An official website of the United States government
This exercise is intended to provide students with a real-world example of how museum specimens can provide additional context to challenges and considerations in the broader field of applied conservation genomics. After a brief introduction to the study system—the conservation status of Iberian lynx (Lynx pardinus)—students will be asked to review an open-access, peer-reviewed publication that features samples from varied museum, archaeological, and paleontological contexts. Through reading the guide and discussion questions, students will reflect upon study design, concepts, and challenges presented at the intersection between the fields of conservation genomics and museum-based studies. The exercise ends with students breaking down the research into the main components (e.g., research question, independent and dependent variables, hypotheses, predictions) to set a structure for critically reading other scientific studies or designing their own research question.
more »
« less
Applications of Museum Collections and Genomics to Biodiversity Conservation
To address the challenges of sampling endangered or extinct species in the field, many studies have turned to historically underutilized sources of genetic material: natural history museums. Despite the fact that DNA from specimens collected decades or even hundreds of years ago is often fragmented and degraded, research has shown that historical DNA can still be used effectively to infer phylogenetic relationships and intra-specific patterns of population genetic structure. This synthesis aims to provide students and conservation practitioners with a solid understanding of the methodological strategies needed to apply genetic tools to natural history museum specimens. Specifically, we offer clear definitions and essential considerations for designing a conservation genomics project that includes both modern and historical samples. We recommend that instructors use this synthesis to introduce the foundational knowledge required for two companion exercises: “The Application of Conservation Museomics Approaches to the Protection of the Iberian Lynx (Lynx pardinus)” and “Designing a Conservation Genetics Project Incorporating DNA from Museum Specimens.”
more »
« less
- Award ID(s):
- 1926215
- PAR ID:
- 10648722
- Publisher / Repository:
- American Museum of Natural History
- Date Published:
- Journal Name:
- Lessons in Conservation
- Volume:
- 14
- ISSN:
- 1938-7024
- Page Range / eLocation ID:
- 108 to 148
- Format(s):
- Medium: X
- Sponsoring Org:
- National Science Foundation
More Like this
-
-
This exercise is intended to provide students with a real-world example of how museum specimens can provide additional context to challenges and considerations in the broader field of applied conservation genomics. After a brief introduction to the study system—the conservation status of Iberian lynx (Lynx pardinus)—students will be asked to review an open-access, peer-reviewed publication that features samples from varied museum, archaeological, and paleontological contexts. Through reading the guide and discussion questions, students will reflect upon study design, concepts, and challenges presented at the intersection between the fields of conservation genomics and museum-based studies. The exercise ends with students breaking down the research into the main components (e.g., research question, independent and dependent variables, hypotheses, predictions) to set a structure for critically reading other scientific studies or designing their own research question.more » « less
-
null (Ed.)Species that went extinct prior to the genomic era are typically out-of-reach for modern phylogenetic studies. We refer to these as “Alexandrian” extinctions, after the lost library of the ancient world. This is particularly limiting for conservation studies, as genetic data for such taxa may be key to understand extinction threats and risks, the causes of declines, and inform management of related, extant populations. Fortunately, continual advances in biochemistry and DNA sequencing offer increasing ability to recover DNA from historical museum specimens, including fluid-preserved natural history collections. Here, we report on success in recovering nuclear and mitochondrial data from the apparently-extinct subspecies Desmognathus fuscus carri Neill, 1951, a plethodontid salamander from spring runs in central Florida. The two specimens are 50 years old and were likely preserved in unbuffered formalin, but application of a recently derived extraction procedure yielded usable DNA and partially successful Anchored Hybrid Enrichment sequencing. These data suggest that the populations of D. f. carri from peninsular Florida are conspecific with the D. auriculatus A lineage as suggested by previous authors, but likely represented an ecogeographically distinct genetic segment that has now been lost. Genetic data from this Alexandrian extinction thus confirm the geographic extent of population declines and extirpations as well as their ecological context, suggesting a possibly disproportionate loss from sandy-bottom clearwater streams compared to blackwater swamps. Success of these methods bodes well for large-scale application to fluid-preserved natural history specimens from relevant historical populations, but the possibility of significant DNA damage and related sequencing errors in additional hurdle to overcome.more » « less
-
NA (Ed.)We used isotopic and genomic data to explore the ecological and social context of cultural practices associated with the mummification of crocodiles in ancient Egypt. Ancient DNA was recovered from four mummified crocodile hatchlings held in the collections of the Peabody Museum of Natural History, Yale University. Previous genetic analyses of crocodile mummies have indicated that most mummies represent the newly resurrected taxon, Crocodylus suchus Geoffroy Saint-Hilaire, 1807. However, mitogenomic data for the Yale Peabody Museum mum- mies indicates that these specimens represent the first genomically authenticated represen- tatives of the Nile crocodile (Crocodylus niloticus Laurenti, 1768) in museum collections. We explore these findings within the broader context of modern and historical distributions of both crocodile species and the potential implications for our understanding of funerary practices involving crocodiles in ancient Egypt.more » « less
-
Abstract Next‐generation sequencing has greatly expanded the utility and value of museum collections by revealing specimens as genomic resources. As the field of museum genomics grows, so does the need for extraction methods that maximize DNA yields. For avian museum specimens, the established method of extracting DNA from toe pads works well for most specimens. However, for some specimens, especially those of birds that are very small or very large, toe pads can be a poor source of DNA. In this study, we apply two DNA extraction methods (phenol–chloroform and silica column) to three different sources of DNA (toe pad, skin punch and bone) from 10 historical avian museum specimens. We show that a modified phenol–chloroform protocol yielded significantly more DNA than a silica column protocol (e.g., Qiagen DNeasy Blood & Tissue Kit) across all tissue types. However, extractions using the silica column protocol contained longer fragments on average than those using the phenol–chloroform protocol, probably as a result of loss of small fragments through the silica column. While toe pads yielded more DNA than skin punches and bone fragments, skin punches proved to be a reliable alternative source of DNA and might be especially appealing when toe pad extractions are impractical. Overall, we found that historical bird museum specimens contain substantial amounts of DNA for genomic studies under most extraction scenarios, but that a phenol–chloroform protocol consistently provides the high quantities of DNA required for most current genomic protocols.more » « less
- Free Publicly Accessible Full Text
-
Accepted Manuscript1.0
- Journal Article:
- https://doi.org/10.5531/cbc.linc.14.1.7
