame rate while preserving the spatial resolution. Two different FOVs are imaged simultaneously and then demixed temporally or computationally. We demonstrate large-scale (500×500 µm²FOV) multiplane calcium imaging in visual cortex and prefrontal cortex in freely moving mice for spontaneous activity and auditory stimulus evoked responses. Furthermore, the increased speed of M-MINI2Ps also enables two-photon voltage imaging at 400 Hz over a 380×150 µm²FOV in freely moving mice. M-MINI2Ps have compact footprints and are compatible with the open-source MINI2P. M-MINI2Ps, together with their design principles, allow the capture of faster physiological dynamics and population recordings over a greater volume than currently possible in freely moving mice, and will be a powerful tool in systems neuroscience. 

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