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Free, publicly-accessible full text available May 29, 2025
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Insights into chemoautotrophic traits of a prevalent bacterial phylum CSP1-3, herein Sysuimicrobiota
ABSTRACT Candidate bacterial phylum CSP1-3 has not been cultivated and is poorly understood. Here, we analyzed 112 CSP1-3 metagenome-assembled genomes and showed they are likely facultative anaerobes, with 3 of 5 families encoding autotrophy through the reductive glycine pathway (RGP), Wood–Ljungdahl pathway (WLP) or Calvin-Benson-Bassham (CBB), with hydrogen or sulfide as electron donors. Chemoautotrophic enrichments from hot spring sediments and fluorescence in situ hybridization revealed enrichment of six CSP1-3 genera, and both transcribed genes and DNA-stable isotope probing were consistent with proposed chemoautotrophic metabolisms. Ancestral state reconstructions showed that the ancestors of phylum CSP1-3 may have been acetogens that were autotrophic via the RGP, whereas the WLP and CBB were acquired by horizontal gene transfer. Our results reveal that CSP1-3 is a widely distributed phylum with the potential to contribute to the cycling of carbon, sulfur and nitrogen. The name Sysuimicrobiota phy. nov. is proposed.
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Free, publicly-accessible full text available January 2, 2025
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Abstract Background China’s terrestrial ecosystems play a pronounced role in the global carbon cycle. Here we combine spatially-explicit information on vegetation, soil, topography, climate and land use change with a process-based biogeochemistry model to quantify the responses of terrestrial carbon cycle in China during the 20th century.
Results At a century scale, China’s terrestrial ecosystems have acted as a carbon sink averaging at 96 Tg C yr− 1, with large inter-annual and decadal variabilities. The regional sink has been enhanced due to the rising temperature and CO2concentration, with a slight increase trend in carbon sink strength along with the enhanced net primary production in the century. The areas characterized by C source are simulated to extend in the west and north of the Hu Huanyong line, while the eastern and southern regions increase their area and intensity of C sink, particularly in the late 20th century. Forest ecosystems dominate the C sink in China and are responsible for about 64% of the total sink. On the century scale, the increase in carbon sinks in China’s terrestrial ecosystems is mainly contributed by rising CO2. Afforestation and reforestation promote an increase in terrestrial carbon uptake in China from 1950s. Although climate change has generally contributed to the increase of carbon sinks in terrestrial ecosystems in China, the positive effect of climate change has been diminishing in the last decades of the 20th century.
Conclusion This study focuses on the impacts of climate, CO2and land use change on the carbon cycle, and presents the potential trends of terrestrial ecosystem carbon balance in China at a century scale. While a slight increase in carbon sink strength benefits from the enhanced vegetation carbon uptake in China’s terrestrial ecosystems during the 20th century, the increase trend may diminish or even change to a decrease trend under future climate change.
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The wireless signal propagates via multipath arising from different reflections and penetration between a transmitter and receiver. Extracting multipath profiles (e.g., delay and Doppler along each path) from received signals enables many important applications, such as channel prediction and crossband channel estimation (i.e., estimating the channel on a different frequency). The benefit of multipath estimation further increases with mobility since the channel in that case is less stable and more important to track. Yet high-speed mobility poses significant challenges to multipath estimation. In this paper, instead of using time-frequency domain channel representation, we leverage the delay-Doppler domain representation to accurately extract and predict multipath properties. Specifically, we use impulses in the delay-Doppler domain as pilots to estimate the multipath parameters and apply the multipath information to predicting wireless channels as an example application. Our design rationale is that mobility is more predictable than the wireless channel since mobility has inertial while the wireless channel is the outcome of a complicated interaction between mobility, multipath, and noise. We evaluate our approach via both acoustic and RF experiments, including vehicular experiments using USRP. Our results show that the estimated multipath matches the ground truth, and the resulting channel prediction is more accurate than the traditional channel prediction schemes.more » « less